An Inactivated West Nile Virus Vaccine Candidate Based on the Lineage 2 Strain
<b>Background</b>: West Nile virus (WNV) is a rapidly growing problem worldwide. The lack of emergency treatment and a safe licensed vaccine against WNV allows the virus to cause sporadic outbreaks of human disease, including fatal cases. Formalin-inactivated vaccines have been used for...
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MDPI AG
2024-12-01
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| author | Mikhail F. Vorovitch Ksenia K. Tuchynskaya Yuriy A. Kruglov Nikita S. Peunkov Guzal F. Mostipanova Ivan S. Kholodilov Alla L. Ivanova Maria P. Fedina Larissa V. Gmyl Evgeny S. Morozkin German V. Roev Lyudmila S. Karan Galina G. Karganova |
| author_facet | Mikhail F. Vorovitch Ksenia K. Tuchynskaya Yuriy A. Kruglov Nikita S. Peunkov Guzal F. Mostipanova Ivan S. Kholodilov Alla L. Ivanova Maria P. Fedina Larissa V. Gmyl Evgeny S. Morozkin German V. Roev Lyudmila S. Karan Galina G. Karganova |
| author_sort | Mikhail F. Vorovitch |
| collection | DOAJ |
| description | <b>Background</b>: West Nile virus (WNV) is a rapidly growing problem worldwide. The lack of emergency treatment and a safe licensed vaccine against WNV allows the virus to cause sporadic outbreaks of human disease, including fatal cases. Formalin-inactivated vaccines have been used for a long time and have been shown to be very safe and effective, especially in susceptible populations. <b>Methods:</b> By adapting tick-borne encephalitis vaccine production technology, we produced a laboratory-inactivated vaccine against WNV based on the strain SHUA, isolated from humans with a lethal WNV infection in the year 2021. <b>Results</b>: The potential vaccine was tested for safety in vitro and in vivo in outbred SHK mice of different ages, including PCR analysis of the brains of these mice to test for the absence of viral RNA after intracerebral injection. <b>Conclusions:</b> The inactivated whole-virion laboratory vaccine showed 100% seroconversion and immunogenicity against WNV strain SHUA-1, isolated from a lethal human case, and provided the mice with 100% protection from disease and death. |
| format | Article |
| id | doaj-art-07c50f1a9aad40cd94c74df95db221fc |
| institution | OA Journals |
| issn | 2076-393X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj-art-07c50f1a9aad40cd94c74df95db221fc2025-08-20T02:01:29ZengMDPI AGVaccines2076-393X2024-12-011212139810.3390/vaccines12121398An Inactivated West Nile Virus Vaccine Candidate Based on the Lineage 2 StrainMikhail F. Vorovitch0Ksenia K. Tuchynskaya1Yuriy A. Kruglov2Nikita S. Peunkov3Guzal F. Mostipanova4Ivan S. Kholodilov5Alla L. Ivanova6Maria P. Fedina7Larissa V. Gmyl8Evgeny S. Morozkin9German V. Roev10Lyudmila S. Karan11Galina G. Karganova12Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, RussiaChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, RussiaChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, RussiaChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, RussiaChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, RussiaChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, RussiaChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, RussiaChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, RussiaChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, RussiaFederal Budget Institute of Science «Central Research Institute of Epidemiology» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow 111123, RussiaFederal Budget Institute of Science «Central Research Institute of Epidemiology» of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow 111123, RussiaResearch Center of Neurology, Moscow 125367, RussiaChumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, Russia<b>Background</b>: West Nile virus (WNV) is a rapidly growing problem worldwide. The lack of emergency treatment and a safe licensed vaccine against WNV allows the virus to cause sporadic outbreaks of human disease, including fatal cases. Formalin-inactivated vaccines have been used for a long time and have been shown to be very safe and effective, especially in susceptible populations. <b>Methods:</b> By adapting tick-borne encephalitis vaccine production technology, we produced a laboratory-inactivated vaccine against WNV based on the strain SHUA, isolated from humans with a lethal WNV infection in the year 2021. <b>Results</b>: The potential vaccine was tested for safety in vitro and in vivo in outbred SHK mice of different ages, including PCR analysis of the brains of these mice to test for the absence of viral RNA after intracerebral injection. <b>Conclusions:</b> The inactivated whole-virion laboratory vaccine showed 100% seroconversion and immunogenicity against WNV strain SHUA-1, isolated from a lethal human case, and provided the mice with 100% protection from disease and death.https://www.mdpi.com/2076-393X/12/12/1398West Nile virusWest Nile feverinactivated vaccineimmunogenicityseroconversionneutralizing antibodies |
| spellingShingle | Mikhail F. Vorovitch Ksenia K. Tuchynskaya Yuriy A. Kruglov Nikita S. Peunkov Guzal F. Mostipanova Ivan S. Kholodilov Alla L. Ivanova Maria P. Fedina Larissa V. Gmyl Evgeny S. Morozkin German V. Roev Lyudmila S. Karan Galina G. Karganova An Inactivated West Nile Virus Vaccine Candidate Based on the Lineage 2 Strain Vaccines West Nile virus West Nile fever inactivated vaccine immunogenicity seroconversion neutralizing antibodies |
| title | An Inactivated West Nile Virus Vaccine Candidate Based on the Lineage 2 Strain |
| title_full | An Inactivated West Nile Virus Vaccine Candidate Based on the Lineage 2 Strain |
| title_fullStr | An Inactivated West Nile Virus Vaccine Candidate Based on the Lineage 2 Strain |
| title_full_unstemmed | An Inactivated West Nile Virus Vaccine Candidate Based on the Lineage 2 Strain |
| title_short | An Inactivated West Nile Virus Vaccine Candidate Based on the Lineage 2 Strain |
| title_sort | inactivated west nile virus vaccine candidate based on the lineage 2 strain |
| topic | West Nile virus West Nile fever inactivated vaccine immunogenicity seroconversion neutralizing antibodies |
| url | https://www.mdpi.com/2076-393X/12/12/1398 |
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