Toxicological Assessment of Pseudospondias microcarpa (A. Rich.) Engl. Hydroethanolic Leaf Extract in Rats: Haematological, Biochemical, and Histopathological Studies
Pseudospondias microcarpa is used traditionally for treating various diseases. However, although parts of the plant are extensively used in African traditional medicine, no scientific study has been reported on its toxicity. Therefore, this study evaluated the acute and subacute toxicity studies of...
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Wiley
2018-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2018/4256782 |
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| author | Donatus Wewura Adongo Priscilla Kolibea Mante Kennedy Kwami Edem Kukuia Charles Kwaku Benneh Robert Peter Biney Eric Boakye-Gyasi Nicholas Akinwale Titiloye Eric Woode |
| author_facet | Donatus Wewura Adongo Priscilla Kolibea Mante Kennedy Kwami Edem Kukuia Charles Kwaku Benneh Robert Peter Biney Eric Boakye-Gyasi Nicholas Akinwale Titiloye Eric Woode |
| author_sort | Donatus Wewura Adongo |
| collection | DOAJ |
| description | Pseudospondias microcarpa is used traditionally for treating various diseases. However, although parts of the plant are extensively used in African traditional medicine, no scientific study has been reported on its toxicity. Therefore, this study evaluated the acute and subacute toxicity studies of the ethanolic extract of P. microcarpa in rats. Male Sprague-Dawley rats (120–150 g) were treated orally with the extract (30, 100, 300, 1000, and 3000 mg kg−1) or distilled water (10 ml kg−1) for 2 weeks and observed daily for general appearance and signs of toxicity. In addition, blood was collected for both biochemical and haematological assays. Sections of tissues from liver, kidney, spleen, brain, and stomach were also used for histopathological examination. Administration of the extract for 14 consecutive days caused no deaths, with an LD50 above 3000 mg kg−1. Except for lymphocytes (%) that showed a significant decrease (F5,23=3.93, P=0.013), all other haematological parameters remained unaffected by the extract. The extract at 100 mg kg−1 showed a significant decrease in the levels of triglyceride and very-low-density lipoproteins (both at P<0.05). Weight change as well as histological evaluation of the organs indicated no toxicity. The study demonstrates that an ethanolic extract of P. microcarpa given orally to rats is safe. |
| format | Article |
| id | doaj-art-07a4f30602174ebf8c1506d0946621a3 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-07a4f30602174ebf8c1506d0946621a32025-02-03T06:05:58ZengWileyThe Scientific World Journal2356-61401537-744X2018-01-01201810.1155/2018/42567824256782Toxicological Assessment of Pseudospondias microcarpa (A. Rich.) Engl. Hydroethanolic Leaf Extract in Rats: Haematological, Biochemical, and Histopathological StudiesDonatus Wewura Adongo0Priscilla Kolibea Mante1Kennedy Kwami Edem Kukuia2Charles Kwaku Benneh3Robert Peter Biney4Eric Boakye-Gyasi5Nicholas Akinwale Titiloye6Eric Woode7Department of Pharmacology, School of Medicine, University of Health and Allied Sciences, Ho, GhanaDepartment of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Pharmacology and Toxicology, University of Ghana School of Pharmacy, College of Health Sciences, University of Ghana, Accra, GhanaDepartment of Pharmacology and Toxicology, School of Pharmacy, University of Health and Allied Sciences, Ho, GhanaDepartment of Pharmacology, School of Medical Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Pathology, School of Medical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaDepartment of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, GhanaPseudospondias microcarpa is used traditionally for treating various diseases. However, although parts of the plant are extensively used in African traditional medicine, no scientific study has been reported on its toxicity. Therefore, this study evaluated the acute and subacute toxicity studies of the ethanolic extract of P. microcarpa in rats. Male Sprague-Dawley rats (120–150 g) were treated orally with the extract (30, 100, 300, 1000, and 3000 mg kg−1) or distilled water (10 ml kg−1) for 2 weeks and observed daily for general appearance and signs of toxicity. In addition, blood was collected for both biochemical and haematological assays. Sections of tissues from liver, kidney, spleen, brain, and stomach were also used for histopathological examination. Administration of the extract for 14 consecutive days caused no deaths, with an LD50 above 3000 mg kg−1. Except for lymphocytes (%) that showed a significant decrease (F5,23=3.93, P=0.013), all other haematological parameters remained unaffected by the extract. The extract at 100 mg kg−1 showed a significant decrease in the levels of triglyceride and very-low-density lipoproteins (both at P<0.05). Weight change as well as histological evaluation of the organs indicated no toxicity. The study demonstrates that an ethanolic extract of P. microcarpa given orally to rats is safe.http://dx.doi.org/10.1155/2018/4256782 |
| spellingShingle | Donatus Wewura Adongo Priscilla Kolibea Mante Kennedy Kwami Edem Kukuia Charles Kwaku Benneh Robert Peter Biney Eric Boakye-Gyasi Nicholas Akinwale Titiloye Eric Woode Toxicological Assessment of Pseudospondias microcarpa (A. Rich.) Engl. Hydroethanolic Leaf Extract in Rats: Haematological, Biochemical, and Histopathological Studies The Scientific World Journal |
| title | Toxicological Assessment of Pseudospondias microcarpa (A. Rich.) Engl. Hydroethanolic Leaf Extract in Rats: Haematological, Biochemical, and Histopathological Studies |
| title_full | Toxicological Assessment of Pseudospondias microcarpa (A. Rich.) Engl. Hydroethanolic Leaf Extract in Rats: Haematological, Biochemical, and Histopathological Studies |
| title_fullStr | Toxicological Assessment of Pseudospondias microcarpa (A. Rich.) Engl. Hydroethanolic Leaf Extract in Rats: Haematological, Biochemical, and Histopathological Studies |
| title_full_unstemmed | Toxicological Assessment of Pseudospondias microcarpa (A. Rich.) Engl. Hydroethanolic Leaf Extract in Rats: Haematological, Biochemical, and Histopathological Studies |
| title_short | Toxicological Assessment of Pseudospondias microcarpa (A. Rich.) Engl. Hydroethanolic Leaf Extract in Rats: Haematological, Biochemical, and Histopathological Studies |
| title_sort | toxicological assessment of pseudospondias microcarpa a rich engl hydroethanolic leaf extract in rats haematological biochemical and histopathological studies |
| url | http://dx.doi.org/10.1155/2018/4256782 |
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