Protocol for in vivo recording of neural activity in deep structures of mice brain via gradient lenses by calcium imaging
Summary: Calcium (Ca2+) imaging is a viable approach for imaging neuronal activity patterns in local brain circuits in living animals. Here, we present a protocol for gradient lens implantation in deep brain structures followed by in vivo Ca2+ imaging. We describe in detail the steps for surgery pre...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724006993 |
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| Summary: | Summary: Calcium (Ca2+) imaging is a viable approach for imaging neuronal activity patterns in local brain circuits in living animals. Here, we present a protocol for gradient lens implantation in deep brain structures followed by in vivo Ca2+ imaging. We describe in detail the steps for surgery preparation, followed by lens implantation, setup for awake head-fixed imaging, and the recording process.For complete details on the use and execution of this protocol, please refer to Cherepanov et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |