Effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice
Multidrug-resistant bacteria are a clinical and an epidemiological challenge. However, the effects of multidrug-resistant bacteria and multi-antibiotic combination on gut microbiota are unclear. In this study, the effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1504396/full |
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| author | Jing Ma Zheng Gong Meiling Kang Zhongjing Tian Liping You Chengshi Ding |
| author_facet | Jing Ma Zheng Gong Meiling Kang Zhongjing Tian Liping You Chengshi Ding |
| author_sort | Jing Ma |
| collection | DOAJ |
| description | Multidrug-resistant bacteria are a clinical and an epidemiological challenge. However, the effects of multidrug-resistant bacteria and multi-antibiotic combination on gut microbiota are unclear. In this study, the effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice have been observed by high-throughput sequencing. Resistant Escherichia coli (RP4) and 0.5, 1, and 2 mg/L antibiotics (Amp, Km, and Tet multi-antibiotic combination) could decrease the number of specific operational taxonomic units from 223 in the normal saline control group to 178 in the antibiotic-resistant bacteria group and 34 in the antibiotic group, and antibiotics are the biggest influencing factor. Multidrug-resistant bacteria and multi-antibiotic combination could affect the function of intestinal microbiota, and the effect of multidrug-resistant bacteria was similar to that of multi-antibiotic combination. Small intestine is the main colonization site of antibiotic-resistant bacteria, and Proteobacteria and Bacteroidetes are the major antibiotic-resistance acquired bacteria as determined by transmission electron microscopy and agarose plate screening culture. |
| format | Article |
| id | doaj-art-063c0eb7719148a6b1257417db806c67 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-063c0eb7719148a6b1257417db806c672025-02-05T07:32:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011510.3389/fmicb.2024.15043961504396Effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in miceJing Ma0Zheng Gong1Meiling Kang2Zhongjing Tian3Liping You4Chengshi Ding5College of Life Sciences, Zaozhuang University, Zaozhuang, ChinaCollege of Life Sciences, Zaozhuang University, Zaozhuang, ChinaCollege of Life Sciences, Zaozhuang University, Zaozhuang, ChinaCollege of Life Sciences, Zaozhuang University, Zaozhuang, ChinaDepartment of Clinical Laboratory, Zibo Central Hospital, Zibo, ChinaCollege of Life Sciences, Zaozhuang University, Zaozhuang, ChinaMultidrug-resistant bacteria are a clinical and an epidemiological challenge. However, the effects of multidrug-resistant bacteria and multi-antibiotic combination on gut microbiota are unclear. In this study, the effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice have been observed by high-throughput sequencing. Resistant Escherichia coli (RP4) and 0.5, 1, and 2 mg/L antibiotics (Amp, Km, and Tet multi-antibiotic combination) could decrease the number of specific operational taxonomic units from 223 in the normal saline control group to 178 in the antibiotic-resistant bacteria group and 34 in the antibiotic group, and antibiotics are the biggest influencing factor. Multidrug-resistant bacteria and multi-antibiotic combination could affect the function of intestinal microbiota, and the effect of multidrug-resistant bacteria was similar to that of multi-antibiotic combination. Small intestine is the main colonization site of antibiotic-resistant bacteria, and Proteobacteria and Bacteroidetes are the major antibiotic-resistance acquired bacteria as determined by transmission electron microscopy and agarose plate screening culture.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1504396/fullmultidrug-resistant Escherichia colimulti-antibiotic combinationintestinal microbiotahigh-throughput sequencingantibiotics |
| spellingShingle | Jing Ma Zheng Gong Meiling Kang Zhongjing Tian Liping You Chengshi Ding Effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice Frontiers in Microbiology multidrug-resistant Escherichia coli multi-antibiotic combination intestinal microbiota high-throughput sequencing antibiotics |
| title | Effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice |
| title_full | Effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice |
| title_fullStr | Effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice |
| title_full_unstemmed | Effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice |
| title_short | Effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice |
| title_sort | effects of multidrug resistant bacteria and multi antibiotic combination on intestinal microbiota in mice |
| topic | multidrug-resistant Escherichia coli multi-antibiotic combination intestinal microbiota high-throughput sequencing antibiotics |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1504396/full |
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