Isolation and full genome sequencing of two human Astroviruses isolated from children in Cairo, Egypt
Astroviridae is a diverse family of viruses that are circulating in a wide range of hosts. Diversity of these viruses plays a major role in the emergence of zoonotic diseases. Limited studies were conducted on the prevalence and genetic proprieties of human Astroviruses in Egypt. Despite the molec...
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ResearchersLinks, Ltd
2020-02-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_73445_9c0f0a458041d339bc40cb59b1e9bdae.pdf |
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| author | Ahmed El Taweel Ahmed Kandeil Ahmed Barakat Omar El Faroq Ghazi Kayali Mohamed Ahmed Ali |
| author_facet | Ahmed El Taweel Ahmed Kandeil Ahmed Barakat Omar El Faroq Ghazi Kayali Mohamed Ahmed Ali |
| author_sort | Ahmed El Taweel |
| collection | DOAJ |
| description | Astroviridae is a diverse family of viruses that are circulating in a wide range of hosts. Diversity of these
viruses plays a major role in the emergence of zoonotic diseases. Limited studies were conducted on the
prevalence and genetic proprieties of human Astroviruses in Egypt. Despite the molecular and serological
evidences of the existence human astroviruses in several regions of Egypt, attempts to isolate these viruses have
been largely unsuccessful. The aims of the current study were to isolate and make full genome sequencing of two
human Astroviruses genotype 1 and 4, from infected children in Cairo, Egypt. Currently, we report the isolation
and full-genome sequencing of two human isolates based on our novel designed overlapped primers. The original
stool samples were collected from children suffering from diarrhea, residing in Abo-El-Reesh Hospital, Cairo,
Egypt, and were well characterized as having astrovirus infections. The phylogenetic relationship of the obtained
sequences of our isolates collected in 2016, revealed that they are related to human astrovirus genotype 1
(HAstV1) and human astrovirus genotype 4 (HAstV4) isolates recovered from Italy and Russia; respectively,
during this decade. The genetic diversity of the other HAstVs circulating in Egypt needs to be fully identified, as
a first step toward the control strategy of this neglected virus. |
| format | Article |
| id | doaj-art-05ab4a85ae4b4ebfbf7e2fb3fd03cd89 |
| institution | Kabale University |
| issn | 2537-0286 2537-0294 |
| language | English |
| publishDate | 2020-02-01 |
| publisher | ResearchersLinks, Ltd |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj-art-05ab4a85ae4b4ebfbf7e2fb3fd03cd892025-08-20T03:54:28ZengResearchersLinks, LtdNovel Research in Microbiology Journal2537-02862537-02942020-02-014166667410.21608/nrmj.2020.73445Isolation and full genome sequencing of two human Astroviruses isolated from children in Cairo, EgyptAhmed El Taweel0Ahmed Kandeil1Ahmed Barakat2Omar El Faroq3Ghazi Kayali4 Mohamed Ahmed Ali5Center of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, El-Buhouth Street, Dokki, Giza 12311, EgyptCenter of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, El-Buhouth Street, Dokki, Giza 12311, EgyptMicrobiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt;Microbiology Department, Faculty of Science, Ain Shams University, Cairo, EgyptHuman Link, Hazmieh, LebanonCenter of Scientific Excellence for Influenza Virus, Environmental Research Division, National Research Centre, El-Buhouth Street, Dokki, Giza 12311, EgyptAstroviridae is a diverse family of viruses that are circulating in a wide range of hosts. Diversity of these viruses plays a major role in the emergence of zoonotic diseases. Limited studies were conducted on the prevalence and genetic proprieties of human Astroviruses in Egypt. Despite the molecular and serological evidences of the existence human astroviruses in several regions of Egypt, attempts to isolate these viruses have been largely unsuccessful. The aims of the current study were to isolate and make full genome sequencing of two human Astroviruses genotype 1 and 4, from infected children in Cairo, Egypt. Currently, we report the isolation and full-genome sequencing of two human isolates based on our novel designed overlapped primers. The original stool samples were collected from children suffering from diarrhea, residing in Abo-El-Reesh Hospital, Cairo, Egypt, and were well characterized as having astrovirus infections. The phylogenetic relationship of the obtained sequences of our isolates collected in 2016, revealed that they are related to human astrovirus genotype 1 (HAstV1) and human astrovirus genotype 4 (HAstV4) isolates recovered from Italy and Russia; respectively, during this decade. The genetic diversity of the other HAstVs circulating in Egypt needs to be fully identified, as a first step toward the control strategy of this neglected virus.https://nrmj.journals.ekb.eg/article_73445_9c0f0a458041d339bc40cb59b1e9bdae.pdfastrovirusesegyptisolationsequencingphylogenetic analysis |
| spellingShingle | Ahmed El Taweel Ahmed Kandeil Ahmed Barakat Omar El Faroq Ghazi Kayali Mohamed Ahmed Ali Isolation and full genome sequencing of two human Astroviruses isolated from children in Cairo, Egypt Novel Research in Microbiology Journal astroviruses egypt isolation sequencing phylogenetic analysis |
| title | Isolation and full genome sequencing of two human Astroviruses isolated from children in Cairo, Egypt |
| title_full | Isolation and full genome sequencing of two human Astroviruses isolated from children in Cairo, Egypt |
| title_fullStr | Isolation and full genome sequencing of two human Astroviruses isolated from children in Cairo, Egypt |
| title_full_unstemmed | Isolation and full genome sequencing of two human Astroviruses isolated from children in Cairo, Egypt |
| title_short | Isolation and full genome sequencing of two human Astroviruses isolated from children in Cairo, Egypt |
| title_sort | isolation and full genome sequencing of two human astroviruses isolated from children in cairo egypt |
| topic | astroviruses egypt isolation sequencing phylogenetic analysis |
| url | https://nrmj.journals.ekb.eg/article_73445_9c0f0a458041d339bc40cb59b1e9bdae.pdf |
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