An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol
The chemical composition and antibacterial activity of Thymus glabrescens Willd. (Lamiaceae) essential oil were examined, as well as the association between it and chloramphenicol. The antibacterial activities of geraniol and thymol, the main constituents of T. glabrescens oil, individually and in c...
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/826219 |
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| author | Budimir S. Ilić Branislava D. Kocić Vojislav M. Ćirić Olga G. Cvetković Dragoljub L. Miladinović |
| author_facet | Budimir S. Ilić Branislava D. Kocić Vojislav M. Ćirić Olga G. Cvetković Dragoljub L. Miladinović |
| author_sort | Budimir S. Ilić |
| collection | DOAJ |
| description | The chemical composition and antibacterial activity of Thymus glabrescens Willd. (Lamiaceae) essential oil were examined, as well as the association between it and chloramphenicol. The antibacterial activities of geraniol and thymol, the main constituents of T. glabrescens oil, individually and in combination with chloramphenicol, were also determined. The interactions of the essential oil, geraniol, and thymol with chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods. Oxygenated monoterpenes were the most abundant compound class in the oil, with geraniol (22.33%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol. A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21–0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects. The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol. |
| format | Article |
| id | doaj-art-05de32a74c3b434f9fd04ee896a5726a |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-05de32a74c3b434f9fd04ee896a5726a2025-02-03T01:32:53ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/826219826219An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with ChloramphenicolBudimir S. Ilić0Branislava D. Kocić1Vojislav M. Ćirić2Olga G. Cvetković3Dragoljub L. Miladinović4Department of Pharmacy, Faculty of Medicine, University of Niš, 18000 Niš, SerbiaCenter for Microbiology, Institute for Public Health, 18000 Niš, SerbiaClinic for Endocrinology, Diabetes and Diseases of Metabolism, Clinical Center Niš, 18000 Niš, SerbiaCenter of Chemistry, ICTM, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Pharmacy, Faculty of Medicine, University of Niš, 18000 Niš, SerbiaThe chemical composition and antibacterial activity of Thymus glabrescens Willd. (Lamiaceae) essential oil were examined, as well as the association between it and chloramphenicol. The antibacterial activities of geraniol and thymol, the main constituents of T. glabrescens oil, individually and in combination with chloramphenicol, were also determined. The interactions of the essential oil, geraniol, and thymol with chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods. Oxygenated monoterpenes were the most abundant compound class in the oil, with geraniol (22.33%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotic and thymol. A combination of T. glabrescens oil and chloramphenicol produced a strong synergistic interaction (FIC indices in the range 0.21–0.87) and a substantial reduction of the MIC value of chloramphenicol, thus minimizing its adverse side effects. The combinations geraniol-chloramphenicol and thymol-chloramphenicol produced synergistic interaction to a greater extent, compared with essential oil-chloramphenicol association, which may indicate that the activity of the thyme oil could be attributed to the presence of significant concentrations of geraniol and thymol.http://dx.doi.org/10.1155/2014/826219 |
| spellingShingle | Budimir S. Ilić Branislava D. Kocić Vojislav M. Ćirić Olga G. Cvetković Dragoljub L. Miladinović An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol The Scientific World Journal |
| title | An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol |
| title_full | An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol |
| title_fullStr | An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol |
| title_full_unstemmed | An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol |
| title_short | An In Vitro Synergistic Interaction of Combinations of Thymus glabrescens Essential Oil and Its Main Constituents with Chloramphenicol |
| title_sort | in vitro synergistic interaction of combinations of thymus glabrescens essential oil and its main constituents with chloramphenicol |
| url | http://dx.doi.org/10.1155/2014/826219 |
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