Genotyping and drug susceptibility profiling of Prototheca sp. strains isolated from cases of protothecosis in dogs
Abstract Background Protothecosis in dogs is a rare, yet emerging disease, distinguished by its often‐aggressive clinical course and high fatality rate. Our study was conducted to enhance treatment protocols for affected dogs by better understanding the genetic diversity and drug resistance patterns...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Journal of Veterinary Internal Medicine |
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| Online Access: | https://doi.org/10.1111/jvim.17173 |
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| author | Angelika Proskurnicka Mateusz Iskra Sylwia Wronka Zofia Bakuła Patrizia Danesi Marconi Rodrigues deFarias Fábio Vinícius Ramos Portilho Márcio Garcia Ribeiro Uwe Rösler Rui Kano Richard Malik Tomasz Jagielski |
| author_facet | Angelika Proskurnicka Mateusz Iskra Sylwia Wronka Zofia Bakuła Patrizia Danesi Marconi Rodrigues deFarias Fábio Vinícius Ramos Portilho Márcio Garcia Ribeiro Uwe Rösler Rui Kano Richard Malik Tomasz Jagielski |
| author_sort | Angelika Proskurnicka |
| collection | DOAJ |
| description | Abstract Background Protothecosis in dogs is a rare, yet emerging disease, distinguished by its often‐aggressive clinical course and high fatality rate. Our study was conducted to enhance treatment protocols for affected dogs by better understanding the genetic diversity and drug resistance patterns of Prototheca species. Objectives To identify species and drug susceptibility profiles of an international collection of 28 Prototheca strains isolated from cases of protothecosis in dogs. Animals None. Methods Retrospective study. Species‐level identification was made for isolates from 28 dogs in 6 countries by molecular typing with the partial cytb gene as a marker. For the determination of minimum inhibitory concentrations (MICs) and minimum algicidal concentrations (MACs), the Clinical Laboratory Standards Institute (CLSI) protocol (M27‐A3) was used. Results Prototheca bovis was the most prevalent species, accounting for 75% (21/28) of the cases, followed by P. wickerhamii (18%; 5/28) and P. ciferrii (7%; 2/28). Of the 6 drugs tested, efinaconazole (EFZ) was the most potent in vitro, with its median MIC and MAC values equal to 0.125 mg/L. The lowest activity was found for fluconazole (FLU), with MIC and MAC medians of 48 mg/L and 64 mg/L, respectively. Conclusions and Clinical Importance Our study identifies P. bovis as the species that most frequently causes protothecosis in dogs, which suggests the possibility of cross‐species infection from other animals, especially cows. Additionally, it indicates that EFZ could be used in the treatment of infection in the colon. |
| format | Article |
| id | doaj-art-06d7806fa6d841ebb645adb336132e20 |
| institution | Kabale University |
| issn | 0891-6640 1939-1676 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj-art-06d7806fa6d841ebb645adb336132e202025-01-27T15:22:40ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762025-01-01391n/an/a10.1111/jvim.17173Genotyping and drug susceptibility profiling of Prototheca sp. strains isolated from cases of protothecosis in dogsAngelika Proskurnicka0Mateusz Iskra1Sylwia Wronka2Zofia Bakuła3Patrizia Danesi4Marconi Rodrigues deFarias5Fábio Vinícius Ramos Portilho6Márcio Garcia Ribeiro7Uwe Rösler8Rui Kano9Richard Malik10Tomasz Jagielski11Department of Medical Microbiology, Institute of Microbiology, Faculty of Biology University of Warsaw Warsaw PolandDepartment of Medical Microbiology, Institute of Microbiology, Faculty of Biology University of Warsaw Warsaw PolandDepartment of Medical Microbiology, Institute of Microbiology, Faculty of Biology University of Warsaw Warsaw PolandDepartment of Medical Microbiology, Institute of Microbiology, Faculty of Biology University of Warsaw Warsaw PolandIstituto Zooprofilattico Sperimentale delle Venezie Padua ItalyPostgraduate Program in Animal Science Pontifical Catholic University of Paraná Curitiba BrazilDepartment of Animal Production and Preventive Veterinary Medicine São Paulo State University Botucatu BrazilDepartment of Animal Production and Preventive Veterinary Medicine São Paulo State University Botucatu BrazilInstitute for Animal Hygiene and Environmental Health Freie Universitaet Berlin Berlin GermanyDepartment of Veterinary Dermatology Nihon University School of Veterinary Medicine Fujisawa Kanagawa JapanCentre for Veterinary Education, Sydney School of Veterinary Science The University of Sydney Sydney AustraliaDepartment of Medical Microbiology, Institute of Microbiology, Faculty of Biology University of Warsaw Warsaw PolandAbstract Background Protothecosis in dogs is a rare, yet emerging disease, distinguished by its often‐aggressive clinical course and high fatality rate. Our study was conducted to enhance treatment protocols for affected dogs by better understanding the genetic diversity and drug resistance patterns of Prototheca species. Objectives To identify species and drug susceptibility profiles of an international collection of 28 Prototheca strains isolated from cases of protothecosis in dogs. Animals None. Methods Retrospective study. Species‐level identification was made for isolates from 28 dogs in 6 countries by molecular typing with the partial cytb gene as a marker. For the determination of minimum inhibitory concentrations (MICs) and minimum algicidal concentrations (MACs), the Clinical Laboratory Standards Institute (CLSI) protocol (M27‐A3) was used. Results Prototheca bovis was the most prevalent species, accounting for 75% (21/28) of the cases, followed by P. wickerhamii (18%; 5/28) and P. ciferrii (7%; 2/28). Of the 6 drugs tested, efinaconazole (EFZ) was the most potent in vitro, with its median MIC and MAC values equal to 0.125 mg/L. The lowest activity was found for fluconazole (FLU), with MIC and MAC medians of 48 mg/L and 64 mg/L, respectively. Conclusions and Clinical Importance Our study identifies P. bovis as the species that most frequently causes protothecosis in dogs, which suggests the possibility of cross‐species infection from other animals, especially cows. Additionally, it indicates that EFZ could be used in the treatment of infection in the colon.https://doi.org/10.1111/jvim.17173algaecolitiscytbdogPrototheca spp.systemic infection |
| spellingShingle | Angelika Proskurnicka Mateusz Iskra Sylwia Wronka Zofia Bakuła Patrizia Danesi Marconi Rodrigues deFarias Fábio Vinícius Ramos Portilho Márcio Garcia Ribeiro Uwe Rösler Rui Kano Richard Malik Tomasz Jagielski Genotyping and drug susceptibility profiling of Prototheca sp. strains isolated from cases of protothecosis in dogs Journal of Veterinary Internal Medicine algae colitis cytb dog Prototheca spp. systemic infection |
| title | Genotyping and drug susceptibility profiling of Prototheca sp. strains isolated from cases of protothecosis in dogs |
| title_full | Genotyping and drug susceptibility profiling of Prototheca sp. strains isolated from cases of protothecosis in dogs |
| title_fullStr | Genotyping and drug susceptibility profiling of Prototheca sp. strains isolated from cases of protothecosis in dogs |
| title_full_unstemmed | Genotyping and drug susceptibility profiling of Prototheca sp. strains isolated from cases of protothecosis in dogs |
| title_short | Genotyping and drug susceptibility profiling of Prototheca sp. strains isolated from cases of protothecosis in dogs |
| title_sort | genotyping and drug susceptibility profiling of prototheca sp strains isolated from cases of protothecosis in dogs |
| topic | algae colitis cytb dog Prototheca spp. systemic infection |
| url | https://doi.org/10.1111/jvim.17173 |
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