Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse
Background. We investigated the effects of loss of inducible nitric oxide synthase (iNOS) on the healing process of cutaneous excisional injury by using iNOS-null (KO) mice. Population of granulation tissue-related cell types, that is, myofibroblasts and macrophages, growth factor expression, and re...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Dermatology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2017/2184040 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561682864406528 |
|---|---|
| author | Takashi Kitano Hiroshi Yamada Maki Kida Yuka Okada Shizuya Saika Munehito Yoshida |
| author_facet | Takashi Kitano Hiroshi Yamada Maki Kida Yuka Okada Shizuya Saika Munehito Yoshida |
| author_sort | Takashi Kitano |
| collection | DOAJ |
| description | Background. We investigated the effects of loss of inducible nitric oxide synthase (iNOS) on the healing process of cutaneous excisional injury by using iNOS-null (KO) mice. Population of granulation tissue-related cell types, that is, myofibroblasts and macrophages, growth factor expression, and reepithelialization were evaluated. Methods. KO and wild type (WT) mice of C57BL/6 background were used. Under general anesthesia two round full-thickness excision wounds of 5.0 mm in diameter were produced in dorsal skin. After specific intervals of healing, macroscopic observation, histology, immunohistochemistry, and real-time reverse transcription-polymerase chain reaction (RT-PCR) were employed to evaluate the healing process. Results. The loss of iNOS retards granulation tissue formation and reepithelialization in excision wound model in mice. Detailed analyses showed that myofibroblast appearance, macrophage infiltration, and mRNA expression of transforming growth factor b and of collagen 1α2 were all suppressed by lacking iNOS. Conclusions. iNOS is required in the process of cutaneous wound healing. Lacking iNOS retards macrophage invasion and its expression of fibrogenic components that might further impair fibrogenic behaviors of fibroblasts. |
| format | Article |
| id | doaj-art-058afba3f0ea43a1b737c6efceeee56f |
| institution | Kabale University |
| issn | 1687-6105 1687-6113 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Dermatology Research and Practice |
| spelling | doaj-art-058afba3f0ea43a1b737c6efceeee56f2025-02-03T01:24:21ZengWileyDermatology Research and Practice1687-61051687-61132017-01-01201710.1155/2017/21840402184040Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout MouseTakashi Kitano0Hiroshi Yamada1Maki Kida2Yuka Okada3Shizuya Saika4Munehito Yoshida5Department of Orthopedic Surgery, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-0012, JapanDepartment of Orthopedic Surgery, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-0012, JapanDepartment of Ophthalmology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-0012, JapanDepartment of Critical Care Medicine, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-0012, JapanDepartment of Critical Care Medicine, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-0012, JapanDepartment of Orthopedic Surgery, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-0012, JapanBackground. We investigated the effects of loss of inducible nitric oxide synthase (iNOS) on the healing process of cutaneous excisional injury by using iNOS-null (KO) mice. Population of granulation tissue-related cell types, that is, myofibroblasts and macrophages, growth factor expression, and reepithelialization were evaluated. Methods. KO and wild type (WT) mice of C57BL/6 background were used. Under general anesthesia two round full-thickness excision wounds of 5.0 mm in diameter were produced in dorsal skin. After specific intervals of healing, macroscopic observation, histology, immunohistochemistry, and real-time reverse transcription-polymerase chain reaction (RT-PCR) were employed to evaluate the healing process. Results. The loss of iNOS retards granulation tissue formation and reepithelialization in excision wound model in mice. Detailed analyses showed that myofibroblast appearance, macrophage infiltration, and mRNA expression of transforming growth factor b and of collagen 1α2 were all suppressed by lacking iNOS. Conclusions. iNOS is required in the process of cutaneous wound healing. Lacking iNOS retards macrophage invasion and its expression of fibrogenic components that might further impair fibrogenic behaviors of fibroblasts.http://dx.doi.org/10.1155/2017/2184040 |
| spellingShingle | Takashi Kitano Hiroshi Yamada Maki Kida Yuka Okada Shizuya Saika Munehito Yoshida Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse Dermatology Research and Practice |
| title | Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse |
| title_full | Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse |
| title_fullStr | Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse |
| title_full_unstemmed | Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse |
| title_short | Impaired Healing of a Cutaneous Wound in an Inducible Nitric Oxide Synthase-Knockout Mouse |
| title_sort | impaired healing of a cutaneous wound in an inducible nitric oxide synthase knockout mouse |
| url | http://dx.doi.org/10.1155/2017/2184040 |
| work_keys_str_mv | AT takashikitano impairedhealingofacutaneouswoundinaninduciblenitricoxidesynthaseknockoutmouse AT hiroshiyamada impairedhealingofacutaneouswoundinaninduciblenitricoxidesynthaseknockoutmouse AT makikida impairedhealingofacutaneouswoundinaninduciblenitricoxidesynthaseknockoutmouse AT yukaokada impairedhealingofacutaneouswoundinaninduciblenitricoxidesynthaseknockoutmouse AT shizuyasaika impairedhealingofacutaneouswoundinaninduciblenitricoxidesynthaseknockoutmouse AT munehitoyoshida impairedhealingofacutaneouswoundinaninduciblenitricoxidesynthaseknockoutmouse |