Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver
Rheumatoid arthritis (RA) is a chronic inflammatory disease, leading to progressive destruction of joints and extra-articular tissues, including organs such as liver and spleen. The purpose of this study was to compare the effects of a potential immunomodulator, natural polyphenol N-feruloylserotoni...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/7509653 |
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| author | Ľudmila Pašková Viera Kuncírová Silvester Poništ Danica Mihálová Radomír Nosáľ Juraj Harmatha Iveta Hrádková Tomáš Čavojský František Bilka Katarína Šišková Ingrid Paulíková Lýdia Bezáková Katarína Bauerová |
| author_facet | Ľudmila Pašková Viera Kuncírová Silvester Poništ Danica Mihálová Radomír Nosáľ Juraj Harmatha Iveta Hrádková Tomáš Čavojský František Bilka Katarína Šišková Ingrid Paulíková Lýdia Bezáková Katarína Bauerová |
| author_sort | Ľudmila Pašková |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) is a chronic inflammatory disease, leading to progressive destruction of joints and extra-articular tissues, including organs such as liver and spleen. The purpose of this study was to compare the effects of a potential immunomodulator, natural polyphenol N-feruloylserotonin (N-f-5HT), with methotrexate (MTX), the standard in RA therapy, in the chronic phase of adjuvant-induced arthritis (AA) in male Lewis rats. The experiment included healthy controls (CO), arthritic animals (AA), AA given N-f-5HT (AA-N-f-5HT), and AA given MTX (AA-MTX). N-f-5HT did not affect the body weight change and clinical parameters until the 14th experimental day. Its positive effect was rising during the 28-day experiment, indicating a delayed onset of N-f-5HT action. Administration of either N-f-5HT or MTX caused reduction of inflammation measured as the level of CRP in plasma and the activity of LOX in the liver. mRNA transcription of TNF-α and iNOS in the liver was significantly attenuated in both MTX and N-f-5HT treated groups of arthritic rats. Interestingly, in contrast to MTX, N-f-5HT significantly lowered the level of IL-1β in plasma and IL-1β mRNA expression in the liver and spleen of arthritic rats. This speaks for future investigations of N-f-5HT as an agent in the treatment of RA in combination therapy with MTX. |
| format | Article |
| id | doaj-art-072222128a704d429b0ad2a1c2b52eef |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-072222128a704d429b0ad2a1c2b52eef2025-02-03T05:45:57ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/75096537509653Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in LiverĽudmila Pašková0Viera Kuncírová1Silvester Poništ2Danica Mihálová3Radomír Nosáľ4Juraj Harmatha5Iveta Hrádková6Tomáš Čavojský7František Bilka8Katarína Šišková9Ingrid Paulíková10Lýdia Bezáková11Katarína Bauerová12Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, SlovakiaInstitute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, 841 04 Bratislava, SlovakiaInstitute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, 841 04 Bratislava, SlovakiaInstitute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, 841 04 Bratislava, SlovakiaInstitute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, 841 04 Bratislava, SlovakiaInstitute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 166 10 Prague, Czech RepublicFaculty of Food and Biochemical Technology, University of Chemistry and Technology, 166 28 Prague, Czech RepublicDepartment of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, SlovakiaDepartment of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, SlovakiaDepartment of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, SlovakiaDepartment of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, SlovakiaDepartment of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, 832 32 Bratislava, SlovakiaInstitute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, 841 04 Bratislava, SlovakiaRheumatoid arthritis (RA) is a chronic inflammatory disease, leading to progressive destruction of joints and extra-articular tissues, including organs such as liver and spleen. The purpose of this study was to compare the effects of a potential immunomodulator, natural polyphenol N-feruloylserotonin (N-f-5HT), with methotrexate (MTX), the standard in RA therapy, in the chronic phase of adjuvant-induced arthritis (AA) in male Lewis rats. The experiment included healthy controls (CO), arthritic animals (AA), AA given N-f-5HT (AA-N-f-5HT), and AA given MTX (AA-MTX). N-f-5HT did not affect the body weight change and clinical parameters until the 14th experimental day. Its positive effect was rising during the 28-day experiment, indicating a delayed onset of N-f-5HT action. Administration of either N-f-5HT or MTX caused reduction of inflammation measured as the level of CRP in plasma and the activity of LOX in the liver. mRNA transcription of TNF-α and iNOS in the liver was significantly attenuated in both MTX and N-f-5HT treated groups of arthritic rats. Interestingly, in contrast to MTX, N-f-5HT significantly lowered the level of IL-1β in plasma and IL-1β mRNA expression in the liver and spleen of arthritic rats. This speaks for future investigations of N-f-5HT as an agent in the treatment of RA in combination therapy with MTX.http://dx.doi.org/10.1155/2016/7509653 |
| spellingShingle | Ľudmila Pašková Viera Kuncírová Silvester Poništ Danica Mihálová Radomír Nosáľ Juraj Harmatha Iveta Hrádková Tomáš Čavojský František Bilka Katarína Šišková Ingrid Paulíková Lýdia Bezáková Katarína Bauerová Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver Journal of Immunology Research |
| title | Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver |
| title_full | Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver |
| title_fullStr | Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver |
| title_full_unstemmed | Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver |
| title_short | Effect of N-Feruloylserotonin and Methotrexate on Severity of Experimental Arthritis and on Messenger RNA Expression of Key Proinflammatory Markers in Liver |
| title_sort | effect of n feruloylserotonin and methotrexate on severity of experimental arthritis and on messenger rna expression of key proinflammatory markers in liver |
| url | http://dx.doi.org/10.1155/2016/7509653 |
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