Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study
The molecular mechanism formation of secondary epiretinal membranes (ERMs) after proliferative diabetic retinopathy (PDR) or primary idiopathic ERMs is still poorly understood. Therefore, the present study focused on the assessment of IGF1, IGF1R, and IGFBP3 mRNA levels in ERMs and PBMCs from patien...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/986217 |
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| author | Dorota Romaniuk Malgorzata W. Kimsa Barbara Strzalka-Mrozik Magdalena C. Kimsa Adam Kabiesz Wanda Romaniuk Urszula Mazurek |
| author_facet | Dorota Romaniuk Malgorzata W. Kimsa Barbara Strzalka-Mrozik Magdalena C. Kimsa Adam Kabiesz Wanda Romaniuk Urszula Mazurek |
| author_sort | Dorota Romaniuk |
| collection | DOAJ |
| description | The molecular mechanism formation of secondary epiretinal membranes (ERMs) after proliferative diabetic retinopathy (PDR) or primary idiopathic ERMs is still poorly understood. Therefore, the present study focused on the assessment of IGF1, IGF1R, and IGFBP3 mRNA levels in ERMs and PBMCs from patients with PDR. The examined group comprised 6 patients with secondary ERMs after PDR and the control group consisted of 11 patients with idiopathic ERMs. Quantification of IGF1, IGF1R, and IGFBP3 mRNAs was performed by real-time QRT-PCR technique. In ERMs, IGF1 and IGF1R mRNA levels were significantly higher in patients with diabetes compared to control subjects. In PBMCs, there were no statistically significant differences of IGF1, IGF1R, and IGFBP3 expression between diabetic and nondiabetic patients. In conclusion, our study indicated IGF1 and IGF1R differential expression in ERMs, but not in PBMCs, of diabetic and nondiabetic patients, suggesting that these factors can be involved in the pathogenesis or progression of proliferative vitreoretinal disorders. This trial is registered with NCT00841334. |
| format | Article |
| id | doaj-art-05a27c64b30b4aa295b47a2b9028e6c6 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-05a27c64b30b4aa295b47a2b9028e6c62025-02-03T05:50:10ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/986217986217Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary StudyDorota Romaniuk0Malgorzata W. Kimsa1Barbara Strzalka-Mrozik2Magdalena C. Kimsa3Adam Kabiesz4Wanda Romaniuk5Urszula Mazurek6Clinical Department of Ophthalmology, Medical University of Silesia, Ceglana Street 35, 40-952 Katowice, PolandDepartment of Molecular Biology, Medical University of Silesia, Narcyzów Street 1, 41-200 Sosnowiec, PolandDepartment of Molecular Biology, Medical University of Silesia, Narcyzów Street 1, 41-200 Sosnowiec, PolandDepartment of Molecular Biology, Medical University of Silesia, Narcyzów Street 1, 41-200 Sosnowiec, PolandClinical Department of Ophthalmology, Medical University of Silesia, Ceglana Street 35, 40-952 Katowice, PolandClinical Department of Ophthalmology, Medical University of Silesia, Ceglana Street 35, 40-952 Katowice, PolandDepartment of Molecular Biology, Medical University of Silesia, Narcyzów Street 1, 41-200 Sosnowiec, PolandThe molecular mechanism formation of secondary epiretinal membranes (ERMs) after proliferative diabetic retinopathy (PDR) or primary idiopathic ERMs is still poorly understood. Therefore, the present study focused on the assessment of IGF1, IGF1R, and IGFBP3 mRNA levels in ERMs and PBMCs from patients with PDR. The examined group comprised 6 patients with secondary ERMs after PDR and the control group consisted of 11 patients with idiopathic ERMs. Quantification of IGF1, IGF1R, and IGFBP3 mRNAs was performed by real-time QRT-PCR technique. In ERMs, IGF1 and IGF1R mRNA levels were significantly higher in patients with diabetes compared to control subjects. In PBMCs, there were no statistically significant differences of IGF1, IGF1R, and IGFBP3 expression between diabetic and nondiabetic patients. In conclusion, our study indicated IGF1 and IGF1R differential expression in ERMs, but not in PBMCs, of diabetic and nondiabetic patients, suggesting that these factors can be involved in the pathogenesis or progression of proliferative vitreoretinal disorders. This trial is registered with NCT00841334.http://dx.doi.org/10.1155/2013/986217 |
| spellingShingle | Dorota Romaniuk Malgorzata W. Kimsa Barbara Strzalka-Mrozik Magdalena C. Kimsa Adam Kabiesz Wanda Romaniuk Urszula Mazurek Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study Mediators of Inflammation |
| title | Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study |
| title_full | Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study |
| title_fullStr | Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study |
| title_full_unstemmed | Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study |
| title_short | Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study |
| title_sort | gene expression of igf1 igf1r and igfbp3 in epiretinal membranes of patients with proliferative diabetic retinopathy preliminary study |
| url | http://dx.doi.org/10.1155/2013/986217 |
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