Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma
Objective. To observe the effects of empirical prescriptions of traditional Chinese medicine (TCM) on inflammatory mediators in pediatric asthma and to explore the underlying molecular mechanism in the treatment of asthma. Methods. A total of 182 children with asthma were randomly placed into either...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/5143703 |
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| author | Hui Du Yonghong Wang Yumin Shi Jian Yu Wen Sun Yiqun Zhang |
| author_facet | Hui Du Yonghong Wang Yumin Shi Jian Yu Wen Sun Yiqun Zhang |
| author_sort | Hui Du |
| collection | DOAJ |
| description | Objective. To observe the effects of empirical prescriptions of traditional Chinese medicine (TCM) on inflammatory mediators in pediatric asthma and to explore the underlying molecular mechanism in the treatment of asthma. Methods. A total of 182 children with asthma were randomly placed into either the TCM group (n=97) or the salbutamol and montelukast (SM) group (n=85). Patients in the TCM group were treated with a series of empirical prescriptions of TCM, while those in the SM group received salbutamol and montelukast. Both groups received their respective treatment for 12 weeks. There were 35 patients in TCM group and 34 patients in SM group providing venous blood. Real-time PCR was used to determine the mRNA expression levels of interleukin- (IL-) 10, IL-17, matrix metalloproteinase-9 (MMP-9), and transforming growth factor β1 (TGF-β1) in peripheral blood mononuclear cells before and after treatment. Enzyme-linked immunosorbent assay was used to measure the levels of IL-10, IL-17, MMP-9, and TGF-β1 in peripheral blood before and after treatment. Results. The mRNA expression of TGF-β1 in the SM group was downregulated (P=0.00) after treatment. No significant differences were found between the TCM group and the SM group after treatment (P>0.05). In the TCM group, the levels of IL-10, IL-17, and MMP-9 significantly decreased after treatment (P=0.01, 0.04, and 0.03, resp.). In the SM group, IL-17, MMP-9, and TGF-β1 levels significantly decreased after treatment (P=0.00, 0.03, and 0.00, resp.). There was no significant difference between the two groups regarding the levels of IL-10, IL-17, TGF-β1, and MMP-9 (P>0.05). The difference of the level of IL-17 was negatively correlated with the change of C-ACT score in TCM group and SM group. Conclusion. TCM has a regulatory effect on the balance of some inflammatory mediators in pediatric asthma. |
| format | Article |
| id | doaj-art-0606e86d60c540f79b914f36d2bbcf3b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-0606e86d60c540f79b914f36d2bbcf3b2025-02-03T05:51:55ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/51437035143703Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric AsthmaHui Du0Yonghong Wang1Yumin Shi2Jian Yu3Wen Sun4Yiqun Zhang5Department of Traditional Chinese Medicine, Children’s Hospital of Fudan University, No. 399, Wanyuan Road, Shanghai 201102, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, No. 399, Wanyuan Road, Shanghai 201102, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, No. 399, Wanyuan Road, Shanghai 201102, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, No. 399, Wanyuan Road, Shanghai 201102, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, No. 399, Wanyuan Road, Shanghai 201102, ChinaDepartment of Traditional Chinese Medicine, Children’s Hospital of Fudan University, No. 399, Wanyuan Road, Shanghai 201102, ChinaObjective. To observe the effects of empirical prescriptions of traditional Chinese medicine (TCM) on inflammatory mediators in pediatric asthma and to explore the underlying molecular mechanism in the treatment of asthma. Methods. A total of 182 children with asthma were randomly placed into either the TCM group (n=97) or the salbutamol and montelukast (SM) group (n=85). Patients in the TCM group were treated with a series of empirical prescriptions of TCM, while those in the SM group received salbutamol and montelukast. Both groups received their respective treatment for 12 weeks. There were 35 patients in TCM group and 34 patients in SM group providing venous blood. Real-time PCR was used to determine the mRNA expression levels of interleukin- (IL-) 10, IL-17, matrix metalloproteinase-9 (MMP-9), and transforming growth factor β1 (TGF-β1) in peripheral blood mononuclear cells before and after treatment. Enzyme-linked immunosorbent assay was used to measure the levels of IL-10, IL-17, MMP-9, and TGF-β1 in peripheral blood before and after treatment. Results. The mRNA expression of TGF-β1 in the SM group was downregulated (P=0.00) after treatment. No significant differences were found between the TCM group and the SM group after treatment (P>0.05). In the TCM group, the levels of IL-10, IL-17, and MMP-9 significantly decreased after treatment (P=0.01, 0.04, and 0.03, resp.). In the SM group, IL-17, MMP-9, and TGF-β1 levels significantly decreased after treatment (P=0.00, 0.03, and 0.00, resp.). There was no significant difference between the two groups regarding the levels of IL-10, IL-17, TGF-β1, and MMP-9 (P>0.05). The difference of the level of IL-17 was negatively correlated with the change of C-ACT score in TCM group and SM group. Conclusion. TCM has a regulatory effect on the balance of some inflammatory mediators in pediatric asthma.http://dx.doi.org/10.1155/2016/5143703 |
| spellingShingle | Hui Du Yonghong Wang Yumin Shi Jian Yu Wen Sun Yiqun Zhang Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma Mediators of Inflammation |
| title | Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma |
| title_full | Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma |
| title_fullStr | Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma |
| title_full_unstemmed | Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma |
| title_short | Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma |
| title_sort | effect of traditional chinese medicine on inflammatory mediators in pediatric asthma |
| url | http://dx.doi.org/10.1155/2016/5143703 |
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