Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model
IntroductionMarfan Syndrome (MFS) is a connective tissue disorder due to mutations in fibrillin-1 (Fbn1), where a Fbn1 missense mutation (Fbn1C1039G/+) can result in systemic increases in the bioavailability and signaling of transforming growth factor-β (TGF-β). In a well-established mouse model of...
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Frontiers Media S.A.
2025-01-01
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| author | Tala Curry-Koski Tala Curry-Koski Liam P. Curtin Mitra Esfandiarei Mitra Esfandiarei Mitra Esfandiarei Theresa Currier Thomas Theresa Currier Thomas Theresa Currier Thomas Theresa Currier Thomas |
| author_facet | Tala Curry-Koski Tala Curry-Koski Liam P. Curtin Mitra Esfandiarei Mitra Esfandiarei Mitra Esfandiarei Theresa Currier Thomas Theresa Currier Thomas Theresa Currier Thomas Theresa Currier Thomas |
| author_sort | Tala Curry-Koski |
| collection | DOAJ |
| description | IntroductionMarfan Syndrome (MFS) is a connective tissue disorder due to mutations in fibrillin-1 (Fbn1), where a Fbn1 missense mutation (Fbn1C1039G/+) can result in systemic increases in the bioavailability and signaling of transforming growth factor-β (TGF-β). In a well-established mouse model of MFS (Fbn1C1041G/+), pre-mature aging of the aortic wall and the progression of aortic root aneurysm are observed by 6-month-of-age. TGF-β signaling has been implicated in cerebrovascular dysfunction, loss of blood-brain barrier (BBB) integrity, and age-related neuroinflammation. We have reported that pre-mature vascular aging in MFS mice could extend to cerebrovasculature, where peak blood flow velocity in the posterior cerebral artery (PCA) of 6-month-old (6M) MFS mice was reduced, similarly to 12-month-old (12M) control mice. Case studies of MFS patients have documented neurovascular manifestations, including intracranial aneurysms, stroke, arterial tortuosity, as well as headaches and migraines, with reported incidences of pain and chronic fatigue. Despite these significant clinical observations, investigation into cerebrovascular dysfunction and neuropathology in MFS remains limited.MethodsUsing 6M-control (C57BL/6) and 6M-MFS (Fbn1C1041G/+) and healthy 12M-control male and female mice, we test the hypothesis that abnormal Fbn1 protein expression is associated with altered cerebral microvascular density, BBB permeability, and neuroinflammation in the PCA-perfused hippocampus, all indicative of a pre-mature aging brain phenotype. Glut1 immunostaining was used to quantify microvascular density, IgG staining to assess BBB permeability, and microglial counts to evaluate neuroinflammation.ResultsUsing Glut1 staining, 6M-MFS mice and 12M-CTRL similarly present decreased microvascular density in the dentate gyrus (DG), cornu ammonis 1 (CA1), and cornu ammonis 3 (CA3) regions of the hippocampus. 6M-MFS mice exhibit increased BBB permeability in the DG and CA3 as evident by Immunoglobulin G (IgG) staining. No differences were detected between 6M and 12M-CTRL mice. 6M-MFS mice show a higher number of microglia in the hippocampus compared to age-matched control mice, a pattern resembling that of 12M-CTRL mice.DiscussionThis study represents the first known investigation into neuropathology in a mouse model of MFS and indicates that the pathophysiology underlying MFS leads to a systemic pre-mature aging phenotype. This study is crucial for identifying and understanding MFS-associated neurovascular and neurological abnormalities, underscoring the need for research aimed at improving the quality of life and managing pre-mature aging symptoms in MFS and related connective tissue disorders. |
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| institution | Kabale University |
| issn | 1664-042X |
| language | English |
| publishDate | 2025-01-01 |
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| series | Frontiers in Physiology |
| spelling | doaj-art-06024e03c6a74313bf42501fde6875092025-01-31T06:40:11ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2025-01-011510.3389/fphys.2024.14570341457034Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse modelTala Curry-Koski0Tala Curry-Koski1Liam P. Curtin2Mitra Esfandiarei3Mitra Esfandiarei4Mitra Esfandiarei5Theresa Currier Thomas6Theresa Currier Thomas7Theresa Currier Thomas8Theresa Currier Thomas9Phoenix Children’s Research Institute, Department of Child Health, College of Medicine-Phoenix, University of Arizona, Phoenix, AZ, United StatesTranslational Neurotrauma and Neurochemistry Laboratory, Barrow Neurological Institute at Phoenix Children’s Hospital, Phoenix, AZ, United StatesCollege of Graduate Studies, Midwestern University, Glendale, AZ, United StatesPhoenix Children’s Research Institute, Department of Child Health, College