Transcriptome and Physiological Analyses of Resistant and Susceptible Pepper (<i>Capsicum annuum</i>) to <i>Verticillium dahliae</i> Inoculum

Transcriptome and Physiological Analyses of Resistant and Susceptible Pepper (<i>Capsicum annuum</i>) to <i>Verticillium dahliae</i> Inoculum

Pepper (<i>Capsicum annuum</i>) is a globally important vegetable, and Verticillium wilt is an important disease affecting peppers and is caused by <i>Verticillium dahliae</i>, which can severely reduce yields. However, the molecular mechanisms underlying the responses of pep...

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Bibliographic Details
Main Authors: Xinmin Huang, Liming He, Huimin Tan, Jiayi Liu, Qiucheng Qiu, Qidi Sun, Lejun Ouyang, Hanbing Han, Qinqin He
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Horticulturae
Subjects:
pepper
Verticillium wilt
<i>Verticillium dahliae</i>
chlorophyll content
malondialdehyde concentration
differentially expressed genes
Online Access:https://www.mdpi.com/2311-7524/10/11/1160
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Summary:Pepper (<i>Capsicum annuum</i>) is a globally important vegetable, and Verticillium wilt is an important disease affecting peppers and is caused by <i>Verticillium dahliae</i>, which can severely reduce yields. However, the molecular mechanisms underlying the responses of pepper to infection by <i>V. dahliae</i> are largely unknown. We performed physiological and transcriptome analysis using resistant and susceptible pepper cultivars inoculated with <i>V. dahliae</i>. Compared to the susceptible cultivar MS66, the resistant cultivar MS72 retained higher chlorophyll content and lower malondialdehyde content after inoculation. At 3 days after inoculation (DAI), compared with MS66, 534 differentially expressed genes (DEGs) were identified in MS72. At 5 DAI, 2392 DEGs were identified in MS72 compared with MS66. The DEGs in MS72 were mainly enriched in the cell wall and photosynthesis-related Gene Ontology terms, as well as in pathways such as cutin, suberin, wax biosynthesis, phenylpropanoid biosynthesis, and photosynthesis. Using weighted gene co-expression network analysis, 36 hub genes involved in the resistance response were identified, including the transcription factor <i>bHLH93</i> (Capana04g000815), defense-like protein 1 (MSTRG.5904), and miraculin-like (Capana10g002167). Our findings contribute to a more comprehensive understanding of the response mechanism of pepper to <i>V. dahliae</i> inoculation, providing new avenues for improving pepper resistance through breeding programs.
ISSN:2311-7524

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