Inhibitory Mechanism of Epsilon-poly-L-Lysine against Botrytis cinerea in Apples
The aim of the study was to investigate the inhibitory effect and mechanism of ε-poly-L-lysine on Botrytis cinerea causing gray mold in apples. The effects of different concentrations of ε-PL on colony diameter, spore germination, mycelial growth of B. cinerea, and its effect on controlling gray mol...
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The editorial department of Science and Technology of Food Industry
2025-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080121 |
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| author | Yong DOU Jing DONG Cheng CHEN Peihong HU Xinchi LIU Lingwei KONG |
| author_facet | Yong DOU Jing DONG Cheng CHEN Peihong HU Xinchi LIU Lingwei KONG |
| author_sort | Yong DOU |
| collection | DOAJ |
| description | The aim of the study was to investigate the inhibitory effect and mechanism of ε-poly-L-lysine on Botrytis cinerea causing gray mold in apples. The effects of different concentrations of ε-PL on colony diameter, spore germination, mycelial growth of B. cinerea, and its effect on controlling gray mold in apples were studied. The changes in cell permeability, antioxidant enzyme activities, cellular soluble protein and nucleic acid leakage, malonaldehyde content, and reactive oxygen species of B. cinerea were also investigated. The results showed that the minimum inhibitory concentration of ε-PL against B. cinerea was 400 mg/L, and the concentration of ε-PL could effectively control gray mold in apples. ε-PL concentration above 100 mg/L could significantly inhibit the spore germination and mycelium growth of B. cinerea (P<0.05), damage the spore membrane structure, and cause the leakage of soluble protein and nucleic acid, resulting in atrophic, small, and even dissolving spores. In addition, ε-PL concentration above 100 mg/L could significantly reduce the activities of superoxide dismutase, polyphenol oxidase, and catalase of B. cinerea and increase the content of malonaldehyde (P<0.05). In conclusion, ε-PL could damage the cell membrane structure of B. cinerea spores, increase cell permeability, and inhibit the activities of antioxidant enzymes, promote the accumulation of reactive oxygen species. This increased the degree of lipid peroxidation of the spore membrane, damaged the spore structure, and ultimately inhibited the growth of B. cinerea. This study laid a foundation for applying ε-PL in the prevention and control of postharvest diseases of apples and had high application value. |
| format | Article |
| id | doaj-art-080a901658254b469efdde6305aa833e |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-080a901658254b469efdde6305aa833e2025-01-21T07:24:08ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-02-0146319620310.13386/j.issn1002-0306.20240801212024080121-3Inhibitory Mechanism of Epsilon-poly-L-Lysine against Botrytis cinerea in ApplesYong DOU0Jing DONG1Cheng CHEN2Peihong HU3Xinchi LIU4Lingwei KONG5Department of Grain Engineering and Food & Drug, Jiangsu Vocational College of Finance & Economics, Huaian 223003, ChinaDepartment of Grain Engineering and Food & Drug, Jiangsu Vocational College of Finance & Economics, Huaian 223003, ChinaCollege of Health Sciences, Jiangsu Vocational Institute of Commerce, Nanjing 211168, ChinaDepartment of Technology, Huaian Zhengchang Feed Co., Ltd., Huaian 223005, ChinaDepartment of Grain Engineering and Food & Drug, Jiangsu Vocational College of Finance & Economics, Huaian 223003, ChinaHuaian Kuailu Milk Co., Ltd., Huaian 223001, ChinaThe aim of the study was to investigate the inhibitory effect and mechanism of ε-poly-L-lysine on Botrytis cinerea causing gray mold in apples. The effects of different concentrations of ε-PL on colony diameter, spore germination, mycelial growth of B. cinerea, and its effect on controlling gray mold in apples were studied. The changes in cell permeability, antioxidant enzyme activities, cellular soluble protein and nucleic acid leakage, malonaldehyde content, and reactive oxygen species of B. cinerea were also investigated. The results showed that the minimum inhibitory concentration of ε-PL against B. cinerea was 400 mg/L, and the concentration of ε-PL could effectively control gray mold in apples. ε-PL concentration above 100 mg/L could significantly inhibit the spore germination and mycelium growth of B. cinerea (P<0.05), damage the spore membrane structure, and cause the leakage of soluble protein and nucleic acid, resulting in atrophic, small, and even dissolving spores. In addition, ε-PL concentration above 100 mg/L could significantly reduce the activities of superoxide dismutase, polyphenol oxidase, and catalase of B. cinerea and increase the content of malonaldehyde (P<0.05). In conclusion, ε-PL could damage the cell membrane structure of B. cinerea spores, increase cell permeability, and inhibit the activities of antioxidant enzymes, promote the accumulation of reactive oxygen species. This increased the degree of lipid peroxidation of the spore membrane, damaged the spore structure, and ultimately inhibited the growth of B. cinerea. This study laid a foundation for applying ε-PL in the prevention and control of postharvest diseases of apples and had high application value.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080121ε-poly-l-lysinebacteriostatic mechanismgray mold in applescell leakageantioxidant oxidase |
| spellingShingle | Yong DOU Jing DONG Cheng CHEN Peihong HU Xinchi LIU Lingwei KONG Inhibitory Mechanism of Epsilon-poly-L-Lysine against Botrytis cinerea in Apples Shipin gongye ke-ji ε-poly-l-lysine bacteriostatic mechanism gray mold in apples cell leakage antioxidant oxidase |
| title | Inhibitory Mechanism of Epsilon-poly-L-Lysine against Botrytis cinerea in Apples |
| title_full | Inhibitory Mechanism of Epsilon-poly-L-Lysine against Botrytis cinerea in Apples |
| title_fullStr | Inhibitory Mechanism of Epsilon-poly-L-Lysine against Botrytis cinerea in Apples |
| title_full_unstemmed | Inhibitory Mechanism of Epsilon-poly-L-Lysine against Botrytis cinerea in Apples |
| title_short | Inhibitory Mechanism of Epsilon-poly-L-Lysine against Botrytis cinerea in Apples |
| title_sort | inhibitory mechanism of epsilon poly l lysine against botrytis cinerea in apples |
| topic | ε-poly-l-lysine bacteriostatic mechanism gray mold in apples cell leakage antioxidant oxidase |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080121 |
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