MicroRNA-4735-3p Facilitates Ferroptosis in Clear Cell Renal Cell Carcinoma by Targeting SLC40A1
Objective. Clear cell renal cell carcinoma (ccRCC) is the major histopathological subtype of renal cancer, and ferroptosis is implicated in the pathogenesis of ccRCC. The present study was aimed at investigating the role and underlying mechanisms of microRNA-4735-3p (miR-4735-3p) in ccRCC. Methods....
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2022/4213401 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550881811234816 |
|---|---|
| author | Cairong Zhu Zhiquan Song Zhen Chen Tianqi Lin Haili Lin Zhenqiang Xu Fen Ai Shijiao Zheng |
| author_facet | Cairong Zhu Zhiquan Song Zhen Chen Tianqi Lin Haili Lin Zhenqiang Xu Fen Ai Shijiao Zheng |
| author_sort | Cairong Zhu |
| collection | DOAJ |
| description | Objective. Clear cell renal cell carcinoma (ccRCC) is the major histopathological subtype of renal cancer, and ferroptosis is implicated in the pathogenesis of ccRCC. The present study was aimed at investigating the role and underlying mechanisms of microRNA-4735-3p (miR-4735-3p) in ccRCC. Methods. Human ccRCC cell lines were transfected with the miR-4735-3p mimic or inhibitor to manipulate the expression of miR-4735-3p. Cell proliferation, colony formation, cell migration, cell invasion, cell death, oxidative stress, lipid peroxidation, and iron metabolism were determined. To validate the necessity of solute carrier family 40 member 1 (SLC40A1), human ccRCC cell lines were overexpressed with SLC40A1 using adenoviral vectors. Results. miR-4735-3p expression was reduced in human ccRCC tissues and cell lines but elevated upon ferroptotic stimulation. The miR-4735-3p mimic increased, while the miR-4735-3p inhibitor decreased oxidative stress, lipid peroxidation, iron overload, and ferroptosis of human ccRCC cell lines. Mechanistic studies identified SLC40A1 as a direct target of miR-4735-3p, and SLC40A1 overexpression significantly attenuated iron overload and ferroptosis in the miR-4735-3p mimic-treated human ccRCC cell lines. Conclusion. miR-4735-3p facilitates ferroptosis and tumor suppression in ccRCC by targeting SLC40A1. |
| format | Article |
| id | doaj-art-07a184a31c314206a980bbed9ef2c12e |
| institution | Kabale University |
| issn | 2210-7185 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-07a184a31c314206a980bbed9ef2c12e2025-02-03T06:05:25ZengWileyAnalytical Cellular Pathology2210-71852022-01-01202210.1155/2022/4213401MicroRNA-4735-3p Facilitates Ferroptosis in Clear Cell Renal Cell Carcinoma by Targeting SLC40A1Cairong Zhu0Zhiquan Song1Zhen Chen2Tianqi Lin3Haili Lin4Zhenqiang Xu5Fen Ai6Shijiao Zheng7Hemodialysis RoomDepartment of StomatologyDepartment of EmergencyDepartment of UrologyDepartment of UrologyDepartment of UrologyDepartment of EmergencyHemodialysis RoomObjective. Clear cell renal cell carcinoma (ccRCC) is the major histopathological subtype of renal cancer, and ferroptosis is implicated in the pathogenesis of ccRCC. The present study was aimed at investigating the role and underlying mechanisms of microRNA-4735-3p (miR-4735-3p) in ccRCC. Methods. Human ccRCC cell lines were transfected with the miR-4735-3p mimic or inhibitor to manipulate the expression of miR-4735-3p. Cell proliferation, colony formation, cell migration, cell invasion, cell death, oxidative stress, lipid peroxidation, and iron metabolism were determined. To validate the necessity of solute carrier family 40 member 1 (SLC40A1), human ccRCC cell lines were overexpressed with SLC40A1 using adenoviral vectors. Results. miR-4735-3p expression was reduced in human ccRCC tissues and cell lines but elevated upon ferroptotic stimulation. The miR-4735-3p mimic increased, while the miR-4735-3p inhibitor decreased oxidative stress, lipid peroxidation, iron overload, and ferroptosis of human ccRCC cell lines. Mechanistic studies identified SLC40A1 as a direct target of miR-4735-3p, and SLC40A1 overexpression significantly attenuated iron overload and ferroptosis in the miR-4735-3p mimic-treated human ccRCC cell lines. Conclusion. miR-4735-3p facilitates ferroptosis and tumor suppression in ccRCC by targeting SLC40A1.http://dx.doi.org/10.1155/2022/4213401 |
| spellingShingle | Cairong Zhu Zhiquan Song Zhen Chen Tianqi Lin Haili Lin Zhenqiang Xu Fen Ai Shijiao Zheng MicroRNA-4735-3p Facilitates Ferroptosis in Clear Cell Renal Cell Carcinoma by Targeting SLC40A1 Analytical Cellular Pathology |
| title | MicroRNA-4735-3p Facilitates Ferroptosis in Clear Cell Renal Cell Carcinoma by Targeting SLC40A1 |
| title_full | MicroRNA-4735-3p Facilitates Ferroptosis in Clear Cell Renal Cell Carcinoma by Targeting SLC40A1 |
| title_fullStr | MicroRNA-4735-3p Facilitates Ferroptosis in Clear Cell Renal Cell Carcinoma by Targeting SLC40A1 |
| title_full_unstemmed | MicroRNA-4735-3p Facilitates Ferroptosis in Clear Cell Renal Cell Carcinoma by Targeting SLC40A1 |
| title_short | MicroRNA-4735-3p Facilitates Ferroptosis in Clear Cell Renal Cell Carcinoma by Targeting SLC40A1 |
| title_sort | microrna 4735 3p facilitates ferroptosis in clear cell renal cell carcinoma by targeting slc40a1 |
| url | http://dx.doi.org/10.1155/2022/4213401 |
| work_keys_str_mv | AT cairongzhu microrna47353pfacilitatesferroptosisinclearcellrenalcellcarcinomabytargetingslc40a1 AT zhiquansong microrna47353pfacilitatesferroptosisinclearcellrenalcellcarcinomabytargetingslc40a1 AT zhenchen microrna47353pfacilitatesferroptosisinclearcellrenalcellcarcinomabytargetingslc40a1 AT tianqilin microrna47353pfacilitatesferroptosisinclearcellrenalcellcarcinomabytargetingslc40a1 AT haililin microrna47353pfacilitatesferroptosisinclearcellrenalcellcarcinomabytargetingslc40a1 AT zhenqiangxu microrna47353pfacilitatesferroptosisinclearcellrenalcellcarcinomabytargetingslc40a1 AT fenai microrna47353pfacilitatesferroptosisinclearcellrenalcellcarcinomabytargetingslc40a1 AT shijiaozheng microrna47353pfacilitatesferroptosisinclearcellrenalcellcarcinomabytargetingslc40a1 |