Characterization and evaluation of antioxidant properties of leaf extracts from Rhododendron arboreum
Phytochemicals have been known to suppress the microbial activity and oxidative stress, which indicates the significance of plant material as anti-microbial and antioxidant agent. In this light the current study is worked out to screen the phytochemicals from the Rhododendron arboretum leaf extract...
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| Format: | Article |
| Language: | English |
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Plovdiv University Press
2025-01-01
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| Series: | Journal of BioScience and Biotechnology |
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| Online Access: | https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/545/392 |
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| author | Shobha Mehra Ufondu Maryann Afoma Namrata Dudha Shilpy Singh Shweta Sharma Ved Vrat Verma Mohammad Zuhaib Varun Kumar Sharma |
| author_facet | Shobha Mehra Ufondu Maryann Afoma Namrata Dudha Shilpy Singh Shweta Sharma Ved Vrat Verma Mohammad Zuhaib Varun Kumar Sharma |
| author_sort | Shobha Mehra |
| collection | DOAJ |
| description | Phytochemicals have been known to suppress the microbial activity and oxidative stress, which indicates the significance of plant material as anti-microbial and antioxidant agent. In this light the current study is worked out to screen the phytochemicals from the Rhododendron arboretum leaf extract and characterize it using HPLC. The alkaloids and flavonoids are the major secondary metabolites found during screening. Further, anti-oxidant properties of leaf extract of R. arboreum prepared in different solvent (methanol, ethyl acetate, petroleum ether, and chloroform) were analyzed using DPPH and FRAP assay. Observation of the present study indicates that methanolic extracts was found to show significant anti-oxidant activity with the IC50 value of 241.2 µg/ml and 163.6 µg/ml in DPPH and FRAP assay respectively. In conclusion, the leaf extracts of R. arboretum can be one of the better natural source of antioxidant source and could be used for therapeutic purpose in the treatments of different diseases. |
| format | Article |
| id | doaj-art-073f5cd7ad9743618afeea94e38e4923 |
| institution | Kabale University |
| issn | 1314-6238 1314-6246 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Plovdiv University Press |
| record_format | Article |
| series | Journal of BioScience and Biotechnology |
| spelling | doaj-art-073f5cd7ad9743618afeea94e38e49232025-01-28T07:35:00ZengPlovdiv University PressJournal of BioScience and Biotechnology1314-62381314-62462025-01-0113214715310.69085/jbb20242147Characterization and evaluation of antioxidant properties of leaf extracts from Rhododendron arboreumShobha Mehra0Ufondu Maryann Afoma1Namrata Dudha2Shilpy Singh3Shweta Sharma4Ved Vrat Verma5Mohammad Zuhaib6Varun Kumar Sharma7Department of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, IndiaDepartment of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, IndiaDepartment of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, IndiaDepartment of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, IndiaDepartment of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, IndiaDepartment of Biotechnology, School of Engineering and Technology, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, IndiaDepartment of Agriculture, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, IndiaDepartment of Biotechnology & Microbiology, School of Sciences, Noida International University-NIU, Gautam Budh Nagar-201308, Uttar Pradesh, IndiaPhytochemicals have been known to suppress the microbial activity and oxidative stress, which indicates the significance of plant material as anti-microbial and antioxidant agent. In this light the current study is worked out to screen the phytochemicals from the Rhododendron arboretum leaf extract and characterize it using HPLC. The alkaloids and flavonoids are the major secondary metabolites found during screening. Further, anti-oxidant properties of leaf extract of R. arboreum prepared in different solvent (methanol, ethyl acetate, petroleum ether, and chloroform) were analyzed using DPPH and FRAP assay. Observation of the present study indicates that methanolic extracts was found to show significant anti-oxidant activity with the IC50 value of 241.2 µg/ml and 163.6 µg/ml in DPPH and FRAP assay respectively. In conclusion, the leaf extracts of R. arboretum can be one of the better natural source of antioxidant source and could be used for therapeutic purpose in the treatments of different diseases.https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/545/392phytochemicalsecondary metaboliterhodendron arboreumhplcantioxidant activity |
| spellingShingle | Shobha Mehra Ufondu Maryann Afoma Namrata Dudha Shilpy Singh Shweta Sharma Ved Vrat Verma Mohammad Zuhaib Varun Kumar Sharma Characterization and evaluation of antioxidant properties of leaf extracts from Rhododendron arboreum Journal of BioScience and Biotechnology phytochemical secondary metabolite rhodendron arboreum hplc antioxidant activity |
| title | Characterization and evaluation of antioxidant properties of leaf extracts from Rhododendron arboreum |
| title_full | Characterization and evaluation of antioxidant properties of leaf extracts from Rhododendron arboreum |
| title_fullStr | Characterization and evaluation of antioxidant properties of leaf extracts from Rhododendron arboreum |
| title_full_unstemmed | Characterization and evaluation of antioxidant properties of leaf extracts from Rhododendron arboreum |
| title_short | Characterization and evaluation of antioxidant properties of leaf extracts from Rhododendron arboreum |
| title_sort | characterization and evaluation of antioxidant properties of leaf extracts from rhododendron arboreum |
| topic | phytochemical secondary metabolite rhodendron arboreum hplc antioxidant activity |
| url | https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/545/392 |
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