Anti-laminin-1 Autoantibodies, Pregnancy Loss and Endometriosis
Laminin-1 is a major component and multifunctional glycoprotein of basement membranes that consists of three different subunits, α1, β1 and γ1 chains. It is the earliest synthesized network-forming protein during embryogenesis and plays an important role in embryo...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2004-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520400001678 |
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| Summary: | Laminin-1 is a major component and multifunctional glycoprotein of basement
membranes that consists of three different subunits, α1, β1 and γ1 chains. It is the
earliest synthesized network-forming protein during embryogenesis and plays
an important role in embryonic development, embryonic implantation and
placentation. We have recently shown that IgG anti-laminin-1 antibodies were
significantly associated with recurrent first-trimester miscarriages and with
subsequent pregnancy outcome. Interestingly, these antibodies were also observed
in patients with endometriosis-associated infertility but not in patients with other
causes of infertility, including tubal factors, hormonal and uterine abnormalities.
Laminin-α1, -β1 and -γ1 mRNAs have been detected in 90% of endometriotic lesions
and all laminin-α1, -β1 and -γ1 chains were localized in the basement membranes of
glandular epithelium in endometriotic peritoneal lesions. Western blot analysis
showed that anti-laminin-1 antibodies from those patients reacted with all
laminin-1's chains. ELISA also confirmed that one of the target epitopes for these
antibodies was located in a particular region of the laminin-1 molecule, i.e. the
carboxyl-terminal globular G domain of α1 chain. IgM monoclonal anti-laminin-1
autoantibody, that we recently established, also recognized the G domain.
Anti-laminin-1 antibodies from mice immunized with –mouse— laminin-1, caused
a higher fetal resorption rate with lower embryonic and placental weights. Thus,
anti-laminin-1 antibodies may be important in development of autoimmune-mediated
reproductive failures and the
assessment of the antibodies may provide a novel non-invasive diagnosis of
endometriosis. |
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| ISSN: | 1740-2522 1740-2530 |