The Regulatory Effect of Se-Cd Interaction on Tea Plants (<i>Camellia sinensis</i> (L.) O. Kuntze) Under Cadmium Stress
This study utilized annual Fuding Dabaicha cuttings as the experimental subject, employing a nutrient solution cultivation technique to establish three distinct treatments: (1) CK: reference processing; (2) Cd: 20 mg/L CdSO<sub>4</sub> nutrient solution culture; (3) Cd + Se: 20 mg/L CdSO...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/15/1/246 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589375224938496 |
|---|---|
| author | Yanyun Sun Yueling Zhao Hongyu Zhou Faxing Li Yuanyuan Wang Xiao Du |
| author_facet | Yanyun Sun Yueling Zhao Hongyu Zhou Faxing Li Yuanyuan Wang Xiao Du |
| author_sort | Yanyun Sun |
| collection | DOAJ |
| description | This study utilized annual Fuding Dabaicha cuttings as the experimental subject, employing a nutrient solution cultivation technique to establish three distinct treatments: (1) CK: reference processing; (2) Cd: 20 mg/L CdSO<sub>4</sub> nutrient solution culture; (3) Cd + Se: 20 mg/L CdSO<sub>4</sub> + 1.5 mg/L Na<sub>2</sub>SeO<sub>3</sub> nutrient solution culture. We measured and analyzed the electrolyte permeability, proline content, malondialdehyde (MDA) content, antioxidant-related indicators, photosynthesis-related indicators, Cd content in various organs, and transmission electron microscopy images depicting the distribution of Cd and Se elements within different organs of tea plants under these treatments after 30 days of processing, studying the regulatory role of selenium on the resistance mechanism of tea plants (<i>Camellia sinensis</i>) under cadmium stress. The findings are as follows: (1) Cd stress notably elevated the electrical conductivity, MDA content, and proline content of tea plants, whereas the Cd + Se<sub>1</sub> treatment significantly reduced the MDA and proline content under Cd stress; (2) the Cd stress treatment significantly increased the content of peroxides in the tea tree leaves and significantly decreased the activities of four antioxidant enzymes, SOD, POD, CAT, and Apx; the Cd + Se treatment significantly reduced the peroxide content in tea trees under cadmium stress and significantly increased the activities of SOD, POD, CAT, and Apx; (3) the Cd stress treatment significantly increased the values of certain chlorophyll fluorescence parameters and had no significant impact on the distribution of light energy, whereas the Cd + Se treatment significantly elevated the values of some chlorophyll fluorescence parameters and induced an uneven distribution of light energy; (4) the order of accumulation of Cd in different organs of the tea plants was as follows: root > stem > leaf, and the Cd + Se treatment significantly reduced the Cd content in various organs under Cd stress. In root cells, Cd and Se were predominantly located in the cell wall, plasma membrane, and vacuole membrane; in stem cells, they were primarily found in the cell wall and cytoplasm adjacent to the cell wall; in leaf cells, they were mainly distributed in the plasma membrane, cytoplasm, and vacuole. In conclusion, Cd treatment induced stress in tea plants, which resulted in a certain stimulatory effect on photosynthesis, but caused some damage to the photosynthetic apparatus in chloroplasts. The results of the Cd + Se treatment suggest that the interaction between Se and Cd can mitigate the toxicity experienced by tea plants under Cd stress. |
| format | Article |
| id | doaj-art-05b08953591b4065a1aa03a1dfbc5271 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-05b08953591b4065a1aa03a1dfbc52712025-01-24T13:17:19ZengMDPI AGAgronomy2073-43952025-01-0115124610.3390/agronomy15010246The Regulatory Effect of Se-Cd Interaction on Tea Plants (<i>Camellia sinensis</i> (L.) O. Kuntze) Under Cadmium StressYanyun Sun0Yueling Zhao1Hongyu Zhou2Faxing Li3Yuanyuan Wang4Xiao Du5College of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaThis study utilized annual Fuding Dabaicha cuttings as the experimental subject, employing a nutrient solution cultivation technique to establish three distinct treatments: (1) CK: reference processing; (2) Cd: 20 mg/L CdSO<sub>4</sub> nutrient solution culture; (3) Cd + Se: 20 mg/L CdSO<sub>4</sub> + 1.5 mg/L Na<sub>2</sub>SeO<sub>3</sub> nutrient solution culture. We measured and analyzed the electrolyte permeability, proline content, malondialdehyde (MDA) content, antioxidant-related indicators, photosynthesis-related indicators, Cd content in various