Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract of Tetradenia riparia against Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda
Dermatophyte infections are a global health problem but neglected in Uganda. This work aimed at determining prevalence of dermatophytosis and antifungal activity of ethanolic crude leaf extract of Tetradenia riparia against dermatophytes isolated from patients attending Kampala International Univers...
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| Format: | Article |
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Wiley
2019-01-01
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| Series: | Dermatology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2019/9328621 |
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| author | Taufik Kakande Yonah Batunge Emmanuel Eilu Ambrose Shabohurira Justus Abimana Saheed Adekunle Akinola Reagan Muhwezi Abubakar Sunusi Adam Sarah Kemuma Onkoba Adamu Almustapha Aliero Collins Atuheire Charles Drago Kato Ibrahim Ntulume |
| author_facet | Taufik Kakande Yonah Batunge Emmanuel Eilu Ambrose Shabohurira Justus Abimana Saheed Adekunle Akinola Reagan Muhwezi Abubakar Sunusi Adam Sarah Kemuma Onkoba Adamu Almustapha Aliero Collins Atuheire Charles Drago Kato Ibrahim Ntulume |
| author_sort | Taufik Kakande |
| collection | DOAJ |
| description | Dermatophyte infections are a global health problem but neglected in Uganda. This work aimed at determining prevalence of dermatophytosis and antifungal activity of ethanolic crude leaf extract of Tetradenia riparia against dermatophytes isolated from patients attending Kampala International University Teaching Hospital (KIU-TH), Uganda. A total of 100 samples of skin and nail scrapings were collected and processed using standard microscopy (KOH) and cultural methods. T. riparia leaves were collected and processed with 95% ethanol using standard extraction method. The crude leaves ethanolic extract was tested against three dermatophytes: Trichophyton tonsurans, T. mentagrophyte, and Microsporum audouinii using modified agar well diffusion method. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the ethanolic leaves crude extract were also determined using broth tube dilution and culture, respectively. Out of 100 samples collected, 49 (49%, 95%CI: 0.3930-0.5876) were found positive for microscopy. The prevalence of dermatophytosis was significantly (p=0.001) associated with age groups of participants with higher infection among those aged 11-20 and 21-30 years with 75.0% each. Out of the 49 that were positive by microscopy, 28 (57.15%, 95% CI: 0.1987-0.3739) were positive by culture. Thirty-one (31) fungal isolates were obtained which included both dermatophyte and non-dermatophyte fungi. T. verrucosum had highest distribution 6 (19.35%) among dermatophytes species while Aspergillus spp. were found to have highest distribution 7 (22.58%) among non-dermatophyte species. The result of the antidermatophytic test showed that T. riparia ethanolic crude leaves extract had activity against tested dermatophytes at 1 g/ml. MIC and MFC of the crude extract of T. riparia against tested dermatophytes ranged from 62.5 to 250 mg/ml and 125 to 500 mg/ml, respectively. The findings of this study reported the presence of dermatophytes causing dermatophytosis among patients attending KIU-TH. The results of the current study showed that T. riparia leaves ethanolic crude extract has antidermatophytic activity against tested dermatophytes. |
| format | Article |
| id | doaj-art-06a0ca315cdb4bf6980f160745bf1dec |
| institution | Kabale University |
| issn | 1687-6105 1687-6113 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
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| series | Dermatology Research and Practice |
| spelling | doaj-art-06a0ca315cdb4bf6980f160745bf1dec2025-02-03T05:46:30ZengWileyDermatology Research and Practice1687-61051687-61132019-01-01201910.1155/2019/93286219328621Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract of Tetradenia riparia against Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, UgandaTaufik Kakande0Yonah Batunge1Emmanuel Eilu2Ambrose Shabohurira3Justus Abimana4Saheed Adekunle Akinola5Reagan Muhwezi6Abubakar Sunusi Adam7Sarah Kemuma Onkoba8Adamu Almustapha Aliero9Collins Atuheire10Charles Drago Kato11Ibrahim Ntulume12School of Pharmacy, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Medical Laboratory Science, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Public Health, Kampala International University Teaching Hospital, P.O. Box 71, Bushenyi, UgandaDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDepartment of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, P.O. Box 71, Bushenyi, UgandaDermatophyte infections are a global health problem but neglected in Uganda. This work aimed at determining prevalence of dermatophytosis and antifungal activity of ethanolic crude leaf extract of Tetradenia riparia against dermatophytes isolated from patients attending Kampala International University Teaching Hospital (KIU-TH), Uganda. A total of 100 samples of skin and nail scrapings were collected and processed using standard microscopy (KOH) and cultural methods. T. riparia leaves were collected and processed with 95% ethanol using standard extraction method. The crude leaves ethanolic extract was tested against three dermatophytes: Trichophyton tonsurans, T. mentagrophyte, and Microsporum audouinii using modified agar well diffusion method. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the ethanolic leaves crude extract were also determined using broth tube dilution and culture, respectively. Out of 100 samples collected, 49 (49%, 95%CI: 0.3930-0.5876) were found positive for microscopy. The prevalence of dermatophytosis was significantly (p=0.001) associated with age groups of participants with higher infection among those aged 11-20 and 21-30 years with 75.0% each. Out of the 49 that were positive by microscopy, 28 (57.15%, 95% CI: 0.1987-0.3739) were positive by culture. Thirty-one (31) fungal isolates were obtained which included both dermatophyte and non-dermatophyte fungi. T. verrucosum had highest distribution 6 (19.35%) among dermatophytes species while Aspergillus spp. were found to have highest distribution 7 (22.58%) among non-dermatophyte species. The result of the antidermatophytic test showed that T. riparia ethanolic crude leaves extract had activity against tested dermatophytes at 1 g/ml. MIC and MFC of the crude extract of T. riparia against tested dermatophytes ranged from 62.5 to 250 mg/ml and 125 to 500 mg/ml, respectively. The findings of this study reported the presence of dermatophytes causing dermatophytosis among patients attending KIU-TH. The results of the current study showed that T. riparia leaves ethanolic crude extract has antidermatophytic activity against tested dermatophytes.http://dx.doi.org/10.1155/2019/9328621 |
| spellingShingle | Taufik Kakande Yonah Batunge Emmanuel Eilu Ambrose Shabohurira Justus Abimana Saheed Adekunle Akinola Reagan Muhwezi Abubakar Sunusi Adam Sarah Kemuma Onkoba Adamu Almustapha Aliero Collins Atuheire Charles Drago Kato Ibrahim Ntulume Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract of Tetradenia riparia against Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda Dermatology Research and Practice |
| title | Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract of Tetradenia riparia against Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda |
| title_full | Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract of Tetradenia riparia against Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda |
| title_fullStr | Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract of Tetradenia riparia against Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda |
| title_full_unstemmed | Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract of Tetradenia riparia against Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda |
| title_short | Prevalence of Dermatophytosis and Antifungal Activity of Ethanolic Crude Leaf Extract of Tetradenia riparia against Dermatophytes Isolated from Patients Attending Kampala International University Teaching Hospital, Uganda |
| title_sort | prevalence of dermatophytosis and antifungal activity of ethanolic crude leaf extract of tetradenia riparia against dermatophytes isolated from patients attending kampala international university teaching hospital uganda |
| url | http://dx.doi.org/10.1155/2019/9328621 |
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