Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urine
Human papillomavirus (HPV) screening is crucial for early diagnosis and prevention of cervical cancer, yet fast and convenient HPV detection remains challenging, especially in resource-limited areas. Herein, we developed a nucleic acid test named SSMG-LAMP, which combined loop-mediated isothermal am...
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| Language: | English |
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Elsevier
2025-10-01
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| Series: | Biosensors and Bioelectronics: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137025000858 |
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| author | Duc Anh Thai Jing Liu Angel Gutierrez Ruiz Yuqian Zhang Marina Walther-Antonio Yuguang Liu |
| author_facet | Duc Anh Thai Jing Liu Angel Gutierrez Ruiz Yuqian Zhang Marina Walther-Antonio Yuguang Liu |
| author_sort | Duc Anh Thai |
| collection | DOAJ |
| description | Human papillomavirus (HPV) screening is crucial for early diagnosis and prevention of cervical cancer, yet fast and convenient HPV detection remains challenging, especially in resource-limited areas. Herein, we developed a nucleic acid test named SSMG-LAMP, which combined loop-mediated isothermal amplification (LAMP) with an engineered DNA indicator (SYBR Safe – Malachite Green) for the point-of-care diagnosis of high-risk HPV strains (HPV 16 and 18) in urine. The assay can be completed within 45 min, including DNA extraction, SSMG-LAMP reaction, and signal readout using a simple, portable system. This system enabled the triple-mode detection of DNA targets using colorimetry, fluorometry, and electrochemistry, and can detect as low as 10 copies μL−1 HPV DNA. As a preliminary validation, we used SSMG-LAMP for a blind test of 16 clinical urine samples to detect HPV 16 and 18, and results showed a sensitivity of >80 % and specificity of up to 96.2 %, with a 95 % confidence interval. This triple-mode HPV detection strategy holds potential for point-of-care cervical cancer screening in low-resource settings. |
| format | Article |
| id | doaj-art-059270993b5341e58f494d5a2f1a6685 |
| institution | Kabale University |
| issn | 2590-1370 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj-art-059270993b5341e58f494d5a2f1a66852025-08-20T03:50:22ZengElsevierBiosensors and Bioelectronics: X2590-13702025-10-012610065810.1016/j.biosx.2025.100658Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urineDuc Anh Thai0Jing Liu1Angel Gutierrez Ruiz2Yuqian Zhang3Marina Walther-Antonio4Yuguang Liu5Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA; Microbiome Program, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USADepartment of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA; Microbiome Program, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USADepartment of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA; Department of Chemistry and Biochemistry, The University of Texas at El Paso, El Paso, TX, 79968, USADepartment of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA; Microbiome Program, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USAMicrobiome Program, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USA; Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, 55905, USA; Department of Surgery, Mayo Clinic, Rochester, MN, 55905, USADepartment of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA; Microbiome Program, Center for Individualized Medicine, Mayo Clinic, Rochester, MN, 55905, USA; Department of Surgery, Mayo Clinic, Rochester, MN, 55905, USA; Department of Immunology, Mayo Clinic, Rochester, MN, 55905, USA; Corresponding author. Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA.Human papillomavirus (HPV) screening is crucial for early diagnosis and prevention of cervical cancer, yet fast and convenient HPV detection remains challenging, especially in resource-limited areas. Herein, we developed a nucleic acid test named SSMG-LAMP, which combined loop-mediated isothermal amplification (LAMP) with an engineered DNA indicator (SYBR Safe – Malachite Green) for the point-of-care diagnosis of high-risk HPV strains (HPV 16 and 18) in urine. The assay can be completed within 45 min, including DNA extraction, SSMG-LAMP reaction, and signal readout using a simple, portable system. This system enabled the triple-mode detection of DNA targets using colorimetry, fluorometry, and electrochemistry, and can detect as low as 10 copies μL−1 HPV DNA. As a preliminary validation, we used SSMG-LAMP for a blind test of 16 clinical urine samples to detect HPV 16 and 18, and results showed a sensitivity of >80 % and specificity of up to 96.2 %, with a 95 % confidence interval. This triple-mode HPV detection strategy holds potential for point-of-care cervical cancer screening in low-resource settings.http://www.sciencedirect.com/science/article/pii/S2590137025000858Human papillomavirusCervical cancerLoop-mediated isothermal amplificationPoint-of-careTriple-mode detection |
| spellingShingle | Duc Anh Thai Jing Liu Angel Gutierrez Ruiz Yuqian Zhang Marina Walther-Antonio Yuguang Liu Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urine Biosensors and Bioelectronics: X Human papillomavirus Cervical cancer Loop-mediated isothermal amplification Point-of-care Triple-mode detection |
| title | Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urine |
| title_full | Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urine |
| title_fullStr | Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urine |
| title_full_unstemmed | Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urine |
| title_short | Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urine |
| title_sort | triple mode point of care diagnostics for high risk human papillomavirus in urine |
| topic | Human papillomavirus Cervical cancer Loop-mediated isothermal amplification Point-of-care Triple-mode detection |
| url | http://www.sciencedirect.com/science/article/pii/S2590137025000858 |
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