Acupuncture Injection Combined with Electrokinetic Injection for Polydimethylsiloxane Microfluidic Devices
We recently reported acupuncture sample injection that leads to reproducible injection of nL-scale sample segments into a polydimethylsiloxane (PDMS) microchannel for microchip capillary electrophoresis. The advantages of the acupuncture injection in microchip capillary electrophoresis include capab...
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Format: | Article |
Language: | English |
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Wiley
2017-01-01
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Series: | Journal of Analytical Methods in Chemistry |
Online Access: | http://dx.doi.org/10.1155/2017/7495348 |
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author | Ji Won Ha |
author_facet | Ji Won Ha |
author_sort | Ji Won Ha |
collection | DOAJ |
description | We recently reported acupuncture sample injection that leads to reproducible injection of nL-scale sample segments into a polydimethylsiloxane (PDMS) microchannel for microchip capillary electrophoresis. The advantages of the acupuncture injection in microchip capillary electrophoresis include capability of minimizing sample loss and voltage control hardware and capability of introducing sample plugs into any desired position of a microchannel. However, the challenge in the previous study was to achieve reproducible, pL-scale sample injections into PDMS microchannels. In the present study, we introduce an acupuncture injection technique combined with electrokinetic injection (AICEI) technique to inject pL-scale sample segments for microchip capillary electrophoresis. We carried out the capillary zone electrophoresis (CZE) separation of FITC and fluorescein, and the mixture of 10 μM FITC and 10 μM fluorescein was separated completely by using the AICEI method. |
format | Article |
id | doaj-art-069407eff86544709bf309e98431c940 |
institution | Kabale University |
issn | 2090-8865 2090-8873 |
language | English |
publishDate | 2017-01-01 |
publisher | Wiley |
record_format | Article |
series | Journal of Analytical Methods in Chemistry |
spelling | doaj-art-069407eff86544709bf309e98431c9402025-02-03T01:25:54ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732017-01-01201710.1155/2017/74953487495348Acupuncture Injection Combined with Electrokinetic Injection for Polydimethylsiloxane Microfluidic DevicesJi Won Ha0Department of Chemistry, University of Ulsan, 93 Daehak-Ro, Nam-Gu, Ulsan 44610, Republic of KoreaWe recently reported acupuncture sample injection that leads to reproducible injection of nL-scale sample segments into a polydimethylsiloxane (PDMS) microchannel for microchip capillary electrophoresis. The advantages of the acupuncture injection in microchip capillary electrophoresis include capability of minimizing sample loss and voltage control hardware and capability of introducing sample plugs into any desired position of a microchannel. However, the challenge in the previous study was to achieve reproducible, pL-scale sample injections into PDMS microchannels. In the present study, we introduce an acupuncture injection technique combined with electrokinetic injection (AICEI) technique to inject pL-scale sample segments for microchip capillary electrophoresis. We carried out the capillary zone electrophoresis (CZE) separation of FITC and fluorescein, and the mixture of 10 μM FITC and 10 μM fluorescein was separated completely by using the AICEI method.http://dx.doi.org/10.1155/2017/7495348 |
spellingShingle | Ji Won Ha Acupuncture Injection Combined with Electrokinetic Injection for Polydimethylsiloxane Microfluidic Devices Journal of Analytical Methods in Chemistry |
title | Acupuncture Injection Combined with Electrokinetic Injection for Polydimethylsiloxane Microfluidic Devices |
title_full | Acupuncture Injection Combined with Electrokinetic Injection for Polydimethylsiloxane Microfluidic Devices |
title_fullStr | Acupuncture Injection Combined with Electrokinetic Injection for Polydimethylsiloxane Microfluidic Devices |
title_full_unstemmed | Acupuncture Injection Combined with Electrokinetic Injection for Polydimethylsiloxane Microfluidic Devices |
title_short | Acupuncture Injection Combined with Electrokinetic Injection for Polydimethylsiloxane Microfluidic Devices |
title_sort | acupuncture injection combined with electrokinetic injection for polydimethylsiloxane microfluidic devices |
url | http://dx.doi.org/10.1155/2017/7495348 |
work_keys_str_mv | AT jiwonha acupunctureinjectioncombinedwithelectrokineticinjectionforpolydimethylsiloxanemicrofluidicdevices |