Hybridization State Detection of DNA-Functionalized Gold Nanoparticles Using Hyperspectral Imaging
Hyperspectral imaging has the unique ability of capturing spectral data for multiple wavelengths at each pixel in an image. This gives the ability to distinguish, with certainty, different nanomaterials and/or distinguish nanomaterials from biological materials. In this study, 4 nm and 13 nm gold na...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Optics |
| Online Access: | http://dx.doi.org/10.1155/2017/8427459 |
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| author | Richard C. Murdock Omar A. Khan Thomas J. Lamkin Saber M. Hussain Nancy Kelley-Loughnane |
| author_facet | Richard C. Murdock Omar A. Khan Thomas J. Lamkin Saber M. Hussain Nancy Kelley-Loughnane |
| author_sort | Richard C. Murdock |
| collection | DOAJ |
| description | Hyperspectral imaging has the unique ability of capturing spectral data for multiple wavelengths at each pixel in an image. This gives the ability to distinguish, with certainty, different nanomaterials and/or distinguish nanomaterials from biological materials. In this study, 4 nm and 13 nm gold nanoparticles (Au NPs) were synthesized, functionalized with complimentary oligonucleotides, and hybridized to form large networks of NPs. Scattering spectra were collected from each sample (unfunctionalized, functionalized, and hybridized) and evaluated. The spectra showed unique peaks for each size of Au NP sample and also exhibited narrowing and intensifying of the spectra as the NPs were functionalized and then subsequently hybridized. These spectra are different from normal aggregation effects where the LSPR and reflected spectrum broaden and are red-shifted. Rather, this appears to be dependent on the ability to control the interparticle distance through oligonucleotide length, which is also investigated through the incorporation of a poly-A spacer. Also, hybridized Au NPs were exposed to cells with no adverse effects and retained their unique spectral signatures. With the ability to distinguish between hybridization states at nearly individual NP levels, this could provide a new method of tracking the intracellular actions of nanomaterials as well as extracellular biosensing applications. |
| format | Article |
| id | doaj-art-06a59fd3ab5f4e8b8b849da797adc5dc |
| institution | Kabale University |
| issn | 1687-9384 1687-9392 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Optics |
| spelling | doaj-art-06a59fd3ab5f4e8b8b849da797adc5dc2025-02-03T06:13:06ZengWileyInternational Journal of Optics1687-93841687-93922017-01-01201710.1155/2017/84274598427459Hybridization State Detection of DNA-Functionalized Gold Nanoparticles Using Hyperspectral ImagingRichard C. Murdock0Omar A. Khan1Thomas J. Lamkin2Saber M. Hussain3Nancy Kelley-Loughnane4Airman Systems Directorate, 711th Human Performance Wing, Wright-Patterson AFB, Human Signatures Branch (711 HPW/RHXB), Dayton, OH, USAAirman Systems Directorate, 711th Human Performance Wing, Wright-Patterson AFB, Molecular Bioeffects Branch (711 HPW/RHDJ), Dayton, OH, USAAirman Systems Directorate, 711th Human Performance Wing, Wright-Patterson AFB, Molecular Bioeffects Branch (711 HPW/RHDJ), Dayton, OH, USAAirman Systems Directorate, 711th Human Performance Wing, Wright-Patterson AFB, Molecular Bioeffects Branch (711 HPW/RHDJ), Dayton, OH, USAAirman Systems Directorate, 711th Human Performance Wing, Wright-Patterson AFB, Human Signatures Branch (711 HPW/RHXB), Dayton, OH, USAHyperspectral imaging has the unique ability of capturing spectral data for multiple wavelengths at each pixel in an image. This gives the ability to distinguish, with certainty, different nanomaterials and/or distinguish nanomaterials from biological materials. In this study, 4 nm and 13 nm gold nanoparticles (Au NPs) were synthesized, functionalized with complimentary oligonucleotides, and hybridized to form large networks of NPs. Scattering spectra were collected from each sample (unfunctionalized, functionalized, and hybridized) and evaluated. The spectra showed unique peaks for each size of Au NP sample and also exhibited narrowing and intensifying of the spectra as the NPs were functionalized and then subsequently hybridized. These spectra are different from normal aggregation effects where the LSPR and reflected spectrum broaden and are red-shifted. Rather, this appears to be dependent on the ability to control the interparticle distance through oligonucleotide length, which is also investigated through the incorporation of a poly-A spacer. Also, hybridized Au NPs were exposed to cells with no adverse effects and retained their unique spectral signatures. With the ability to distinguish between hybridization states at nearly individual NP levels, this could provide a new method of tracking the intracellular actions of nanomaterials as well as extracellular biosensing applications.http://dx.doi.org/10.1155/2017/8427459 |
| spellingShingle | Richard C. Murdock Omar A. Khan Thomas J. Lamkin Saber M. Hussain Nancy Kelley-Loughnane Hybridization State Detection of DNA-Functionalized Gold Nanoparticles Using Hyperspectral Imaging International Journal of Optics |
| title | Hybridization State Detection of DNA-Functionalized Gold Nanoparticles Using Hyperspectral Imaging |
| title_full | Hybridization State Detection of DNA-Functionalized Gold Nanoparticles Using Hyperspectral Imaging |
| title_fullStr | Hybridization State Detection of DNA-Functionalized Gold Nanoparticles Using Hyperspectral Imaging |
| title_full_unstemmed | Hybridization State Detection of DNA-Functionalized Gold Nanoparticles Using Hyperspectral Imaging |
| title_short | Hybridization State Detection of DNA-Functionalized Gold Nanoparticles Using Hyperspectral Imaging |
| title_sort | hybridization state detection of dna functionalized gold nanoparticles using hyperspectral imaging |
| url | http://dx.doi.org/10.1155/2017/8427459 |
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