Progenitor Hematopoietic Cells Implantation Improves Functional Capacity of End Stage Coronary Artery Disease Patients with Advanced Heart Failure
Background. Proangiogenic Hematopoietic Cells (PHC) which comprise diverse mixture of cell types are able to secrete proangiogenic factors and interesting candidate for cell therapy. The aim of this study was to seek for benefit in implantation of PHC on functional improvement in end stage coronary...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Cardiology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2016/3942605 |
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| author | Yoga Yuniadi Yuyus Kusnadi Lakshmi Sandhow Rendra Erika Dicky A. Hanafy Caroline Sardjono R. W. M. Kaligis Manoefris Kasim Ganesja M. Harimurti |
| author_facet | Yoga Yuniadi Yuyus Kusnadi Lakshmi Sandhow Rendra Erika Dicky A. Hanafy Caroline Sardjono R. W. M. Kaligis Manoefris Kasim Ganesja M. Harimurti |
| author_sort | Yoga Yuniadi |
| collection | DOAJ |
| description | Background. Proangiogenic Hematopoietic Cells (PHC) which comprise diverse mixture of cell types are able to secrete proangiogenic factors and interesting candidate for cell therapy. The aim of this study was to seek for benefit in implantation of PHC on functional improvement in end stage coronary artery disease patients with advanced heart failure. Methods. Patients with symptomatic heart failure despite guideline directed medical therapy and LVEF less than 35% were included. Peripheral blood mononuclear cells were isolated, cultivated for 5 days, and then harvested. Flow cytometry and cell surface markers were used to characterize PHC. The PHC were delivered retrogradely via sinus coronarius. Echocardiography, myocardial perfusion, and clinical and functional data were analyzed up to 1-year observation. Results. Of 30 patients (56.4±7.40 yo) preimplant NT proBNP level is 5124.5±4682.50 pmol/L. Harvested cells characterized with CD133, CD34, CD45, and KDR showed 0.87±0.41, 0.63±0.66, 99.00±2.60, and 3.22±3.79%, respectively. LVEF was improved (22±5.68 versus 26.8±7.93, p<0.001) during short and long term observation. Myocardial perfusion significantly improved 6 months after treatment. NYHA Class and six-minute walk test are improved during short term and long term follow-up. Conclusion. Expanded peripheral blood PHC implantation using retrograde delivery approach improved LV systolic function, myocardial perfusion, and functional capacity. |
| format | Article |
| id | doaj-art-06f6555ab4d04a2d9d78cc52980cc1fa |
| institution | Kabale University |
| issn | 2090-8016 2090-0597 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cardiology Research and Practice |
| spelling | doaj-art-06f6555ab4d04a2d9d78cc52980cc1fa2025-02-03T01:29:15ZengWileyCardiology Research and Practice2090-80162090-05972016-01-01201610.1155/2016/39426053942605Progenitor Hematopoietic Cells Implantation Improves Functional Capacity of End Stage Coronary Artery Disease Patients with Advanced Heart FailureYoga Yuniadi0Yuyus Kusnadi1Lakshmi Sandhow2Rendra Erika3Dicky A. Hanafy4Caroline Sardjono5R. W. M. Kaligis6Manoefris Kasim7Ganesja M. Harimurti8Department of Cardiology and Vascular Medicine, Faculty of Medicine, University of Indonesia and National Cardiovascular Center Harapan Kita, Jakarta 11420, IndonesiaStem Cell and Cancer Institute, Jakarta 13210, IndonesiaStem Cell and Cancer Institute, Jakarta 13210, IndonesiaStem Cell and Cancer Institute, Jakarta 13210, IndonesiaDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, University of Indonesia and National Cardiovascular Center Harapan Kita, Jakarta 11420, IndonesiaStem Cell and Cancer Institute, Jakarta 13210, IndonesiaDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, University of Indonesia and National Cardiovascular Center Harapan Kita, Jakarta 11420, IndonesiaDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, University of Indonesia and National Cardiovascular Center Harapan Kita, Jakarta 11420, IndonesiaDepartment of Cardiology and Vascular Medicine, Faculty of Medicine, University of Indonesia and National Cardiovascular Center Harapan Kita, Jakarta 11420, IndonesiaBackground. Proangiogenic Hematopoietic Cells (PHC) which comprise diverse mixture of cell types are able to secrete proangiogenic factors and interesting candidate for cell therapy. The aim of this study was to seek for benefit in implantation of PHC on functional improvement in end stage coronary artery disease patients with advanced heart failure. Methods. Patients with symptomatic heart failure despite guideline directed medical therapy and LVEF less than 35% were included. Peripheral blood mononuclear cells were isolated, cultivated for 5 days, and then harvested. Flow cytometry and cell surface markers were used to characterize PHC. The PHC were delivered retrogradely via sinus coronarius. Echocardiography, myocardial perfusion, and clinical and functional data were analyzed up to 1-year observation. Results. Of 30 patients (56.4±7.40 yo) preimplant NT proBNP level is 5124.5±4682.50 pmol/L. Harvested cells characterized with CD133, CD34, CD45, and KDR showed 0.87±0.41, 0.63±0.66, 99.00±2.60, and 3.22±3.79%, respectively. LVEF was improved (22±5.68 versus 26.8±7.93, p<0.001) during short and long term observation. Myocardial perfusion significantly improved 6 months after treatment. NYHA Class and six-minute walk test are improved during short term and long term follow-up. Conclusion. Expanded peripheral blood PHC implantation using retrograde delivery approach improved LV systolic function, myocardial perfusion, and functional capacity.http://dx.doi.org/10.1155/2016/3942605 |
| spellingShingle | Yoga Yuniadi Yuyus Kusnadi Lakshmi Sandhow Rendra Erika Dicky A. Hanafy Caroline Sardjono R. W. M. Kaligis Manoefris Kasim Ganesja M. Harimurti Progenitor Hematopoietic Cells Implantation Improves Functional Capacity of End Stage Coronary Artery Disease Patients with Advanced Heart Failure Cardiology Research and Practice |
| title | Progenitor Hematopoietic Cells Implantation Improves Functional Capacity of End Stage Coronary Artery Disease Patients with Advanced Heart Failure |
| title_full | Progenitor Hematopoietic Cells Implantation Improves Functional Capacity of End Stage Coronary Artery Disease Patients with Advanced Heart Failure |
| title_fullStr | Progenitor Hematopoietic Cells Implantation Improves Functional Capacity of End Stage Coronary Artery Disease Patients with Advanced Heart Failure |
| title_full_unstemmed | Progenitor Hematopoietic Cells Implantation Improves Functional Capacity of End Stage Coronary Artery Disease Patients with Advanced Heart Failure |
| title_short | Progenitor Hematopoietic Cells Implantation Improves Functional Capacity of End Stage Coronary Artery Disease Patients with Advanced Heart Failure |
| title_sort | progenitor hematopoietic cells implantation improves functional capacity of end stage coronary artery disease patients with advanced heart failure |
| url | http://dx.doi.org/10.1155/2016/3942605 |
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