Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients
Objectives. To investigate the association of serum autoantibodies against M2-muscarinic acetylcholine receptor (anti-M2-R) with atrial fibrosis in long-standing persistent atrial fibrillation (AF) patients. Methods. Twenty-four long-standing persistent AF patients, scheduled to undergo hybrid ablat...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Cardiology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2019/8271871 |
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| author | Guiling Ma Xuejiao Wu Lijun Zeng Jiawei Jin Xingpeng Liu Jianjun Zhang Lin Zhang |
| author_facet | Guiling Ma Xuejiao Wu Lijun Zeng Jiawei Jin Xingpeng Liu Jianjun Zhang Lin Zhang |
| author_sort | Guiling Ma |
| collection | DOAJ |
| description | Objectives. To investigate the association of serum autoantibodies against M2-muscarinic acetylcholine receptor (anti-M2-R) with atrial fibrosis in long-standing persistent atrial fibrillation (AF) patients. Methods. Twenty-four long-standing persistent AF patients, scheduled to undergo hybrid ablation surgery, were enrolled in the study. Twenty-six patients with sinus rhythm, scheduled to undergo coronary artery bypass grafting surgery, were enrolled into the non-AF group. We detected serum anti-M2-R levels. Left atrial appendages were subjected to histological and molecular biological assays. Patients in the AF group received follow-up for two years. Results. The AF group showed significantly higher serum anti-M2-R levels compared to the non-AF group (496.2 ± 232.5 vs. 86.3 ± 25.7 pmol/L, p<0.001). The AF group exhibited severe fibrosis in the left atrial appendages, as indicated by increased collagen volume fraction (45.2 ± 4.7% vs. 27.6 ± 8.3%, p<0.001), and higher levels of collagen I (0.52 ± 0.04 vs. 0.24 ± 0.06, p<0.001) and collagen III (0.51 ± 0.07 vs. 0.36 ± 0.09, p<0.001). TGF-β1 and CTGF were also upregulated in the AF group. A positive correlation between serum anti-M2-R levels and fibrosis of the left atrial appendage and fibrogenic indexes was observed. Conclusions. Serum anti-M2-R levels are higher in AF patients and are associated with the severity of atrial fibrosis. In addition, serum anti-M2-R levels are positively correlated to TGF-β1 and CTGF expression in the left atrial appendage. |
| format | Article |
| id | doaj-art-07b9951bd9f543758f91ebaf86726ded |
| institution | Kabale University |
| issn | 2090-8016 2090-0597 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiology Research and Practice |
| spelling | doaj-art-07b9951bd9f543758f91ebaf86726ded2025-02-03T05:50:22ZengWileyCardiology Research and Practice2090-80162090-05972019-01-01201910.1155/2019/82718718271871Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation PatientsGuiling Ma0Xuejiao Wu1Lijun Zeng2Jiawei Jin3Xingpeng Liu4Jianjun Zhang5Lin Zhang6Heart Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, ChinaHeart Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, ChinaHeart Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, ChinaInstitute for Medical Research, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, ChinaHeart Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, ChinaHeart Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, ChinaHeart Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, ChinaObjectives. To investigate the association of serum autoantibodies against M2-muscarinic acetylcholine receptor (anti-M2-R) with atrial fibrosis in long-standing persistent atrial fibrillation (AF) patients. Methods. Twenty-four long-standing persistent AF patients, scheduled to undergo hybrid ablation surgery, were enrolled in the study. Twenty-six patients with sinus rhythm, scheduled to undergo coronary artery bypass grafting surgery, were enrolled into the non-AF group. We detected serum anti-M2-R levels. Left atrial appendages were subjected to histological and molecular biological assays. Patients in the AF group received follow-up for two years. Results. The AF group showed significantly higher serum anti-M2-R levels compared to the non-AF group (496.2 ± 232.5 vs. 86.3 ± 25.7 pmol/L, p<0.001). The AF group exhibited severe fibrosis in the left atrial appendages, as indicated by increased collagen volume fraction (45.2 ± 4.7% vs. 27.6 ± 8.3%, p<0.001), and higher levels of collagen I (0.52 ± 0.04 vs. 0.24 ± 0.06, p<0.001) and collagen III (0.51 ± 0.07 vs. 0.36 ± 0.09, p<0.001). TGF-β1 and CTGF were also upregulated in the AF group. A positive correlation between serum anti-M2-R levels and fibrosis of the left atrial appendage and fibrogenic indexes was observed. Conclusions. Serum anti-M2-R levels are higher in AF patients and are associated with the severity of atrial fibrosis. In addition, serum anti-M2-R levels are positively correlated to TGF-β1 and CTGF expression in the left atrial appendage.http://dx.doi.org/10.1155/2019/8271871 |
| spellingShingle | Guiling Ma Xuejiao Wu Lijun Zeng Jiawei Jin Xingpeng Liu Jianjun Zhang Lin Zhang Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients Cardiology Research and Practice |
| title | Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients |
| title_full | Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients |
| title_fullStr | Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients |
| title_full_unstemmed | Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients |
| title_short | Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients |
| title_sort | association of autoantibodies against m2 muscarinic acetylcholine receptor with atrial fibrosis in atrial fibrillation patients |
| url | http://dx.doi.org/10.1155/2019/8271871 |
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