Proliferation and gene expression data of rabbit Achilles tenocyte in vitro culture in the presence of high-molecular weight hyaluronic acid (1.01–1.8 MDa)Mendeley DataMendeley Data

Hyaluronic acid (HA) is a natural bio macromolecule and a good bio lubricant. As it is a predominant component of the synovial fluid, the aim was to explore the effects HA on rabbit Achilles tenocytes in vitro. The data set presents proliferation assessed by the Alamar Blue® assay; and gene expressi...

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Main Authors: Iris Miescher, Petra Wolint, Christine Opelz, Jess G. Snedeker, Pietro Giovanoli, Maurizio Calcagni, Johanna Buschmann
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Data in Brief
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Online Access:http://www.sciencedirect.com/science/article/pii/S235234092500191X
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Summary:Hyaluronic acid (HA) is a natural bio macromolecule and a good bio lubricant. As it is a predominant component of the synovial fluid, the aim was to explore the effects HA on rabbit Achilles tenocytes in vitro. The data set presents proliferation assessed by the Alamar Blue® assay; and gene expression of tenocytes that were cultured either with or without supplementation of high-molecular weight HA in a concentration of 1.4 mg/mL. There was a daily medium exchange with fresh HA supplementation. On days 3, 7 and 14 the following target genes were analysed and manifold induction was calculated against the control (no HA): Biglycan, Decorin, Aggrecan, Collagen 1A1, Collagen 1A2, Collagen 3, LOX, IL-6, TNF-α, PAR-2, ALOX-15, Tenascin-C, Tenomodulin, Mohawk, α-SMA, TIMP-1, MMP-2, MMP-9, and ki67. These data might be interesting for researchers working in the tendon field.
ISSN:2352-3409