Photobiomodulation on Bax and Bcl-2 Proteins and SIRT1/PGC-1α Axis mRNA Expression Levels of Aging Rat Skeletal Muscle
Objective. This study aimed to analyze the effects of low level laser irradiation (LLLI) on Bax and IGF-1 and Bcl-2 protein contents and SIRT1/PGC-1α axis mRNA expression levels to prevent sarcopenia in aged rats. Material and Methods. Twenty female Sprague Dawley rats (18 months old) were randomly...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/2014/384816 |
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| author | Fang-Hui Li Yan-Ying Liu Fei Qin Qing Luo Hai-Ping Yang Quan-Guang Zhang Timon Cheng-Yi Liu |
| author_facet | Fang-Hui Li Yan-Ying Liu Fei Qin Qing Luo Hai-Ping Yang Quan-Guang Zhang Timon Cheng-Yi Liu |
| author_sort | Fang-Hui Li |
| collection | DOAJ |
| description | Objective. This study aimed to analyze the effects of low level laser irradiation (LLLI) on Bax and IGF-1 and Bcl-2 protein contents and SIRT1/PGC-1α axis mRNA expression levels to prevent sarcopenia in aged rats. Material and Methods. Twenty female Sprague Dawley rats (18 months old) were randomly divided into two groups (n=10 per group): control (CON) and LLLI groups. The gallium-aluminum-arsenium (GaAlAs) laser irradiation at 810 nm was used in the single point contact mode (3.75 J/cm2; 0.4 cm2; 125 mW/cm2; 30 s). Bax, Bcl-2, and IGF-1 proteins and SIRT1/PGC-1α axis mRNA expression were assessed 24 h after LLLI on gastrocnemius in aged rat. Results. Gastrocnemius muscle weights, gastrocnemius mass/body mass, Bcl-2/BAX ratio, Bcl-2 protein, IGF-1 protein, and the mRNA contents in SIRT1, PGC-1α, NRF1, TMF, and SOD2 were significantly (P<0.05) increased by LLLI compared to CON group without LLLI. However, levels of BAX protein and caspase 3 mRNA were significantly attenuated by LLLI compared to CON group (P<0.05). Conclusion. LLLI at 810 nm inhibits sarcopenia associated with upregulation of Bcl-2/BAX ratio and IGF-1 and SIRT1/PGC-1α axis mRNA expression in aged rats. This indicates that LLLI has potential to decrease progression of myocyte apoptosis in sarcopenic muscles. |
| format | Article |
| id | doaj-art-0627964257b649a0ab0b178cc81e3328 |
| institution | Kabale University |
| issn | 1110-662X 1687-529X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Photoenergy |
| spelling | doaj-art-0627964257b649a0ab0b178cc81e33282025-02-03T01:02:55ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2014-01-01201410.1155/2014/384816384816Photobiomodulation on Bax and Bcl-2 Proteins and SIRT1/PGC-1α Axis mRNA Expression Levels of Aging Rat Skeletal MuscleFang-Hui Li0Yan-Ying Liu1Fei Qin2Qing Luo3Hai-Ping Yang4Quan-Guang Zhang5Timon Cheng-Yi Liu6Laboratory of Laser Sports Medicine, South China Normal University, University Town, Guangzhou 510006, ChinaLaboratory of Laser Sports Medicine, South China Normal University, University Town, Guangzhou 510006, ChinaLaboratory of Laser Sports Medicine, South China Normal University, University Town, Guangzhou 510006, ChinaTeaching Department of Ideological and Political Theory, Zhaoqing University, Zhaoqing 526061, ChinaSchool of Physical Education and Health, Zhaoqing University, Zhaoqing 526061, ChinaInstitute of Molecular Medicine and Genetics, Medical College of Georgia at Georgia Regents University, Augusta, GA 30912, USALaboratory of Laser Sports Medicine, South China Normal University, University Town, Guangzhou 510006, ChinaObjective. This study aimed to analyze the effects of low level laser irradiation (LLLI) on Bax and IGF-1 and Bcl-2 protein contents and SIRT1/PGC-1α axis mRNA expression levels to prevent sarcopenia in aged rats. Material and Methods. Twenty female Sprague Dawley rats (18 months old) were randomly divided into two groups (n=10 per group): control (CON) and LLLI groups. The gallium-aluminum-arsenium (GaAlAs) laser irradiation at 810 nm was used in the single point contact mode (3.75 J/cm2; 0.4 cm2; 125 mW/cm2; 30 s). Bax, Bcl-2, and IGF-1 proteins and SIRT1/PGC-1α axis mRNA expression were assessed 24 h after LLLI on gastrocnemius in aged rat. Results. Gastrocnemius muscle weights, gastrocnemius mass/body mass, Bcl-2/BAX ratio, Bcl-2 protein, IGF-1 protein, and the mRNA contents in SIRT1, PGC-1α, NRF1, TMF, and SOD2 were significantly (P<0.05) increased by LLLI compared to CON group without LLLI. However, levels of BAX protein and caspase 3 mRNA were significantly attenuated by LLLI compared to CON group (P<0.05). Conclusion. LLLI at 810 nm inhibits sarcopenia associated with upregulation of Bcl-2/BAX ratio and IGF-1 and SIRT1/PGC-1α axis mRNA expression in aged rats. This indicates that LLLI has potential to decrease progression of myocyte apoptosis in sarcopenic muscles.http://dx.doi.org/10.1155/2014/384816 |
| spellingShingle | Fang-Hui Li Yan-Ying Liu Fei Qin Qing Luo Hai-Ping Yang Quan-Guang Zhang Timon Cheng-Yi Liu Photobiomodulation on Bax and Bcl-2 Proteins and SIRT1/PGC-1α Axis mRNA Expression Levels of Aging Rat Skeletal Muscle International Journal of Photoenergy |
| title | Photobiomodulation on Bax and Bcl-2 Proteins and SIRT1/PGC-1α Axis mRNA Expression Levels of Aging Rat Skeletal Muscle |
| title_full | Photobiomodulation on Bax and Bcl-2 Proteins and SIRT1/PGC-1α Axis mRNA Expression Levels of Aging Rat Skeletal Muscle |
| title_fullStr | Photobiomodulation on Bax and Bcl-2 Proteins and SIRT1/PGC-1α Axis mRNA Expression Levels of Aging Rat Skeletal Muscle |
| title_full_unstemmed | Photobiomodulation on Bax and Bcl-2 Proteins and SIRT1/PGC-1α Axis mRNA Expression Levels of Aging Rat Skeletal Muscle |
| title_short | Photobiomodulation on Bax and Bcl-2 Proteins and SIRT1/PGC-1α Axis mRNA Expression Levels of Aging Rat Skeletal Muscle |
| title_sort | photobiomodulation on bax and bcl 2 proteins and sirt1 pgc 1α axis mrna expression levels of aging rat skeletal muscle |
| url | http://dx.doi.org/10.1155/2014/384816 |
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