Anti-laminin-1 Autoantibodies, Pregnancy Loss and Endometriosis
Laminin-1 is a major component and multifunctional glycoprotein of basement membranes that consists of three different subunits, α1, β1 and γ1 chains. It is the earliest synthesized network-forming protein during embryogenesis and plays an important role in embryo...
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| Format: | Article |
| Language: | English |
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Wiley
2004-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520400001678 |
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| author | Junko Inagaki Akane Kondo Luis R. Lopez Yehuda Shoenfeld Eiji Matsuura |
| author_facet | Junko Inagaki Akane Kondo Luis R. Lopez Yehuda Shoenfeld Eiji Matsuura |
| author_sort | Junko Inagaki |
| collection | DOAJ |
| description | Laminin-1 is a major component and multifunctional glycoprotein of basement
membranes that consists of three different subunits, α1, β1 and γ1 chains. It is the
earliest synthesized network-forming protein during embryogenesis and plays
an important role in embryonic development, embryonic implantation and
placentation. We have recently shown that IgG anti-laminin-1 antibodies were
significantly associated with recurrent first-trimester miscarriages and with
subsequent pregnancy outcome. Interestingly, these antibodies were also observed
in patients with endometriosis-associated infertility but not in patients with other
causes of infertility, including tubal factors, hormonal and uterine abnormalities.
Laminin-α1, -β1 and -γ1 mRNAs have been detected in 90% of endometriotic lesions
and all laminin-α1, -β1 and -γ1 chains were localized in the basement membranes of
glandular epithelium in endometriotic peritoneal lesions. Western blot analysis
showed that anti-laminin-1 antibodies from those patients reacted with all
laminin-1's chains. ELISA also confirmed that one of the target epitopes for these
antibodies was located in a particular region of the laminin-1 molecule, i.e. the
carboxyl-terminal globular G domain of α1 chain. IgM monoclonal anti-laminin-1
autoantibody, that we recently established, also recognized the G domain.
Anti-laminin-1 antibodies from mice immunized with –mouse— laminin-1, caused
a higher fetal resorption rate with lower embryonic and placental weights. Thus,
anti-laminin-1 antibodies may be important in development of autoimmune-mediated
reproductive failures and the
assessment of the antibodies may provide a novel non-invasive diagnosis of
endometriosis. |
| format | Article |
| id | doaj-art-054bb4c1f8ec4e47869718f639160250 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-054bb4c1f8ec4e47869718f6391602502025-02-03T07:25:57ZengWileyClinical and Developmental Immunology1740-25221740-25302004-01-01113-426126610.1080/17402520400001678Anti-laminin-1 Autoantibodies, Pregnancy Loss and EndometriosisJunko Inagaki0Akane Kondo1Luis R. Lopez2Yehuda Shoenfeld3Eiji Matsuura4Department of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry, Okayama, JapanDepartment of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry, Okayama, JapanCorgenix Inc., Westminster, CO, USADepartment of Medicine B and Center for Autoimmune Diseases, Sheba Medical Center, hashomer, IsraelDepartment of Cell Chemistry, Okayama University Graduate School of Medicine and Dentistry, Okayama, JapanLaminin-1 is a major component and multifunctional glycoprotein of basement membranes that consists of three different subunits, α1, β1 and γ1 chains. It is the earliest synthesized network-forming protein during embryogenesis and plays an important role in embryonic development, embryonic implantation and placentation. We have recently shown that IgG anti-laminin-1 antibodies were significantly associated with recurrent first-trimester miscarriages and with subsequent pregnancy outcome. Interestingly, these antibodies were also observed in patients with endometriosis-associated infertility but not in patients with other causes of infertility, including tubal factors, hormonal and uterine abnormalities. Laminin-α1, -β1 and -γ1 mRNAs have been detected in 90% of endometriotic lesions and all laminin-α1, -β1 and -γ1 chains were localized in the basement membranes of glandular epithelium in endometriotic peritoneal lesions. Western blot analysis showed that anti-laminin-1 antibodies from those patients reacted with all laminin-1's chains. ELISA also confirmed that one of the target epitopes for these antibodies was located in a particular region of the laminin-1 molecule, i.e. the carboxyl-terminal globular G domain of α1 chain. IgM monoclonal anti-laminin-1 autoantibody, that we recently established, also recognized the G domain. Anti-laminin-1 antibodies from mice immunized with –mouse— laminin-1, caused a higher fetal resorption rate with lower embryonic and placental weights. Thus, anti-laminin-1 antibodies may be important in development of autoimmune-mediated reproductive failures and the assessment of the antibodies may provide a novel non-invasive diagnosis of endometriosis.http://dx.doi.org/10.1080/17402520400001678 |
| spellingShingle | Junko Inagaki Akane Kondo Luis R. Lopez Yehuda Shoenfeld Eiji Matsuura Anti-laminin-1 Autoantibodies, Pregnancy Loss and Endometriosis Clinical and Developmental Immunology |
| title | Anti-laminin-1 Autoantibodies, Pregnancy Loss and Endometriosis |
| title_full | Anti-laminin-1 Autoantibodies, Pregnancy Loss and Endometriosis |
| title_fullStr | Anti-laminin-1 Autoantibodies, Pregnancy Loss and Endometriosis |
| title_full_unstemmed | Anti-laminin-1 Autoantibodies, Pregnancy Loss and Endometriosis |
| title_short | Anti-laminin-1 Autoantibodies, Pregnancy Loss and Endometriosis |
| title_sort | anti laminin 1 autoantibodies pregnancy loss and endometriosis |
| url | http://dx.doi.org/10.1080/17402520400001678 |
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