of Medicine-Phoenix, University of Arizona, Phoenix, AZ, United StatesCollege of Graduate Studies, Midwestern University, Glendale, AZ, United StatesFaculty of Medicine, University of British Columbia, Vancouver, BC, CanadaPhoenix Children’s Research Institute, Department of Child Health, College of Medicine-Phoenix, University of Arizona, Phoenix, AZ, United StatesTranslational Neurotrauma and Neurochemistry Laboratory, Barrow Neurological Institute at Phoenix Children’s Hospital, Phoenix, AZ, United StatesCollege of Graduate Studies, Midwestern University, Glendale, AZ, United StatesPhoenix VA Healthcare System, Phoenix, AZ, United StatesIntroductionMarfan Syndrome (MFS) is a connective tissue disorder due to mutations in fibrillin-1 (Fbn1), where a Fbn1 missense mutation (Fbn1C1039G/+) can result in systemic increases in the bioavailability and signaling of transforming growth factor-β (TGF-β). In a well-established mouse model of MFS (Fbn1C1041G/+), pre-mature aging of the aortic wall and the progression of aortic root aneurysm are observed by 6-month-of-age. TGF-β signaling has been implicated in cerebrovascular dysfunction, loss of blood-brain barrier (BBB) integrity, and age-related neuroinflammation. We have reported that pre-mature vascular aging in MFS mice could extend to cerebrovasculature, where peak blood flow velocity in the posterior cerebral artery (PCA) of 6-month-old (6M) MFS mice was reduced, similarly to 12-month-old (12M) control mice. Case studies of MFS patients have documented neurovascular manifestations, including intracranial aneurysms, stroke, arterial tortuosity, as well as headaches and migraines, with reported incidences of pain and chronic fatigue. Despite these significant clinical observations, investigation into cerebrovascular dysfunction and neuropathology in MFS remains limited.MethodsUsing 6M-control (C57BL/6) and 6M-MFS (Fbn1C1041G/+) and healthy 12M-control male and female mice, we test the hypothesis that abnormal Fbn1 protein expression is associated with altered cerebral microvascular density, BBB permeability, and neuroinflammation in the PCA-perfused hippocampus, all indicative of a pre-mature aging brain phenotype. Glut1 immunostaining was used to quantify microvascular density, IgG staining to assess BBB permeability, and microglial counts to evaluate neuroinflammation.ResultsUsing Glut1 staining, 6M-MFS mice and 12M-CTRL similarly present decreased microvascular density in the dentate gyrus (DG), cornu ammonis 1 (CA1), and cornu ammonis 3 (CA3) regions of the hippocampus. 6M-MFS mice exhibit increased BBB permeability in the DG and CA3 as evident by Immunoglobulin G (IgG) staining. No differences were detected between 6M and 12M-CTRL mice. 6M-MFS mice show a higher number of microglia in the hippocampus compared to age-matched control mice, a pattern resembling that of 12M-CTRL mice.DiscussionThis study represents the first known investigation into neuropathology in a mouse model of MFS and indicates that the pathophysiology underlying MFS leads to a systemic pre-mature aging phenotype. This study is crucial for identifying and understanding MFS-associated neurovascular and neurological abnormalities, underscoring the need for research aimed at improving the quality of life and managing pre-mature aging symptoms in MFS and related connective tissue disorders.https://www.frontiersin.org/articles/10.3389/fphys.2024.1457034/fullMarfan syndromemicrovascular densityblood-brain barrierneuroinflammationneuropathologypremature aging |
| spellingShingle | Tala Curry-Koski Tala Curry-Koski Liam P. Curtin Mitra Esfandiarei Mitra Esfandiarei Mitra Esfandiarei Theresa Currier Thomas Theresa Currier Thomas Theresa Currier Thomas Theresa Currier Thomas Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model Frontiers in Physiology Marfan syndrome microvascular density blood-brain barrier neuroinflammation neuropathology premature aging |
| title | Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model |
| title_full | Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model |
| title_fullStr | Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model |
| title_full_unstemmed | Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model |
| title_short | Cerebral microvascular density, blood-brain barrier permeability, and support for neuroinflammation indicate early aging in a Marfan syndrome mouse model |
| title_sort | cerebral microvascular density blood brain barrier permeability and support for neuroinflammation indicate early aging in a marfan syndrome mouse model |
| topic | Marfan syndrome microvascular density blood-brain barrier neuroinflammation neuropathology premature aging |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2024.1457034/full |
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