A cross-study analysis of the effect of a dual-strain probiotic applied via the waterline on the growth performance and gut health of broilers under a mild necrotic enteritis challenge
Probiotics offer potential as an approach for the prevention and control of poultry intestinal diseases, but external factors can influence the birds’ response. Combining data from multiple trials provides greater confidence around efficacy under varying production conditions. Therefore, this study...
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011283 |
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| author | S.A.S. van der Klein M. Bernardeau Q. Wang K. Gibbs |
| author_facet | S.A.S. van der Klein M. Bernardeau Q. Wang K. Gibbs |
| author_sort | S.A.S. van der Klein |
| collection | DOAJ |
| description | Probiotics offer potential as an approach for the prevention and control of poultry intestinal diseases, but external factors can influence the birds’ response. Combining data from multiple trials provides greater confidence around efficacy under varying production conditions. Therefore, this study combined data from three separate trials analyzing the effect of a dual-strain probiotic comprising Lactobacillus acidophilus AG01 and Bifidobacterium animalis subspecies lactis AG02 on broilers during a mild necrotic enteritis (NE) challenge. In each, 1,440 broilers were assigned to floor-pens (40 birds/pen, 12 pens/treatment) in a completely randomized design. Treatments in each trial were a non-challenged control (C); challenged control (10 x dose of Eimeria/bird on d 14 and 1.0 x ∼109 colony forming units (CFU)/bird of C. perfringens on d 16-20; CC); and CC supplemented daily via the waterline with 1 × 109 CFU/bird of probiotic (CC+Probiotic). Birds were fed corn-soybean mealbased diets by phase (starter: 0 to 14, grower: 15 to 28, finisher: 29 to 42 d of age) ad libitum. Growth performance was monitored over 42 d, NE lesion scoring performed on d 21 and 28 in all trials, and, in Trial 3 only, cecal microbiota composition was analyzed on d 28. From d 1 to 42, CC birds exhibited reduced BW, BW gain (BWG), and feed intake (FI) (-9.2 %, -9.5 %, -5.0 %, respectively; P < 0.05), increased FCR and mortality (+8.5 points and 1.3 % points, respectively; P < 0.05) compared to C, and increased NE induction on d 28 (67.8 vs. 9.4 %, P <0.05). Compared to CC, CC+Probiotic birds exhibited increased BW, BWG and FI (d 42: +6.9 %, +7.1 %, +4.0 %; P < 0.05) and reduced FCR, mortality and d 28 NE lesion scores (-0.5 points, -1.4 % points and -57.1 %, respectively; P < 0.05). The composition of the cecal microbiota of CC+Probiotic birds at 28 d of age exhibited higher abundance of butyrogenic bacterial genera in Trial 3, which may have contributed to the beneficial effects of the probiotic. The results demonstrate that the probiotic ameliorated the negative effects of a mild NE-challenge on growth performance and intestinal symptoms over three trials incorporating variation in season and bird breed. |
| format | Article |
| id | doaj-art-05183096efd94ffb9da19d887ed42571 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Poultry Science |
| spelling | doaj-art-05183096efd94ffb9da19d887ed425712025-01-22T05:40:26ZengElsevierPoultry Science0032-57912025-01-011041104550A cross-study analysis of the effect of a dual-strain probiotic applied via the waterline on the growth performance and gut health of broilers under a mild necrotic enteritis challengeS.A.S. van der Klein0M. Bernardeau1Q. Wang2K. Gibbs3Danisco Animal Nutrition & Health, IFF, Willem Einthovenstraat 4 2342 BG, Oegstgeest, The Netherlands; Corresponding authorNormandy University, UNICAEN, ABTE 14000, Caen, FranceHealth & Biosciences, IFF, Wilmington, Delaware, USADanisco Animal Nutrition & Health, IFF, Willem Einthovenstraat 4 2342 BG, Oegstgeest, The NetherlandsProbiotics offer potential as an approach for the prevention and control of poultry intestinal diseases, but