Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence
Introduction. Indirect immunofluorescence (IIF) is the gold standard method for the detection of antinuclear antibodies (ANA) which are essential markers for the diagnosis of systemic autoimmune rheumatic diseases. For the discrimination of positive and negative samples, we propose here an original...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/182172 |
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| author | Daniel Bertin Noémie Jourde-Chiche Pierre Bongrand Nathalie Bardin |
| author_facet | Daniel Bertin Noémie Jourde-Chiche Pierre Bongrand Nathalie Bardin |
| author_sort | Daniel Bertin |
| collection | DOAJ |
| description | Introduction. Indirect immunofluorescence (IIF) is the gold standard method for the detection of antinuclear antibodies (ANA) which are essential markers for the diagnosis of systemic autoimmune rheumatic diseases. For the discrimination of positive and negative samples, we propose here an original approach named Immunofluorescence for Computed Antinuclear antibody Rational Evaluation (ICARE) based on the calculation of a fluorescence index (FI). Methods. We made comparison between FI and visual evaluations on 237 consecutive samples and on a cohort of 25 patients with SLE. Results. We obtained very good technical performance of FI (95% sensitivity, 98% specificity, and a kappa of 0.92), even in a subgroup of weakly positive samples. A significant correlation between quantification of FI and IIF ANA titers was found (Spearman's ρ=0.80, P<0.0001). Clinical performance of ICARE was validated on a cohort of patients with SLE corroborating the fact that FI could represent an attractive alternative for the evaluation of antibody titer. Conclusion. Our results represent a major step for automated quantification of IIF ANA, opening attractive perspectives such as rapid sample screening and laboratory standardization. |
| format | Article |
| id | doaj-art-068b7abc2ddc4eeeab0d12d029361298 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-068b7abc2ddc4eeeab0d12d0293612982025-02-03T01:22:38ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/182172182172Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect ImmunofluorescenceDaniel Bertin0Noémie Jourde-Chiche1Pierre Bongrand2Nathalie Bardin3Aix-Marseille Université, Laboratoire d’Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille, 147 boulevard Baille, 13005 Marseille, FranceAix-Marseille Université, Centre de Néphrologie et Transplantation Rénale, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille, 13005 Marseille, FranceAix-Marseille Université, Laboratoire d’Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille, 147 boulevard Baille, 13005 Marseille, FranceAix-Marseille Université, Laboratoire d’Immunologie, Pôle de Biologie, Hôpital de la Conception, Assistance Publique-Hôpitaux de Marseille, 147 boulevard Baille, 13005 Marseille, FranceIntroduction. Indirect immunofluorescence (IIF) is the gold standard method for the detection of antinuclear antibodies (ANA) which are essential markers for the diagnosis of systemic autoimmune rheumatic diseases. For the discrimination of positive and negative samples, we propose here an original approach named Immunofluorescence for Computed Antinuclear antibody Rational Evaluation (ICARE) based on the calculation of a fluorescence index (FI). Methods. We made comparison between FI and visual evaluations on 237 consecutive samples and on a cohort of 25 patients with SLE. Results. We obtained very good technical performance of FI (95% sensitivity, 98% specificity, and a kappa of 0.92), even in a subgroup of weakly positive samples. A significant correlation between quantification of FI and IIF ANA titers was found (Spearman's ρ=0.80, P<0.0001). Clinical performance of ICARE was validated on a cohort of patients with SLE corroborating the fact that FI could represent an attractive alternative for the evaluation of antibody titer. Conclusion. Our results represent a major step for automated quantification of IIF ANA, opening attractive perspectives such as rapid sample screening and laboratory standardization.http://dx.doi.org/10.1155/2013/182172 |
| spellingShingle | Daniel Bertin Noémie Jourde-Chiche Pierre Bongrand Nathalie Bardin Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence Clinical and Developmental Immunology |
| title | Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence |
| title_full | Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence |
| title_fullStr | Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence |
| title_full_unstemmed | Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence |
| title_short | Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence |
| title_sort | original approach for automated quantification of antinuclear autoantibodies by indirect immunofluorescence |
| url | http://dx.doi.org/10.1155/2013/182172 |
| work_keys_str_mv | AT danielbertin originalapproachforautomatedquantificationofantinuclearautoantibodiesbyindirectimmunofluorescence AT noemiejourdechiche originalapproachforautomatedquantificationofantinuclearautoantibodiesbyindirectimmunofluorescence AT pierrebongrand originalapproachforautomatedquantificationofantinuclearautoantibodiesbyindirectimmunofluorescence AT nathaliebardin originalapproachforautomatedquantificationofantinuclearautoantibodiesbyindirectimmunofluorescence |