organs, and transmission electron microscopy images depicting the distribution of Cd and Se elements within different organs of tea plants under these treatments after 30 days of processing, studying the regulatory role of selenium on the resistance mechanism of tea plants (<i>Camellia sinensis</i>) under cadmium stress. The findings are as follows: (1) Cd stress notably elevated the electrical conductivity, MDA content, and proline content of tea plants, whereas the Cd + Se<sub>1</sub> treatment significantly reduced the MDA and proline content under Cd stress; (2) the Cd stress treatment significantly increased the content of peroxides in the tea tree leaves and significantly decreased the activities of four antioxidant enzymes, SOD, POD, CAT, and Apx; the Cd + Se treatment significantly reduced the peroxide content in tea trees under cadmium stress and significantly increased the activities of SOD, POD, CAT, and Apx; (3) the Cd stress treatment significantly increased the values of certain chlorophyll fluorescence parameters and had no significant impact on the distribution of light energy, whereas the Cd + Se treatment significantly elevated the values of some chlorophyll fluorescence parameters and induced an uneven distribution of light energy; (4) the order of accumulation of Cd in different organs of the tea plants was as follows: root > stem > leaf, and the Cd + Se treatment significantly reduced the Cd content in various organs under Cd stress. In root cells, Cd and Se were predominantly located in the cell wall, plasma membrane, and vacuole membrane; in stem cells, they were primarily found in the cell wall and cytoplasm adjacent to the cell wall; in leaf cells, they were mainly distributed in the plasma membrane, cytoplasm, and vacuole. In conclusion, Cd treatment induced stress in tea plants, which resulted in a certain stimulatory effect on photosynthesis, but caused some damage to the photosynthetic apparatus in chloroplasts. The results of the Cd + Se treatment suggest that the interaction between Se and Cd can mitigate the toxicity experienced by tea plants under Cd stress.https://www.mdpi.com/2073-4395/15/1/246<i>Camellia sinensis</i>cadmium stressSe-Cd interaction effectantioxidantphotosynthesision microdomain distribution |
| spellingShingle | Yanyun Sun Yueling Zhao Hongyu Zhou Faxing Li Yuanyuan Wang Xiao Du The Regulatory Effect of Se-Cd Interaction on Tea Plants (<i>Camellia sinensis</i> (L.) O. Kuntze) Under Cadmium Stress Agronomy <i>Camellia sinensis</i> cadmium stress Se-Cd interaction effect antioxidant photosynthesis ion microdomain distribution |
| title | The Regulatory Effect of Se-Cd Interaction on Tea Plants (<i>Camellia sinensis</i> (L.) O. Kuntze) Under Cadmium Stress |
| title_full | The Regulatory Effect of Se-Cd Interaction on Tea Plants (<i>Camellia sinensis</i> (L.) O. Kuntze) Under Cadmium Stress |
| title_fullStr | The Regulatory Effect of Se-Cd Interaction on Tea Plants (<i>Camellia sinensis</i> (L.) O. Kuntze) Under Cadmium Stress |
| title_full_unstemmed | The Regulatory Effect of Se-Cd Interaction on Tea Plants (<i>Camellia sinensis</i> (L.) O. Kuntze) Under Cadmium Stress |
| title_short | The Regulatory Effect of Se-Cd Interaction on Tea Plants (<i>Camellia sinensis</i> (L.) O. Kuntze) Under Cadmium Stress |
| title_sort | regulatory effect of se cd interaction on tea plants i camellia sinensis i l o kuntze under cadmium stress |
| topic | <i>Camellia sinensis</i> cadmium stress Se-Cd interaction effect antioxidant photosynthesis ion microdomain distribution |
| url | https://www.mdpi.com/2073-4395/15/1/246 |
| work_keys_str_mv | AT yanyunsun theregulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT yuelingzhao theregulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT hongyuzhou theregulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT faxingli theregulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT yuanyuanwang theregulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT xiaodu theregulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT yanyunsun regulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT yuelingzhao regulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT hongyuzhou regulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT faxingli regulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT yuanyuanwang regulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress AT xiaodu regulatoryeffectofsecdinteractiononteaplantsicamelliasinensisilokuntzeundercadmiumstress |