Single-cell sequencing of peripheral blood mononuclear cells reveals immune landscape of monkeypox patients with HIV
The monkeypox (MPXV) outbreak in 2022 is more prevalent among individuals with human immunodeficiency virus (HIV). While it is plausible that HIV-induced immunosuppression could result in a more severe progression, the exact mechanisms remain undetermined. To better understand the immunopathology of...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2025.2459136 |
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| author | Yamin Liu Xinhua Liu Jingjing Wang Ying Xie Jing Guo Zhiqiang Liu Ying Li Bei Jiang Jingya Wang |
| author_facet | Yamin Liu Xinhua Liu Jingjing Wang Ying Xie Jing Guo Zhiqiang Liu Ying Li Bei Jiang Jingya Wang |
| author_sort | Yamin Liu |
| collection | DOAJ |
| description | The monkeypox (MPXV) outbreak in 2022 is more prevalent among individuals with human immunodeficiency virus (HIV). While it is plausible that HIV-induced immunosuppression could result in a more severe progression, the exact mechanisms remain undetermined. To better understand the immunopathology of MPXV in patients with and without HIV infection, we employed single-cell RNA sequencing (scRNA-seq) to analyse peripheral blood mononuclear cells (PBMCs) from six patients hospitalized for MPXV, three of whom had HIV infection (HIV antibody positive and HIV RNA level below the detection limit), and three patients only infected with MPXV (HIV-). We map the peripheral immune response in both the acute phase and the recovery period, showing the reconfiguration of peripheral immune cell phenotypes in acute stage compared with recovery stage, characterized by disturbed cell subsets and intense cell interactions mediated by monocytes and neutrophils. Importantly, we also found obviously dysregulated gene expression and cell subsets in HIV+ patients proposing mechanism underlying their serious condition. Our findings provide a comprehensive cell atlas of MPXV patients, shed light on the mechanisms underlying the severe disease progression and longer recovery time in HIV+ individuals. |
| format | Article |
| id | doaj-art-059bece450e940e6b631658c69927d47 |
| institution | Kabale University |
| issn | 2222-1751 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-059bece450e940e6b631658c69927d472025-08-20T03:47:58ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512025-12-0114110.1080/22221751.2025.2459136Single-cell sequencing of peripheral blood mononuclear cells reveals immune landscape of monkeypox patients with HIVYamin Liu0Xinhua Liu1Jingjing Wang2Ying Xie3Jing Guo4Zhiqiang Liu5Ying Li6Bei Jiang7Jingya Wang8Tianjin Institute of Hepatology, Tianjin Second People's Hospital, Tianjin, People’s Republic of ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou, People’s Republic of ChinaShandong Provincial Key Laboratory of Precision Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, People's Republic of ChinaShandong Provincial Key Laboratory of Precision Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, People's Republic of ChinaShandong Provincial Key Laboratory of Precision Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, People's Republic of ChinaShandong Provincial Key Laboratory of Precision Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, People's Republic of ChinaTianjin Institute of Hepatology, Tianjin Second People's Hospital, Tianjin, People’s Republic of ChinaTianjin Institute of Hepatology, Tianjin Second People's Hospital, Tianjin, People’s Republic of ChinaState Key Laboratory of Experimental Hematology, Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin, People’s Republic of ChinaThe monkeypox (MPXV) outbreak in 2022 is more prevalent among individuals with human immunodeficiency virus (HIV). While it is plausible that HIV-induced immunosuppression could result in a more severe progression, the exact mechanisms remain undetermined. To better understand the immunopathology of MPXV in patients with and without HIV infection, we employed single-cell RNA sequencing (scRNA-seq) to analyse peripheral blood mononuclear cells (PBMCs) from six patients hospitalized for MPXV, three of whom had HIV infection (HIV antibody positive and HIV RNA level below the detection limit), and three patients only infected with MPXV (HIV-). We map the peripheral immune response in both the acute phase and the recovery period, showing the reconfiguration of peripheral immune cell phenotypes in acute stage compared with recovery stage, characterized by disturbed cell subsets and intense cell interactions mediated by monocytes and neutrophils. Importantly, we also found obviously dysregulated gene expression and cell subsets in HIV+ patients proposing mechanism underlying their serious condition. Our findings provide a comprehensive cell atlas of MPXV patients, shed light on the mechanisms underlying the severe disease progression and longer recovery time in HIV+ individuals.https://www.tandfonline.com/doi/10.1080/22221751.2025.2459136MonkeypoxHIVsingle-cell RNA sequencingimmune responseantiviral innate immunity |
| spellingShingle | Yamin Liu Xinhua Liu Jingjing Wang Ying Xie Jing Guo Zhiqiang Liu Ying Li Bei Jiang Jingya Wang Single-cell sequencing of peripheral blood mononuclear cells reveals immune landscape of monkeypox patients with HIV Emerging Microbes and Infections Monkeypox HIV single-cell RNA sequencing immune response antiviral innate immunity |
| title | Single-cell sequencing of peripheral blood mononuclear cells reveals immune landscape of monkeypox patients with HIV |
| title_full | Single-cell sequencing of peripheral blood mononuclear cells reveals immune landscape of monkeypox patients with HIV |
| title_fullStr | Single-cell sequencing of peripheral blood mononuclear cells reveals immune landscape of monkeypox patients with HIV |
| title_full_unstemmed | Single-cell sequencing of peripheral blood mononuclear cells reveals immune landscape of monkeypox patients with HIV |
| title_short | Single-cell sequencing of peripheral blood mononuclear cells reveals immune landscape of monkeypox patients with HIV |
| title_sort | single cell sequencing of peripheral blood mononuclear cells reveals immune landscape of monkeypox patients with hiv |
| topic | Monkeypox HIV single-cell RNA sequencing immune response antiviral innate immunity |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2025.2459136 |
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