Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD)
Urine-derived mesenchymal stromal/stem cells (USCs) could be a valuable source of cells in regenerative medicine because urine can be easily collected non-invasively. In this paper, USCs were isolated from both healthy dogs and dogs affected by chronic kidney disease (CKD), and the efficacy of colle...
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2025-01-01
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| author | Anna Lange-Consiglio Filippo Tagliasacchi Fausto Cremonesi Claudia Gusmara Claudia Pollera Paola Scarpa Giulia Gaspari Pietro Riccaboni |
| author_facet | Anna Lange-Consiglio Filippo Tagliasacchi Fausto Cremonesi Claudia Gusmara Claudia Pollera Paola Scarpa Giulia Gaspari Pietro Riccaboni |
| author_sort | Anna Lange-Consiglio |
| collection | DOAJ |
| description | Urine-derived mesenchymal stromal/stem cells (USCs) could be a valuable source of cells in regenerative medicine because urine can be easily collected non-invasively. In this paper, USCs were isolated from both healthy dogs and dogs affected by chronic kidney disease (CKD), and the efficacy of collection methods (spontaneous micturition, bladder catheterization, and cystocentesis) were compared. Isolated cells were cultured in the presence of platelet-rich plasma and studied for their proliferative capacity (growth curve, doubling time, and colony forming unit), differentiation properties, expression of mesenchymal markers, and Klotho protein. Morphologically, all cells were elongated and fibroblast-like. USCs isolated from samples collected by spontaneous micturition and bladder catheterization failed to proliferate, whilst USCs obtained by cystocentesis showed a doubling time of 2.04 days in healthy dogs and 1.7 days in dogs with CKD (<i>p</i> < 0.05). Cells were able to differentiate into osteogenic, chondrogenic, and adipogenic lines, showed positive expression to mesenchymal/stem markers, negative expression to hematopoietic markers, and major histocompatibility complex (MHCII) antigen. Klotho protein expression was confirmed. This study confirmed that USCs from healthy and CKD dogs can act as stem cells, with those from sick dogs having greater proliferative ability with the potential for use as autologous therapies. |
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| id | doaj-art-07a20b71878c407aa8475e3043d073fb |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-07a20b71878c407aa8475e3043d073fb2025-01-24T13:18:15ZengMDPI AGAnimals2076-26152025-01-0115224210.3390/ani15020242Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD)Anna Lange-Consiglio0Filippo Tagliasacchi1Fausto Cremonesi2Claudia Gusmara3Claudia Pollera4Paola Scarpa5Giulia Gaspari6Pietro Riccaboni7Reproduction Laboratory, Department of Veterinary Medicine and Animal Science, Università degli Studi di Milano, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Science, Università degli Studi di Milano, 26900 Lodi, ItalyReproduction Laboratory, Department of Veterinary Medicine and Animal Science, Università degli Studi di Milano, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Science, Università degli Studi di Milano, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Science, Università degli Studi di Milano, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Science, Università degli Studi di Milano, 26900 Lodi, ItalyReproduction Laboratory, Department of Veterinary Medicine and Animal Science, Università degli Studi di Milano, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Science, Università degli Studi di Milano, 26900 Lodi, ItalyUrine-derived mesenchymal stromal/stem cells (USCs) could be a valuable source of cells in regenerative medicine because urine can be easily collected non-invasively. In this paper, USCs were isolated from both healthy dogs and dogs affected by chronic kidney disease (CKD), and the efficacy of collection methods (spontaneous micturition, bladder catheterization, and cystocentesis) were compared. Isolated cells were cultured in the presence of platelet-rich plasma and studied for their proliferative capacity (growth curve, doubling time, and colony forming unit), differentiation properties, expression of mesenchymal markers, and Klotho protein. Morphologically, all cells were elongated and fibroblast-like. USCs isolated from samples collected by spontaneous micturition and bladder catheterization failed to proliferate, whilst USCs obtained by cystocentesis showed a doubling time of 2.04 days in healthy dogs and 1.7 days in dogs with CKD (<i>p</i> < 0.05). Cells were able to differentiate into osteogenic, chondrogenic, and adipogenic lines, showed positive expression to mesenchymal/stem markers, negative expression to hematopoietic markers, and major histocompatibility complex (MHCII) antigen. Klotho protein expression was confirmed. This study confirmed that USCs from healthy and CKD dogs can act as stem cells, with those from sick dogs having greater proliferative ability with the potential for use as autologous therapies.https://www.mdpi.com/2076-2615/15/2/242dogurinemesenchymal/stem cellschronic kidney diseaseregenerative medicine |
| spellingShingle | Anna Lange-Consiglio Filippo Tagliasacchi Fausto Cremonesi Claudia Gusmara Claudia Pollera Paola Scarpa Giulia Gaspari Pietro Riccaboni Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD) Animals dog urine mesenchymal/stem cells chronic kidney disease regenerative medicine |
| title | Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD) |
| title_full | Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD) |
| title_fullStr | Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD) |
| title_full_unstemmed | Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD) |
| title_short | Characterization of Urine-Derived Stromal/Stem Cells from Healthy Dogs and Dogs Affected by Chronic Kidney Disease (CKD) |
| title_sort | characterization of urine derived stromal stem cells from healthy dogs and dogs affected by chronic kidney disease ckd |
| topic | dog urine mesenchymal/stem cells chronic kidney disease regenerative medicine |
| url | https://www.mdpi.com/2076-2615/15/2/242 |
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