external factors can influence the birds’ response. Combining data from multiple trials provides greater confidence around efficacy under varying production conditions. Therefore, this study combined data from three separate trials analyzing the effect of a dual-strain probiotic comprising Lactobacillus acidophilus AG01 and Bifidobacterium animalis subspecies lactis AG02 on broilers during a mild necrotic enteritis (NE) challenge. In each, 1,440 broilers were assigned to floor-pens (40 birds/pen, 12 pens/treatment) in a completely randomized design. Treatments in each trial were a non-challenged control (C); challenged control (10 x dose of Eimeria/bird on d 14 and 1.0 x ∼109 colony forming units (CFU)/bird of C. perfringens on d 16-20; CC); and CC supplemented daily via the waterline with 1 × 109 CFU/bird of probiotic (CC+Probiotic). Birds were fed corn-soybean mealbased diets by phase (starter: 0 to 14, grower: 15 to 28, finisher: 29 to 42 d of age) ad libitum. Growth performance was monitored over 42 d, NE lesion scoring performed on d 21 and 28 in all trials, and, in Trial 3 only, cecal microbiota composition was analyzed on d 28. From d 1 to 42, CC birds exhibited reduced BW, BW gain (BWG), and feed intake (FI) (-9.2 %, -9.5 %, -5.0 %, respectively; P < 0.05), increased FCR and mortality (+8.5 points and 1.3 % points, respectively; P < 0.05) compared to C, and increased NE induction on d 28 (67.8 vs. 9.4 %, P <0.05). Compared to CC, CC+Probiotic birds exhibited increased BW, BWG and FI (d 42: +6.9 %, +7.1 %, +4.0 %; P < 0.05) and reduced FCR, mortality and d 28 NE lesion scores (-0.5 points, -1.4 % points and -57.1 %, respectively; P < 0.05). The composition of the cecal microbiota of CC+Probiotic birds at 28 d of age exhibited higher abundance of butyrogenic bacterial genera in Trial 3, which may have contributed to the beneficial effects of the probiotic. The results demonstrate that the probiotic ameliorated the negative effects of a mild NE-challenge on growth performance and intestinal symptoms over three trials incorporating variation in season and bird breed.http://www.sciencedirect.com/science/article/pii/S0032579124011283BroilerCoccidiosisMicrobiomeNecrotic enteritisProbiotic |
| spellingShingle | S.A.S. van der Klein M. Bernardeau Q. Wang K. Gibbs A cross-study analysis of the effect of a dual-strain probiotic applied via the waterline on the growth performance and gut health of broilers under a mild necrotic enteritis challenge Poultry Science Broiler Coccidiosis Microbiome Necrotic enteritis Probiotic |
| title | A cross-study analysis of the effect of a dual-strain probiotic applied via the waterline on the growth performance and gut health of broilers under a mild necrotic enteritis challenge |
| title_full | A cross-study analysis of the effect of a dual-strain probiotic applied via the waterline on the growth performance and gut health of broilers under a mild necrotic enteritis challenge |
| title_fullStr | A cross-study analysis of the effect of a dual-strain probiotic applied via the waterline on the growth performance and gut health of broilers under a mild necrotic enteritis challenge |
| title_full_unstemmed | A cross-study analysis of the effect of a dual-strain probiotic applied via the waterline on the growth performance and gut health of broilers under a mild necrotic enteritis challenge |
| title_short | A cross-study analysis of the effect of a dual-strain probiotic applied via the waterline on the growth performance and gut health of broilers under a mild necrotic enteritis challenge |
| title_sort | cross study analysis of the effect of a dual strain probiotic applied via the waterline on the growth performance and gut health of broilers under a mild necrotic enteritis challenge |
| topic | Broiler Coccidiosis Microbiome Necrotic enteritis Probiotic |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011283 |
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