Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro
Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the abilit...
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Faculty of Medicine at Universitas Andalas
2021-06-01
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| Series: | Jurnal Kesehatan Andalas |
| Online Access: | http://jurnal.fk.unand.ac.id/index.php/jka/article/view/1595 |
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| author | Saskia Arientika Wahyuningrum Meiskha Bahar Andri Pramesyanti Pramono |
| author_facet | Saskia Arientika Wahyuningrum Meiskha Bahar Andri Pramesyanti Pramono |
| author_sort | Saskia Arientika Wahyuningrum |
| collection | DOAJ |
| description | Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.
Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa |
| format | Article |
| id | doaj-art-065fb74dc125461d8628c71b2d19b961 |
| institution | Kabale University |
| issn | 2301-7406 2615-1138 |
| language | English |
| publishDate | 2021-06-01 |
| publisher | Faculty of Medicine at Universitas Andalas |
| record_format | Article |
| series | Jurnal Kesehatan Andalas |
| spelling | doaj-art-065fb74dc125461d8628c71b2d19b9612025-02-03T03:39:22ZengFaculty of Medicine at Universitas AndalasJurnal Kesehatan Andalas2301-74062615-11382021-06-01101162210.25077/jka.v10i1.15951174Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In VitroSaskia Arientika Wahyuningrum0Meiskha Bahar1Andri Pramesyanti Pramono2Fakultas Kedokteran, Universitas Pembangunan Nasional Veteran, JakartaDepartemen Mikrobiologi, Fakultas Kedokteran, Universitas Pembangunan Nasional Veteran, JakartaDepartemen Mikrobiologi, Fakultas Kedokteran, Universitas Pembangunan Nasional Veteran, JakartaPneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power. Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosahttp://jurnal.fk.unand.ac.id/index.php/jka/article/view/1595 |
| spellingShingle | Saskia Arientika Wahyuningrum Meiskha Bahar Andri Pramesyanti Pramono Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro Jurnal Kesehatan Andalas |
| title | Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro |
| title_full | Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro |
| title_fullStr | Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro |
| title_full_unstemmed | Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro |
| title_short | Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro |
| title_sort | uji daya hambat isolat actinomycetes sebagai antibakteri terhadap pertumbuhan pseudomonas aeruginosa atcc 27853 secara in vitro |
| url | http://jurnal.fk.unand.ac.id/index.php/jka/article/view/1595 |
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