Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against Pseudomonas aeruginosa

ABSTRACT Pyroptosis is an inflammatory immune response of eukaryotic cells to bacterial lipopolysaccharide (LPS) and other pathological stimuli, leading to the activation of the gasdermin D (GSDMD) and secretion of pore-forming domain GSDMDNterm, facilitating the release of cytokines. Additionally,...

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Main Authors: Dorota Kuc-Ciepluch, Karol Ciepluch, Daria Augustyniak, Grzegorz Guła, Barbara Maciejewska, Artur Kowalik, Ewelina Jop, Zuzanna Drulis-Kawa, Michał Arabski
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Language:English
Published: American Society for Microbiology 2025-01-01
Series:mSystems
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Online Access:https://journals.asm.org/doi/10.1128/msystems.01106-24
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author Dorota Kuc-Ciepluch
Karol Ciepluch
Daria Augustyniak
Grzegorz Guła
Barbara Maciejewska
Artur Kowalik
Ewelina Jop
Zuzanna Drulis-Kawa
Michał Arabski
author_facet Dorota Kuc-Ciepluch
Karol Ciepluch
Daria Augustyniak
Grzegorz Guła
Barbara Maciejewska
Artur Kowalik
Ewelina Jop
Zuzanna Drulis-Kawa
Michał Arabski
author_sort Dorota Kuc-Ciepluch
collection DOAJ
description ABSTRACT Pyroptosis is an inflammatory immune response of eukaryotic cells to bacterial lipopolysaccharide (LPS) and other pathological stimuli, leading to the activation of the gasdermin D (GSDMD) and secretion of pore-forming domain GSDMDNterm, facilitating the release of cytokines. Additionally, GSDMDNterm exhibits antibacterial properties through interactions with bacterial outer membranes (OM). We explored alternative antimicrobial strategy to determine whether inducing natural pyroptosis via GSDMD activation by P. aeruginosa LPS could enhance the effectiveness of recombinant phage endopeptidase KP27 (peptidoglycan-degrading enzyme) against P. aeruginosa, enabling penetration through OM and bacterial killing synergistically. Our findings demonstrated that recombinant GSDMD alone exhibited antibacterial effects against wild-type P. aeruginosa with smooth LPS, while recombinant GSDMDNterm efficiently permeabilized both smooth LPS-bearing and O-chain-deficient P. aeruginosa potentially synergizing with endolysin KP27. Transcriptomic analyses revealed the activation of the immune system pathways in response to LPS, mainly in monocytic cells, in contrast to epithelial A549 or HeLa cell lines. LPS-induced pyroptosis in monocytes led to GSDMD cleavage and the release of interleukins, regardless of the nature/origin of the LPS used. However, the pyroptosis stimulation by LPS in the antibacterial assay was not effective enough for bacterial OM permeabilization and enhancement of endolysin activity. We assume that leveraging pyroptosis induction in monocytic cells to augment the bactericidal activity of endolysins may be limited.IMPORTANCERecombinant GSDMDNterm protein was able to efficiently permeabilize P. aeruginosa outer membranes and increase endolysin activity against bacteria, producing either long LPS O-chains or lack them entirely. The obtained results suggest the limited possibility of using the natural process of pyroptosis occurring in monocytic cells to enhance the bactericidal effect of recombinant phage endolysins against Gram-negative bacteria infection.
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institution Kabale University
issn 2379-5077
language English
publishDate 2025-01-01
publisher American Society for Microbiology
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spelling doaj-art-066fda5f197a470c9748ae75adc0096c2025-01-21T14:00:28ZengAmerican Society for MicrobiologymSystems2379-50772025-01-0110110.1128/msystems.01106-24Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against Pseudomonas aeruginosaDorota Kuc-Ciepluch0Karol Ciepluch1Daria Augustyniak2Grzegorz Guła3Barbara Maciejewska4Artur Kowalik5Ewelina Jop6Zuzanna Drulis-Kawa7Michał Arabski8Division of Medical Biology, Jan Kochanowski University in Kielce, Kielce, PolandDivision of Medical Biology, Jan Kochanowski University in Kielce, Kielce, PolandDepartment of Pathogen Biology and Immunology, University of Wroclaw, Wroclaw, PolandDepartment of Pathogen Biology and Immunology, University of Wroclaw, Wroclaw, PolandDepartment of Pathogen Biology and Immunology, University of Wroclaw, Wroclaw, PolandDivision of Medical Biology, Jan Kochanowski University in Kielce, Kielce, PolandDepartment of Pathogen Biology and Immunology, University of Wroclaw, Wroclaw, PolandDepartment of Pathogen Biology and Immunology, University of Wroclaw, Wroclaw, PolandDivision of Medical Biology, Jan Kochanowski University in Kielce, Kielce, PolandABSTRACT Pyroptosis is an inflammatory immune response of eukaryotic cells to bacterial lipopolysaccharide (LPS) and other pathological stimuli, leading to the activation of the gasdermin D (GSDMD) and secretion of pore-forming domain GSDMDNterm, facilitating the release of cytokines. Additionally, GSDMDNterm exhibits antibacterial properties through interactions with bacterial outer membranes (OM). We explored alternative antimicrobial strategy to determine whether inducing natural pyroptosis via GSDMD activation by P. aeruginosa LPS could enhance the effectiveness of recombinant phage endopeptidase KP27 (peptidoglycan-degrading enzyme) against P. aeruginosa, enabling penetration through OM and bacterial killing synergistically. Our findings demonstrated that recombinant GSDMD alone exhibited antibacterial effects against wild-type P. aeruginosa with smooth LPS, while recombinant GSDMDNterm efficiently permeabilized both smooth LPS-bearing and O-chain-deficient P. aeruginosa potentially synergizing with endolysin KP27. Transcriptomic analyses revealed the activation of the immune system pathways in response to LPS, mainly in monocytic cells, in contrast to epithelial A549 or HeLa cell lines. LPS-induced pyroptosis in monocytes led to GSDMD cleavage and the release of interleukins, regardless of the nature/origin of the LPS used. However, the pyroptosis stimulation by LPS in the antibacterial assay was not effective enough for bacterial OM permeabilization and enhancement of endolysin activity. We assume that leveraging pyroptosis induction in monocytic cells to augment the bactericidal activity of endolysins may be limited.IMPORTANCERecombinant GSDMDNterm protein was able to efficiently permeabilize P. aeruginosa outer membranes and increase endolysin activity against bacteria, producing either long LPS O-chains or lack them entirely. The obtained results suggest the limited possibility of using the natural process of pyroptosis occurring in monocytic cells to enhance the bactericidal effect of recombinant phage endolysins against Gram-negative bacteria infection.https://journals.asm.org/doi/10.1128/msystems.01106-24Pseudomonas aeruginosa LPSpyroptosisgasdermin Dendolysinouter membrane permeabilization
spellingShingle Dorota Kuc-Ciepluch
Karol Ciepluch
Daria Augustyniak
Grzegorz Guła
Barbara Maciejewska
Artur Kowalik
Ewelina Jop
Zuzanna Drulis-Kawa
Michał Arabski
Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against Pseudomonas aeruginosa
mSystems
Pseudomonas aeruginosa LPS
pyroptosis
gasdermin D
endolysin
outer membrane permeabilization
title Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against Pseudomonas aeruginosa
title_full Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against Pseudomonas aeruginosa
title_fullStr Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against Pseudomonas aeruginosa
title_full_unstemmed Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against Pseudomonas aeruginosa
title_short Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against Pseudomonas aeruginosa
title_sort exploiting gasdermin mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against pseudomonas aeruginosa
topic Pseudomonas aeruginosa LPS
pyroptosis
gasdermin D
endolysin
outer membrane permeabilization
url https://journals.asm.org/doi/10.1128/msystems.01106-24
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