A New Murine Model of Chronic Kidney Disease-Mineral and Bone Disorder
Chronic kidney disease (CKD) is associated with mineral and bone disorder (MBD), which is the main cause of the extensively increased cardiovascular mortality in the CKD population. We now aimed to establish a new murine experimental CKD-MBD model. Dilute brown non-Agouti (DBA/2) mice were fed with...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2017/1659071 |
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| author | Bianca Frauscher Katharina Artinger Alexander H. Kirsch Ida Aringer Foteini Moschovaki-Filippidou Máté Kétszeri Corinna Schabhüttl Peter P. Rainer Albrecht Schmidt Tatjana Stojakovic Astrid Fahrleitner-Pammer Alexander R. Rosenkranz Philipp Eller Kathrin Eller |
| author_facet | Bianca Frauscher Katharina Artinger Alexander H. Kirsch Ida Aringer Foteini Moschovaki-Filippidou Máté Kétszeri Corinna Schabhüttl Peter P. Rainer Albrecht Schmidt Tatjana Stojakovic Astrid Fahrleitner-Pammer Alexander R. Rosenkranz Philipp Eller Kathrin Eller |
| author_sort | Bianca Frauscher |
| collection | DOAJ |
| description | Chronic kidney disease (CKD) is associated with mineral and bone disorder (MBD), which is the main cause of the extensively increased cardiovascular mortality in the CKD population. We now aimed to establish a new murine experimental CKD-MBD model. Dilute brown non-Agouti (DBA/2) mice were fed with high-phosphate diet for 4 (HPD4) or 7 (HPD7) days, then with standard chow diet (SCD) and subsequently followed until day 84. They were compared to DBA/2 mice maintained on SCD during the whole study period. Both 4 and 7 days HPD-fed mice developed phosphate nephropathy with tubular atrophy, interstitial fibrosis, decreased glomerular filtration rate, and increased serum urea levels. The abdominal aorta of HPD-treated mice showed signs of media calcification. Histomorphometric analysis of HPD-treated mice showed decreased bone volume/tissue volume, low mineral apposition rate, and low bone formation rate as compared to SCD-fed mice, despite increased parathyroid hormone levels. Overall, the observed phenotype was more pronounced in the HPD7 group. In summary, we established a new, noninvasive, and therefore easy to perform reproducible CKD-MBD model, which showed media calcification, secondary hyperparathyroidism, and low-turnover bone disease. |
| format | Article |
| id | doaj-art-06f911eaebaf4b73b7e74ffeb68a71f0 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-06f911eaebaf4b73b7e74ffeb68a71f02025-02-03T06:08:29ZengWileyInternational Journal of Endocrinology1687-83371687-83452017-01-01201710.1155/2017/16590711659071A New Murine Model of Chronic Kidney Disease-Mineral and Bone DisorderBianca Frauscher0Katharina Artinger1Alexander H. Kirsch2Ida Aringer3Foteini Moschovaki-Filippidou4Máté Kétszeri5Corinna Schabhüttl6Peter P. Rainer7Albrecht Schmidt8Tatjana Stojakovic9Astrid Fahrleitner-Pammer10Alexander R. Rosenkranz11Philipp Eller12Kathrin Eller13Clinical Division of Nephrology, Medical University of Graz, Graz, AustriaClinical Division of Nephrology, Medical University of Graz, Graz, AustriaClinical Division of Nephrology, Medical University of Graz, Graz, AustriaClinical Division of Nephrology, Medical University of Graz, Graz, AustriaClinical Division of Nephrology, Medical University of Graz, Graz, AustriaClinical Division of Nephrology, Medical University of Graz, Graz, AustriaClinical Division of Nephrology, Medical University of Graz, Graz, AustriaClinical Division of Cardiology, Medical University of Graz, Graz, AustriaClinical Division of Cardiology, Medical University of Graz, Graz, AustriaClinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, AustriaClinical Division of Endocrinology and Diabetes, Medical University of Graz, Graz, AustriaClinical Division of Nephrology, Medical University of Graz, Graz, AustriaIntensive Care Unit, Department of Internal Medicine, Medical University of Graz, Graz, AustriaClinical Division of Nephrology, Medical University of Graz, Graz, AustriaChronic kidney disease (CKD) is associated with mineral and bone disorder (MBD), which is the main cause of the extensively increased cardiovascular mortality in the CKD population. We now aimed to establish a new murine experimental CKD-MBD model. Dilute brown non-Agouti (DBA/2) mice were fed with high-phosphate diet for 4 (HPD4) or 7 (HPD7) days, then with standard chow diet (SCD) and subsequently followed until day 84. They were compared to DBA/2 mice maintained on SCD during the whole study period. Both 4 and 7 days HPD-fed mice developed phosphate nephropathy with tubular atrophy, interstitial fibrosis, decreased glomerular filtration rate, and increased serum urea levels. The abdominal aorta of HPD-treated mice showed signs of media calcification. Histomorphometric analysis of HPD-treated mice showed decreased bone volume/tissue volume, low mineral apposition rate, and low bone formation rate as compared to SCD-fed mice, despite increased parathyroid hormone levels. Overall, the observed phenotype was more pronounced in the HPD7 group. In summary, we established a new, noninvasive, and therefore easy to perform reproducible CKD-MBD model, which showed media calcification, secondary hyperparathyroidism, and low-turnover bone disease.http://dx.doi.org/10.1155/2017/1659071 |
| spellingShingle | Bianca Frauscher Katharina Artinger Alexander H. Kirsch Ida Aringer Foteini Moschovaki-Filippidou Máté Kétszeri Corinna Schabhüttl Peter P. Rainer Albrecht Schmidt Tatjana Stojakovic Astrid Fahrleitner-Pammer Alexander R. Rosenkranz Philipp Eller Kathrin Eller A New Murine Model of Chronic Kidney Disease-Mineral and Bone Disorder International Journal of Endocrinology |
| title | A New Murine Model of Chronic Kidney Disease-Mineral and Bone Disorder |
| title_full | A New Murine Model of Chronic Kidney Disease-Mineral and Bone Disorder |
| title_fullStr | A New Murine Model of Chronic Kidney Disease-Mineral and Bone Disorder |
| title_full_unstemmed | A New Murine Model of Chronic Kidney Disease-Mineral and Bone Disorder |
| title_short | A New Murine Model of Chronic Kidney Disease-Mineral and Bone Disorder |
| title_sort | new murine model of chronic kidney disease mineral and bone disorder |
| url | http://dx.doi.org/10.1155/2017/1659071 |
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