Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal Fibrosis
The Th2 immune response in chronic schistosomiasis is associated with the development of periportal fibrosis. However, little is known about the phenotype and activation status of T cells in the process. Objective. To evaluate the profile of T cells in schistosomiasis patients with periportal fibros...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/710647 |
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| author | Luciana Santos Cardoso Andréia de Souza Rocha Barreto Jamille Souza Fernandes Ricardo Riccio Oliveira Robson da Paixão de Souza Edgar M. Carvalho Maria Ilma Araujo |
| author_facet | Luciana Santos Cardoso Andréia de Souza Rocha Barreto Jamille Souza Fernandes Ricardo Riccio Oliveira Robson da Paixão de Souza Edgar M. Carvalho Maria Ilma Araujo |
| author_sort | Luciana Santos Cardoso |
| collection | DOAJ |
| description | The Th2 immune response in chronic schistosomiasis is associated with the development of periportal fibrosis. However, little is known about the phenotype and activation status of T cells in the process. Objective. To evaluate the profile of T cells in schistosomiasis patients with periportal fibrosis. Methods. It was a cross-sectional study, conducted in the village of Agua Preta, Bahia, Brazil, which included 37 subjects with periportal fibrosis determined by ultrasound. Peripheral blood mononuclear cells were obtained by the Ficcol-hypaque gradient and the frequency of T cells expressing the surface markers CD28, CD69, CD25, and CTLA-4 was determined by flow cytometry. Results. The frequency of CD4+CD28+ T lymphocytes was higher in individuals with moderate to severe fibrosis compared to patients with incipient fibrosis. We did not observe any significant difference in the frequency of CD4+ T cells expressing CD69 among groups of individuals. There was also no significant difference in the frequency of CD8+ T cells expressing CD28 or CD69 among the studied groups. Individuals with moderate to severe fibrosis presented a lower frequency of CD8+ T cells, CD4+CD25high T cells, and CD4+CTLA-4+ T cells when compared to patients without fibrosis or incipient fibrosis. The frequency of CD4+CD25low cells did not differ between groups. Conclusion. The high frequency of activated T cells coinciding with a low frequency of putative Treg cells may account for the development of periportal fibrosis in human schistosomiasis. |
| format | Article |
| id | doaj-art-0674e876c3934f15939dbee65b67161f |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-0674e876c3934f15939dbee65b67161f2025-02-03T05:52:20ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/710647710647Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal FibrosisLuciana Santos Cardoso0Andréia de Souza Rocha Barreto1Jamille Souza Fernandes2Ricardo Riccio Oliveira3Robson da Paixão de Souza4Edgar M. Carvalho5Maria Ilma Araujo6Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia (UFBA), 4110-160 Salvador-BA, BrazilServiço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia (UFBA), 4110-160 Salvador-BA, BrazilServiço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia (UFBA), 4110-160 Salvador-BA, BrazilServiço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia (UFBA), 4110-160 Salvador-BA, BrazilServiço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia (UFBA), 4110-160 Salvador-BA, BrazilServiço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia (UFBA), 4110-160 Salvador-BA, BrazilServiço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia (UFBA), 4110-160 Salvador-BA, BrazilThe Th2 immune response in chronic schistosomiasis is associated with the development of periportal fibrosis. However, little is known about the phenotype and activation status of T cells in the process. Objective. To evaluate the profile of T cells in schistosomiasis patients with periportal fibrosis. Methods. It was a cross-sectional study, conducted in the village of Agua Preta, Bahia, Brazil, which included 37 subjects with periportal fibrosis determined by ultrasound. Peripheral blood mononuclear cells were obtained by the Ficcol-hypaque gradient and the frequency of T cells expressing the surface markers CD28, CD69, CD25, and CTLA-4 was determined by flow cytometry. Results. The frequency of CD4+CD28+ T lymphocytes was higher in individuals with moderate to severe fibrosis compared to patients with incipient fibrosis. We did not observe any significant difference in the frequency of CD4+ T cells expressing CD69 among groups of individuals. There was also no significant difference in the frequency of CD8+ T cells expressing CD28 or CD69 among the studied groups. Individuals with moderate to severe fibrosis presented a lower frequency of CD8+ T cells, CD4+CD25high T cells, and CD4+CTLA-4+ T cells when compared to patients without fibrosis or incipient fibrosis. The frequency of CD4+CD25low cells did not differ between groups. Conclusion. The high frequency of activated T cells coinciding with a low frequency of putative Treg cells may account for the development of periportal fibrosis in human schistosomiasis.http://dx.doi.org/10.1155/2013/710647 |
| spellingShingle | Luciana Santos Cardoso Andréia de Souza Rocha Barreto Jamille Souza Fernandes Ricardo Riccio Oliveira Robson da Paixão de Souza Edgar M. Carvalho Maria Ilma Araujo Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal Fibrosis Clinical and Developmental Immunology |
| title | Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal Fibrosis |
| title_full | Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal Fibrosis |
| title_fullStr | Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal Fibrosis |
| title_full_unstemmed | Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal Fibrosis |
| title_short | Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal Fibrosis |
| title_sort | impaired lymphocyte profile in schistosomiasis patients with periportal fibrosis |
| url | http://dx.doi.org/10.1155/2013/710647 |
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