Defects at the Posttranscriptional Level Account for the Low TCRζ Chain Expression Detected in Gastric Cancer Independently of Caspase-3 Activity
Background. Reduced TCRζ chain surface has been reported in T cells from patients with different inflammatory conditions and cancer. However, the causes of this diminished expression in cancer remain elusive. Methods. T cell-enriched populations of blood or tissue (tumoral and nontumoral) origin fro...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/1039458 |
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| author | Ana Aguinaga-Barrilero Patricia Castro-Sánchez Ignacio Juárez Alberto Gutiérrez-Calvo Noelia Rodríguez-Pérez Adela Lopez Remedios Gómez José M. Martin-Villa |
| author_facet | Ana Aguinaga-Barrilero Patricia Castro-Sánchez Ignacio Juárez Alberto Gutiérrez-Calvo Noelia Rodríguez-Pérez Adela Lopez Remedios Gómez José M. Martin-Villa |
| author_sort | Ana Aguinaga-Barrilero |
| collection | DOAJ |
| description | Background. Reduced TCRζ chain surface has been reported in T cells from patients with different inflammatory conditions and cancer. However, the causes of this diminished expression in cancer remain elusive. Methods. T cell-enriched populations of blood or tissue (tumoral and nontumoral) origin from 44 patients with gastric adenocarcinoma and 33 healthy subjects were obtained. Samples were subjected to cytofluorimetry, Western blot analysis, TCRζ cDNA sequencing experiments, measurement of TCRζ mRNA levels, and caspase-3 activity assays. Results. Cytofluorimetry revealed a decreased TCRζ expression in T cells of patients, assessed either as percentage of cells expressing this chain (blood: control subjects 99.8±0.1%, patients 98.8±1.1%P<0.001; tissue: control subjects 96.7±0.9%, patients tumoral tissue 67.9±27.0%, patients nontumoral tissue 82.8±12.6%, P=0.019) or mean fluorescence intensity (MFI) value (blood: control subjects 102.2±26.0; patients 58.0±12.3, P=0.001; tissue: control subjects 99.4±21.4; patients tumoral tissue 41.6±21.4; patients nontumoral tissue 62.3±16.6, P=0.001). Other chains pertaining to the TCR-CD3 complex (CD3ε) showed no significant differences (MFI values). Subsequent TCRζ cDNA sequencing experiments or measurements of TCRζ mRNA levels disclosed no differences between patients and control subjects. Evaluation of caspase-3 activity showed higher levels in T cell extracts of patients, and this activity could be decreased by 70% with the use of the inhibitor Ac-DEVD-FMK, although CD3ζ expression levels did not recover. Conclusions. These results further place the defect responsible for the low TCRζ expression in cancer at the posttranscriptional level and suggests contrary to what has been proposed in other pathologies that elevated caspase-3 activity is not the causative agent. |
| format | Article |
| id | doaj-art-07fb59270df346d0ba693f5068494631 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-07fb59270df346d0ba693f50684946312025-02-03T06:43:35ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/10394581039458Defects at the Posttranscriptional Level Account for the Low TCRζ Chain Expression Detected in Gastric Cancer Independently of Caspase-3 ActivityAna Aguinaga-Barrilero0Patricia Castro-Sánchez1Ignacio Juárez2Alberto Gutiérrez-Calvo3Noelia Rodríguez-Pérez4Adela Lopez5Remedios Gómez6José M. Martin-Villa7Inmunología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, SpainInmunología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, SpainInmunología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, SpainServicio de Cirugía General y Aparato Digestivo, Hospital Universitario Príncipe de Asturias, Alcalá de Henares, Madrid, SpainInmunología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, SpainServicio de Cirugía General y Aparato Digestivo, Hospital Universitario Príncipe de Asturias, Alcalá de Henares, Madrid, SpainServicio de Cirugía General y Aparato Digestivo, Hospital Universitario Príncipe de Asturias, Alcalá de Henares, Madrid, SpainInmunología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, SpainBackground. Reduced TCRζ chain surface has been reported in T cells from patients with different inflammatory conditions and cancer. However, the causes of this diminished expression in cancer remain elusive. Methods. T cell-enriched populations of blood or tissue (tumoral and nontumoral) origin from 44 patients with gastric adenocarcinoma and 33 healthy subjects were obtained. Samples were subjected to cytofluorimetry, Western blot analysis, TCRζ cDNA sequencing experiments, measurement of TCRζ mRNA levels, and caspase-3 activity assays. Results. Cytofluorimetry revealed a decreased TCRζ expression in T cells of patients, assessed either as percentage of cells expressing this chain (blood: control subjects 99.8±0.1%, patients 98.8±1.1%P<0.001; tissue: control subjects 96.7±0.9%, patients tumoral tissue 67.9±27.0%, patients nontumoral tissue 82.8±12.6%, P=0.019) or mean fluorescence intensity (MFI) value (blood: control subjects 102.2±26.0; patients 58.0±12.3, P=0.001; tissue: control subjects 99.4±21.4; patients tumoral tissue 41.6±21.4; patients nontumoral tissue 62.3±16.6, P=0.001). Other chains pertaining to the TCR-CD3 complex (CD3ε) showed no significant differences (MFI values). Subsequent TCRζ cDNA sequencing experiments or measurements of TCRζ mRNA levels disclosed no differences between patients and control subjects. Evaluation of caspase-3 activity showed higher levels in T cell extracts of patients, and this activity could be decreased by 70% with the use of the inhibitor Ac-DEVD-FMK, although CD3ζ expression levels did not recover. Conclusions. These results further place the defect responsible for the low TCRζ expression in cancer at the posttranscriptional level and suggests contrary to what has been proposed in other pathologies that elevated caspase-3 activity is not the causative agent.http://dx.doi.org/10.1155/2020/1039458 |
| spellingShingle | Ana Aguinaga-Barrilero Patricia Castro-Sánchez Ignacio Juárez Alberto Gutiérrez-Calvo Noelia Rodríguez-Pérez Adela Lopez Remedios Gómez José M. Martin-Villa Defects at the Posttranscriptional Level Account for the Low TCRζ Chain Expression Detected in Gastric Cancer Independently of Caspase-3 Activity Journal of Immunology Research |
| title | Defects at the Posttranscriptional Level Account for the Low TCRζ Chain Expression Detected in Gastric Cancer Independently of Caspase-3 Activity |
| title_full | Defects at the Posttranscriptional Level Account for the Low TCRζ Chain Expression Detected in Gastric Cancer Independently of Caspase-3 Activity |
| title_fullStr | Defects at the Posttranscriptional Level Account for the Low TCRζ Chain Expression Detected in Gastric Cancer Independently of Caspase-3 Activity |
| title_full_unstemmed | Defects at the Posttranscriptional Level Account for the Low TCRζ Chain Expression Detected in Gastric Cancer Independently of Caspase-3 Activity |
| title_short | Defects at the Posttranscriptional Level Account for the Low TCRζ Chain Expression Detected in Gastric Cancer Independently of Caspase-3 Activity |
| title_sort | defects at the posttranscriptional level account for the low tcrζ chain expression detected in gastric cancer independently of caspase 3 activity |
| url | http://dx.doi.org/10.1155/2020/1039458 |
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