Physiopathological effects of entrance versus distal spread-out Bragg peak on mouse spinal cord neurons
Abstract Recent investigations into radiation-induced side effects have focused on understanding the physiopathological consequences of irradiation on late-responding tissues like the spinal cord, which can lead to chronic progressive myelopathy. Proton therapy, an advanced radiation treatment, aims...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-84902-2 |
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| author | Filippo Torrisi Francesco Paolo Cammarata Valentina Bravatà Marco Calvaruso Cristiana Alberghina Anna Maria Pavone Giada Petringa Giuseppe Antonio Pablo Cirrone Simona Denaro Anna Gervasi Simona D’Aprile Nunzio Vicario Emanuele Scifoni Pietro Pisciotta Giorgio Russo Rosalba Parenti |
| author_facet | Filippo Torrisi Francesco Paolo Cammarata Valentina Bravatà Marco Calvaruso Cristiana Alberghina Anna Maria Pavone Giada Petringa Giuseppe Antonio Pablo Cirrone Simona Denaro Anna Gervasi Simona D’Aprile Nunzio Vicario Emanuele Scifoni Pietro Pisciotta Giorgio Russo Rosalba Parenti |
| author_sort | Filippo Torrisi |
| collection | DOAJ |
| description | Abstract Recent investigations into radiation-induced side effects have focused on understanding the physiopathological consequences of irradiation on late-responding tissues like the spinal cord, which can lead to chronic progressive myelopathy. Proton therapy, an advanced radiation treatment, aims to minimize damage to healthy tissues through precise dose deposition. However, challenges remain, particularly regarding the variation in dose distribution, characterized by maximum deposition at the end of the proton range, known as the distal fall-off of a spread-out Bragg peak. This variation is critical for nearby organs at risk. In this preliminary study, we evaluated the effects of proton irradiation on the neuronal cell population in mouse spinal cord by comparing two positions of the particle range dose deposition profile. We irradiated the spinal cords at the entrance and the distal edge of the spread-out Bragg peak with a single proton dose. Results showed changes in the expression of synaptophysin, a presynaptic protein crucial for synaptic plasticity. Our findings suggest that examining early radiation-induced physiopathological effects on late-responding tissues can provide valuable insights into neuronal plasticity, informing clinical treatment planning for proton beam positioning. |
| format | Article |
| id | doaj-art-06ae69d0a4094182b53b21080cf9ac65 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-06ae69d0a4094182b53b21080cf9ac652025-01-19T12:21:31ZengNature PortfolioScientific Reports2045-23222025-01-0115111110.1038/s41598-024-84902-2Physiopathological effects of entrance versus distal spread-out Bragg peak on mouse spinal cord neuronsFilippo Torrisi0Francesco Paolo Cammarata1Valentina Bravatà2Marco Calvaruso3Cristiana Alberghina4Anna Maria Pavone5Giada Petringa6Giuseppe Antonio Pablo Cirrone7Simona Denaro8Anna Gervasi9Simona D’Aprile10Nunzio Vicario11Emanuele Scifoni12Pietro Pisciotta13Giorgio Russo14Rosalba Parenti15Department of Medicine and Surgery, Kore University of EnnaInstitute of Bioimaging and Complex Biological Systems, National Research CouncilInstitute of Bioimaging and Complex Biological Systems, National Research CouncilInstitute of Bioimaging and Complex Biological Systems, National Research CouncilInstitute of Bioimaging and Complex Biological Systems, National Research CouncilDepartment of Biomedical and Biotechnological Sciences, University of CataniaNational Institute for Nuclear Physics, Laboratori Nazionali del SudNational Institute for Nuclear Physics, Laboratori Nazionali del SudDepartment of Biomedical and Biotechnological Sciences, University of CataniaDepartment of Biomedical and Biotechnological Sciences, University of CataniaDepartment of Biomedical and Biotechnological Sciences, University of CataniaDepartment of Biomedical and Biotechnological Sciences, University of CataniaNational Institute for Nuclear Physics, Trento Institute for Fundamental Physics and ApplicationsDepartment of Radiation Oncology, University Medical Centre Groningen (UMCG)Institute of Bioimaging and Complex Biological Systems, National Research CouncilDepartment of Biomedical and Biotechnological Sciences, University of CataniaAbstract Recent investigations into radiation-induced side effects have focused on understanding the physiopathological consequences of irradiation on late-responding tissues like the spinal cord, which can lead to chronic progressive myelopathy. Proton therapy, an advanced radiation treatment, aims to minimize damage to healthy tissues through precise dose deposition. However, challenges remain, particularly regarding the variation in dose distribution, characterized by maximum deposition at the end of the proton range, known as the distal fall-off of a spread-out Bragg peak. This variation is critical for nearby organs at risk. In this preliminary study, we evaluated the effects of proton irradiation on the neuronal cell population in mouse spinal cord by comparing two positions of the particle range dose deposition profile. We irradiated the spinal cords at the entrance and the distal edge of the spread-out Bragg peak with a single proton dose. Results showed changes in the expression of synaptophysin, a presynaptic protein crucial for synaptic plasticity. Our findings suggest that examining early radiation-induced physiopathological effects on late-responding tissues can provide valuable insights into neuronal plasticity, informing clinical treatment planning for proton beam positioning.https://doi.org/10.1038/s41598-024-84902-2Synaptic plasticityRBERadiation-induced off-target effectsProton therapyBragg peak |
| spellingShingle | Filippo Torrisi Francesco Paolo Cammarata Valentina Bravatà Marco Calvaruso Cristiana Alberghina Anna Maria Pavone Giada Petringa Giuseppe Antonio Pablo Cirrone Simona Denaro Anna Gervasi Simona D’Aprile Nunzio Vicario Emanuele Scifoni Pietro Pisciotta Giorgio Russo Rosalba Parenti Physiopathological effects of entrance versus distal spread-out Bragg peak on mouse spinal cord neurons Scientific Reports Synaptic plasticity RBE Radiation-induced off-target effects Proton therapy Bragg peak |
| title | Physiopathological effects of entrance versus distal spread-out Bragg peak on mouse spinal cord neurons |
| title_full | Physiopathological effects of entrance versus distal spread-out Bragg peak on mouse spinal cord neurons |
| title_fullStr | Physiopathological effects of entrance versus distal spread-out Bragg peak on mouse spinal cord neurons |
| title_full_unstemmed | Physiopathological effects of entrance versus distal spread-out Bragg peak on mouse spinal cord neurons |
| title_short | Physiopathological effects of entrance versus distal spread-out Bragg peak on mouse spinal cord neurons |
| title_sort | physiopathological effects of entrance versus distal spread out bragg peak on mouse spinal cord neurons |
| topic | Synaptic plasticity RBE Radiation-induced off-target effects Proton therapy Bragg peak |
| url | https://doi.org/10.1038/s41598-024-84902-2 |
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