Maximizing the neuroprotection from Citrus aurantium leaves: Optimization of a blended extract from a sequential extraction process with compressed fluids and NADES
Citrus aurantium, also known as bitter orange, and the by-products obtained during its processing are a rich source of bioactive compounds. In particular, leaves from the tree pruning and from fruits harvesting are considered an agrifood-related waste with high valorization potential. Considering th...
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Elsevier
2025-02-01
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| Series: | Advances in Sample Preparation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772582025000038 |
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| author | Victor M. Amador-Luna Miguel Herrero Marta Jiménez de la Parra Ángel Gómez Arribas Elena Ibáñez Lidia Montero |
| author_facet | Victor M. Amador-Luna Miguel Herrero Marta Jiménez de la Parra Ángel Gómez Arribas Elena Ibáñez Lidia Montero |
| author_sort | Victor M. Amador-Luna |
| collection | DOAJ |
| description | Citrus aurantium, also known as bitter orange, and the by-products obtained during its processing are a rich source of bioactive compounds. In particular, leaves from the tree pruning and from fruits harvesting are considered an agrifood-related waste with high valorization potential. Considering the wide variety of bioactive compounds present in the leaves (carotenoids, terpenes and phenolic compounds), this study is focused on the valorization of C. aurantium pruning waste through a sequential three-step extraction process using green extraction techniques, i.e. supercritical fluid extraction (SFE), followed by pressurized liquid extraction (PLE) using natural deep eutectic solvents (NADES), and an antisolvent extraction to recover the remaining bioactive compounds adsorbed to the NADES and extraction residues. In this sense, this is the first time that PLE using NADES followed by an antisolvent step to re-extract the compounds from the PLE residue is presented. Moreover, different protocols to efficiently remove the NADES from the PLE extract were also studied for the first time. Due to the different nature and bioactivity of the obtained extracts, the mixture of the three fractions was optimized to enhance its overall preventive potential against neurodegenerative diseases. Finally, its scalability to a semi-pilot scale was evaluated, giving rise to increased bioactive values of the blended extract. |
| format | Article |
| id | doaj-art-0613ea0e8eae4c47bfc4f452725c2624 |
| institution | Kabale University |
| issn | 2772-5820 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Sample Preparation |
| spelling | doaj-art-0613ea0e8eae4c47bfc4f452725c26242025-01-19T06:26:58ZengElsevierAdvances in Sample Preparation2772-58202025-02-0113100149Maximizing the neuroprotection from Citrus aurantium leaves: Optimization of a blended extract from a sequential extraction process with compressed fluids and NADESVictor M. Amador-Luna0Miguel Herrero1Marta Jiménez de la Parra2Ángel Gómez Arribas3Elena Ibáñez4Lidia Montero5Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC Nicolas Cabrera 9, 28049 Madrid, SpainLaboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC Nicolas Cabrera 9, 28049 Madrid, SpainLaboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC Nicolas Cabrera 9, 28049 Madrid, SpainLaboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC Nicolas Cabrera 9, 28049 Madrid, SpainLaboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC Nicolas Cabrera 9, 28049 Madrid, SpainCorresponding author.; Laboratory of Foodomics, Institute of Food Science Research, CIAL, CSIC Nicolas Cabrera 9, 28049 Madrid, SpainCitrus aurantium, also known as bitter orange, and the by-products obtained during its processing are a rich source of bioactive compounds. In particular, leaves from the tree pruning and from fruits harvesting are considered an agrifood-related waste with high valorization potential. Considering the wide variety of bioactive compounds present in the leaves (carotenoids, terpenes and phenolic compounds), this study is focused on the valorization of C. aurantium pruning waste through a sequential three-step extraction process using green extraction techniques, i.e. supercritical fluid extraction (SFE), followed by pressurized liquid extraction (PLE) using natural deep eutectic solvents (NADES), and an antisolvent extraction to recover the remaining bioactive compounds adsorbed to the NADES and extraction residues. In this sense, this is the first time that PLE using NADES followed by an antisolvent step to re-extract the compounds from the PLE residue is presented. Moreover, different protocols to efficiently remove the NADES from the PLE extract were also studied for the first time. Due to the different nature and bioactivity of the obtained extracts, the mixture of the three fractions was optimized to enhance its overall preventive potential against neurodegenerative diseases. Finally, its scalability to a semi-pilot scale was evaluated, giving rise to increased bioactive values of the blended extract.http://www.sciencedirect.com/science/article/pii/S2772582025000038Blended extractCitrus aurantium leavesNADES removalPLEScale-upSupercritical fluid extraction |
| spellingShingle | Victor M. Amador-Luna Miguel Herrero Marta Jiménez de la Parra Ángel Gómez Arribas Elena Ibáñez Lidia Montero Maximizing the neuroprotection from Citrus aurantium leaves: Optimization of a blended extract from a sequential extraction process with compressed fluids and NADES Advances in Sample Preparation Blended extract Citrus aurantium leaves NADES removal PLE Scale-up Supercritical fluid extraction |
| title | Maximizing the neuroprotection from Citrus aurantium leaves: Optimization of a blended extract from a sequential extraction process with compressed fluids and NADES |
| title_full | Maximizing the neuroprotection from Citrus aurantium leaves: Optimization of a blended extract from a sequential extraction process with compressed fluids and NADES |
| title_fullStr | Maximizing the neuroprotection from Citrus aurantium leaves: Optimization of a blended extract from a sequential extraction process with compressed fluids and NADES |
| title_full_unstemmed | Maximizing the neuroprotection from Citrus aurantium leaves: Optimization of a blended extract from a sequential extraction process with compressed fluids and NADES |
| title_short | Maximizing the neuroprotection from Citrus aurantium leaves: Optimization of a blended extract from a sequential extraction process with compressed fluids and NADES |
| title_sort | maximizing the neuroprotection from citrus aurantium leaves optimization of a blended extract from a sequential extraction process with compressed fluids and nades |
| topic | Blended extract Citrus aurantium leaves NADES removal PLE Scale-up Supercritical fluid extraction |
| url | http://www.sciencedirect.com/science/article/pii/S2772582025000038 |
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