Hepatoprotective Effects of <i>Glycyrrhiza glabra</i> in Diabetic Male Rats: Addressing Liver Function, Oxidative Stress, and Histopathological Changes

Diabetes mellitus is a chronic metabolic disorder that often leads to hepatic complications, including oxidative stress, inflammation, and structural damage, highlighting the need for effective hepatoprotective agents. <i>Glycyrrhiza glabra</i> (licorice), known for its bioactive compoun...

Full description

Saved in:
Bibliographic Details
Main Authors: Abdulmajeed F. Alrefaei, Mohamed E. Elbeeh
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Biology
Subjects:
Online Access:https://www.mdpi.com/2079-7737/14/3/307
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850089592058806272
author Abdulmajeed F. Alrefaei
Mohamed E. Elbeeh
author_facet Abdulmajeed F. Alrefaei
Mohamed E. Elbeeh
author_sort Abdulmajeed F. Alrefaei
collection DOAJ
description Diabetes mellitus is a chronic metabolic disorder that often leads to hepatic complications, including oxidative stress, inflammation, and structural damage, highlighting the need for effective hepatoprotective agents. <i>Glycyrrhiza glabra</i> (licorice), known for its bioactive compounds with medicinal properties, has gained attention as a potential therapeutic option. This study investigated its hepatoprotective effects in a diabetic rat model. Diabetes was induced using streptozotocin, and animals were divided into four groups: a healthy control, a non-diabetic group treated with <i>Glycyrrhiza glabra</i> extract, an untreated diabetic group, and a diabetic group receiving daily doses of the extract. Liver function was assessed through serum enzyme levels (ALT, AST, and ALP), while oxidative stress markers, including malondialdehyde (MDA) and glutathione (GSH), were analyzed. Histological examination was conducted to evaluate inflammation, fibrosis, and fatty degeneration. Treatment with <i>Glycyrrhiza glabra</i> significantly improved liver function in diabetic rats, as evidenced by lower ALT, AST, and ALP levels compared to untreated diabetic rats (<i>p</i> < 0.05), suggesting hepatocyte protection through its antioxidant and anti-inflammatory properties. Favorable changes in oxidative stress markers were observed, with lower MDA levels indicating reduced lipid peroxidation and higher GSH activity reflecting enhanced antioxidant defense. Histological analysis confirmed these protective effects, showing reduced inflammation, diminished fatty degeneration, and overall structural improvements. These findings highlight the hepatoprotective potential of <i>Glycyrrhiza glabra</i>, suggesting it may be a promising therapeutic agent for managing hepatic complications associated with diabetes mellitus, though further research with larger sample sizes and mechanistic studies is needed for confirmation.
format Article
id doaj-art-059ab70554ab49eba828756ed724fcb6
institution DOAJ
issn 2079-7737
language English
publishDate 2025-03-01
publisher MDPI AG
record_format Article
series Biology
spelling doaj-art-059ab70554ab49eba828756ed724fcb62025-08-20T02:42:45ZengMDPI AGBiology2079-77372025-03-0114330710.3390/biology14030307Hepatoprotective Effects of <i>Glycyrrhiza glabra</i> in Diabetic Male Rats: Addressing Liver Function, Oxidative Stress, and Histopathological ChangesAbdulmajeed F. Alrefaei0Mohamed E. Elbeeh1Department of Biology/Genetic and Molecular Biology Central Laboratory (GMCL), Jamoum University College, Umm Al-Qura University, Makkah 2203, Saudi ArabiaDepartment of Biology, Jamoum University College, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDiabetes mellitus is a chronic metabolic disorder that often leads to hepatic complications, including oxidative stress, inflammation, and structural damage, highlighting the need for effective hepatoprotective agents. <i>Glycyrrhiza glabra</i> (licorice), known for its bioactive compounds with medicinal properties, has gained attention as a potential therapeutic option. This study investigated its hepatoprotective effects in a diabetic rat model. Diabetes was induced using streptozotocin, and animals were divided into four groups: a healthy control, a non-diabetic group treated with <i>Glycyrrhiza glabra</i> extract, an untreated diabetic group, and a diabetic group receiving daily doses of the extract. Liver function was assessed through serum enzyme levels (ALT, AST, and ALP), while oxidative stress markers, including malondialdehyde (MDA) and glutathione (GSH), were analyzed. Histological examination was conducted to evaluate inflammation, fibrosis, and fatty degeneration. Treatment with <i>Glycyrrhiza glabra</i> significantly improved liver function in diabetic rats, as evidenced by lower ALT, AST, and ALP levels compared to untreated diabetic rats (<i>p</i> < 0.05), suggesting hepatocyte protection through its antioxidant and anti-inflammatory properties. Favorable changes in oxidative stress markers were observed, with lower MDA levels indicating reduced lipid peroxidation and higher GSH activity reflecting enhanced antioxidant defense. Histological analysis confirmed these protective effects, showing reduced inflammation, diminished fatty degeneration, and overall structural improvements. These findings highlight the hepatoprotective potential of <i>Glycyrrhiza glabra</i>, suggesting it may be a promising therapeutic agent for managing hepatic complications associated with diabetes mellitus, though further research with larger sample sizes and mechanistic studies is needed for confirmation.https://www.mdpi.com/2079-7737/14/3/307<i>Glycyrrhiza glabra</i>diabetes mellitushepatoprotectionoxidative stresshistopathology
spellingShingle Abdulmajeed F. Alrefaei
Mohamed E. Elbeeh
Hepatoprotective Effects of <i>Glycyrrhiza glabra</i> in Diabetic Male Rats: Addressing Liver Function, Oxidative Stress, and Histopathological Changes
Biology
<i>Glycyrrhiza glabra</i>
diabetes mellitus
hepatoprotection
oxidative stress
histopathology
title Hepatoprotective Effects of <i>Glycyrrhiza glabra</i> in Diabetic Male Rats: Addressing Liver Function, Oxidative Stress, and Histopathological Changes
title_full Hepatoprotective Effects of <i>Glycyrrhiza glabra</i> in Diabetic Male Rats: Addressing Liver Function, Oxidative Stress, and Histopathological Changes
title_fullStr Hepatoprotective Effects of <i>Glycyrrhiza glabra</i> in Diabetic Male Rats: Addressing Liver Function, Oxidative Stress, and Histopathological Changes
title_full_unstemmed Hepatoprotective Effects of <i>Glycyrrhiza glabra</i> in Diabetic Male Rats: Addressing Liver Function, Oxidative Stress, and Histopathological Changes
title_short Hepatoprotective Effects of <i>Glycyrrhiza glabra</i> in Diabetic Male Rats: Addressing Liver Function, Oxidative Stress, and Histopathological Changes
title_sort hepatoprotective effects of i glycyrrhiza glabra i in diabetic male rats addressing liver function oxidative stress and histopathological changes
topic <i>Glycyrrhiza glabra</i>
diabetes mellitus
hepatoprotection
oxidative stress
histopathology
url https://www.mdpi.com/2079-7737/14/3/307
work_keys_str_mv AT abdulmajeedfalrefaei hepatoprotectiveeffectsofiglycyrrhizaglabraiindiabeticmaleratsaddressingliverfunctionoxidativestressandhistopathologicalchanges
AT mohamedeelbeeh hepatoprotectiveeffectsofiglycyrrhizaglabraiindiabeticmaleratsaddressingliverfunctionoxidativestressandhistopathologicalchanges