Muscle metabolism in response to oxidized fish oil feed in juvenile Nile tilapia
To investigate the effect of oxidized fish oil on fish muscle metabolic responses and flesh quality, Nile tilapia (Oreochromis niloticus) weighing 13.73 ± 0.31 g were fed two diets for 12 weeks: a fresh fish oil (FFO) and a highly oxidized fish oil (OFO) diet. The peroxide value of the FFO and OF...
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KeAi Communications Co. Ltd.
2025-01-01
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| Series: | Water Biology and Security |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772735124000933 |
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| author | Xinyuan Li Lili Chen Hua Wen Xuan Wang Di Peng Jianmin Zhang Yangyang Liu Ming Jiang Lixue Dong Feng Huang Juan Tian |
| author_facet | Xinyuan Li Lili Chen Hua Wen Xuan Wang Di Peng Jianmin Zhang Yangyang Liu Ming Jiang Lixue Dong Feng Huang Juan Tian |
| author_sort | Xinyuan Li |
| collection | DOAJ |
| description | To investigate the effect of oxidized fish oil on fish muscle metabolic responses and flesh quality, Nile tilapia (Oreochromis niloticus) weighing 13.73 ± 0.31 g were fed two diets for 12 weeks: a fresh fish oil (FFO) and a highly oxidized fish oil (OFO) diet. The peroxide value of the FFO and OFO diets was 2.2 meq/kg and 120.6 meq/kg, respectively. The OFO diet resulted in a decrease in growth, muscularity, nutritional value of fatty acids in the muscle, and density of myofibers. In the OFO group, the mRNA expression levels of lipolysis genes including lipoprotein lipase (lpl) and hormone-sensitive lipase (hsl) in the muscle were upregulated compared with the FFO group, while, the mRNA expression level of acetyl CoA carboxylase was downregulated. Additionally, under the positive ion mode, the levels of DHA deposition in phosphatidylcholine and phosphatidyl ethanolamine were reduced in the muscle of the OFO group compared to the FFO group. A total of 68 proteins were identified in the muscle, of which 42 were up-regulated and 26 were down-regulated. The KEGG pathway enrichment analysis revealed that the major pathways of the differentially abundant proteins were the Wnt signaling pathway, TGF-beta signaling pathway, and proteoglycans in cancer. In summary, the OFO diet negatively affected growth, muscularity, and nutritional value. It also inhibited the transformation of myofiber types and led to the apoptosis of myofibers. |
| format | Article |
| id | doaj-art-076f9cd9fd514507ba634a8eb0d95918 |
| institution | Kabale University |
| issn | 2772-7351 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co. Ltd. |
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| series | Water Biology and Security |
| spelling | doaj-art-076f9cd9fd514507ba634a8eb0d959182025-01-27T04:22:42ZengKeAi Communications Co. Ltd.Water Biology and Security2772-73512025-01-0141100321Muscle metabolism in response to oxidized fish oil feed in juvenile Nile tilapiaXinyuan Li0Lili Chen1Hua Wen2Xuan Wang3Di Peng4Jianmin Zhang5Yangyang Liu6Ming Jiang7Lixue Dong8Feng Huang9Juan Tian10Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, ChinaKey Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China; Corresponding author.Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China; Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China; Corresponding author. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, ChinaTo investigate the effect of oxidized fish oil on fish muscle metabolic responses and flesh quality, Nile tilapia (Oreochromis niloticus) weighing 13.73 ± 0.31 g were fed two diets for 12 weeks: a fresh fish oil (FFO) and a highly oxidized fish oil (OFO) diet. The peroxide value of the FFO and OFO diets was 2.2 meq/kg and 120.6 meq/kg, respectively. The OFO diet resulted in a decrease in growth, muscularity, nutritional value of fatty acids in the muscle, and density of myofibers. In the OFO group, the mRNA expression levels of lipolysis genes including lipoprotein lipase (lpl) and hormone-sensitive lipase (hsl) in the muscle were upregulated compared with the FFO group, while, the mRNA expression level of acetyl CoA carboxylase was downregulated. Additionally, under the positive ion mode, the levels of DHA deposition in phosphatidylcholine and phosphatidyl ethanolamine were reduced in the muscle of the OFO group compared to the FFO group. A total of 68 proteins were identified in the muscle, of which 42 were up-regulated and 26 were down-regulated. The KEGG pathway enrichment analysis revealed that the major pathways of the differentially abundant proteins were the Wnt signaling pathway, TGF-beta signaling pathway, and proteoglycans in cancer. In summary, the OFO diet negatively affected growth, muscularity, and nutritional value. It also inhibited the transformation of myofiber types and led to the apoptosis of myofibers.http://www.sciencedirect.com/science/article/pii/S2772735124000933Oxidized fish oilNile tilapiaMyofiberLipidomicsProteomics |
| spellingShingle | Xinyuan Li Lili Chen Hua Wen Xuan Wang Di Peng Jianmin Zhang Yangyang Liu Ming Jiang Lixue Dong Feng Huang Juan Tian Muscle metabolism in response to oxidized fish oil feed in juvenile Nile tilapia Water Biology and Security Oxidized fish oil Nile tilapia Myofiber Lipidomics Proteomics |
| title | Muscle metabolism in response to oxidized fish oil feed in juvenile Nile tilapia |
| title_full | Muscle metabolism in response to oxidized fish oil feed in juvenile Nile tilapia |
| title_fullStr | Muscle metabolism in response to oxidized fish oil feed in juvenile Nile tilapia |
| title_full_unstemmed | Muscle metabolism in response to oxidized fish oil feed in juvenile Nile tilapia |
| title_short | Muscle metabolism in response to oxidized fish oil feed in juvenile Nile tilapia |
| title_sort | muscle metabolism in response to oxidized fish oil feed in juvenile nile tilapia |
| topic | Oxidized fish oil Nile tilapia Myofiber Lipidomics Proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S2772735124000933 |
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