Lipidated DAPEG Polymers as a Non-Toxic Transfection Agent—Influence of Fatty Acid Side Chain on Transfection Efficacy
This study describes the synthesis, interaction with DNA, and transfection efficacy of eight lipidated compounds based on a recently published non-lipidated parent molecule, an octamer of 2,3-<span style="font-variant: small-caps;">l</span>-Dap, carrying the guanidine group on...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/30/7/1644 |
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| author | Wiktoria Mallek Anita Romanowska Wiktoria Machowicz Agnieszka Piwkowska Adam Lesner Magdalena Wysocka |
| author_facet | Wiktoria Mallek Anita Romanowska Wiktoria Machowicz Agnieszka Piwkowska Adam Lesner Magdalena Wysocka |
| author_sort | Wiktoria Mallek |
| collection | DOAJ |
| description | This study describes the synthesis, interaction with DNA, and transfection efficacy of eight lipidated compounds based on a recently published non-lipidated parent molecule, an octamer of 2,3-<span style="font-variant: small-caps;">l</span>-Dap, carrying the guanidine group on its side chain. The compounds obtained were found to be non-toxic up to 5 µM and efficient DNA binders and showed greater transfection efficiency than the parent compound, with two leading molecules containing acetic and decanoic moieties. DLS experiments indicated two groups of interaction with DNA. One group modified by short-chain lipids (up to eight carbon atoms in the main chain) forms large structures due to the aggregation of multiple nucleic acids. The second group (from twelve to sixteen carbon atoms) with dominant condensation creates smaller forms and is less effective in transporting DNA into the cells. |
| format | Article |
| id | doaj-art-05eedbbcd89948bcbd4eab643bfb37d5 |
| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-05eedbbcd89948bcbd4eab643bfb37d52025-08-20T02:17:01ZengMDPI AGMolecules1420-30492025-04-01307164410.3390/molecules30071644Lipidated DAPEG Polymers as a Non-Toxic Transfection Agent—Influence of Fatty Acid Side Chain on Transfection EfficacyWiktoria Mallek0Anita Romanowska1Wiktoria Machowicz2Agnieszka Piwkowska3Adam Lesner4Magdalena Wysocka5Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, PolandFaculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, PolandFaculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, PolandMossakowski Medical Research Institute Polish Academy of Sciences, Wita Stwosza 63, 80-308 Gdansk, PolandFaculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, PolandFaculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, PolandThis study describes the synthesis, interaction with DNA, and transfection efficacy of eight lipidated compounds based on a recently published non-lipidated parent molecule, an octamer of 2,3-<span style="font-variant: small-caps;">l</span>-Dap, carrying the guanidine group on its side chain. The compounds obtained were found to be non-toxic up to 5 µM and efficient DNA binders and showed greater transfection efficiency than the parent compound, with two leading molecules containing acetic and decanoic moieties. DLS experiments indicated two groups of interaction with DNA. One group modified by short-chain lipids (up to eight carbon atoms in the main chain) forms large structures due to the aggregation of multiple nucleic acids. The second group (from twelve to sixteen carbon atoms) with dominant condensation creates smaller forms and is less effective in transporting DNA into the cells.https://www.mdpi.com/1420-3049/30/7/1644peptidomimeticstransfectionlipidated polymers |
| spellingShingle | Wiktoria Mallek Anita Romanowska Wiktoria Machowicz Agnieszka Piwkowska Adam Lesner Magdalena Wysocka Lipidated DAPEG Polymers as a Non-Toxic Transfection Agent—Influence of Fatty Acid Side Chain on Transfection Efficacy Molecules peptidomimetics transfection lipidated polymers |
| title | Lipidated DAPEG Polymers as a Non-Toxic Transfection Agent—Influence of Fatty Acid Side Chain on Transfection Efficacy |
| title_full | Lipidated DAPEG Polymers as a Non-Toxic Transfection Agent—Influence of Fatty Acid Side Chain on Transfection Efficacy |
| title_fullStr | Lipidated DAPEG Polymers as a Non-Toxic Transfection Agent—Influence of Fatty Acid Side Chain on Transfection Efficacy |
| title_full_unstemmed | Lipidated DAPEG Polymers as a Non-Toxic Transfection Agent—Influence of Fatty Acid Side Chain on Transfection Efficacy |
| title_short | Lipidated DAPEG Polymers as a Non-Toxic Transfection Agent—Influence of Fatty Acid Side Chain on Transfection Efficacy |
| title_sort | lipidated dapeg polymers as a non toxic transfection agent influence of fatty acid side chain on transfection efficacy |
| topic | peptidomimetics transfection lipidated polymers |
| url | https://www.mdpi.com/1420-3049/30/7/1644 |
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