Optimization of Protease and Amylase Production by Rhizopus oryzae Cultivated on Bread Waste Using Solid-State Fermentation
This research was carried for the coproduction of two industrial enzymes: α-amylase and protease via SSF by Rhizopus oryzae on humidified bread waste. Fermentation time, inoculum size, initial moisture content, salt solutions, and the thickness of the substrate were investigated one by one. Fungus c...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/3738181 |
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| author | Olfa Benabda Sana M’hir Mariam Kasmi Wissem Mnif Moktar Hamdi |
| author_facet | Olfa Benabda Sana M’hir Mariam Kasmi Wissem Mnif Moktar Hamdi |
| author_sort | Olfa Benabda |
| collection | DOAJ |
| description | This research was carried for the coproduction of two industrial enzymes: α-amylase and protease via SSF by Rhizopus oryzae on humidified bread waste. Fermentation time, inoculum size, initial moisture content, salt solutions, and the thickness of the substrate were investigated one by one. Fungus culture was carried out in sterile aluminum trays, and pH was adjusted to 5.5. The main results showed that the highest levels of enzyme production were obtained at 120 h, 65% relative humidity, height media of 1 cm, 105 spore/g, and M-9 solution (g/L): NaH2PO4, 12.8; KH2PO4, 3; NaCl, 0.5; NH4Cl, 1; MgSO4 7H2O, 0.5; CaCl2 2H2O, 0.01. α-Amylase (100 U/g) and protease (2400 U/g) produced by SSF from Rhizopus oryzae (CH4) on BW as substrate are of great interest in industries and could be valorized as enhancers of the bread making process. |
| format | Article |
| id | doaj-art-0510593197224c2cbcc4ef64ccd27b7e |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-0510593197224c2cbcc4ef64ccd27b7e2025-02-03T01:11:44ZengWileyJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/37381813738181Optimization of Protease and Amylase Production by Rhizopus oryzae Cultivated on Bread Waste Using Solid-State FermentationOlfa Benabda0Sana M’hir1Mariam Kasmi2Wissem Mnif3Moktar Hamdi4Laboratoire d’Ecologie et de Technologie Microbienne (LETMi), Institut National des Sciences Appliquées et de Technologie (INSAT), 676 Centre Urbain Nord BP, University of Carthage, Tunis Cedex 1080, TunisiaLaboratoire d’Ecologie et de Technologie Microbienne (LETMi), Institut National des Sciences Appliquées et de Technologie (INSAT), 676 Centre Urbain Nord BP, University of Carthage, Tunis Cedex 1080, TunisiaHigh School of Food Industry of Tunis (ESIAT), University of Carthage, Tunis, TunisiaUniversity of Manouba, ISBST, BVBGR-LR11ES31, Biotechpole Sidi Thabet, 2020 Ariana, TunisiaLaboratoire d’Ecologie et de Technologie Microbienne (LETMi), Institut National des Sciences Appliquées et de Technologie (INSAT), 676 Centre Urbain Nord BP, University of Carthage, Tunis Cedex 1080, TunisiaThis research was carried for the coproduction of two industrial enzymes: α-amylase and protease via SSF by Rhizopus oryzae on humidified bread waste. Fermentation time, inoculum size, initial moisture content, salt solutions, and the thickness of the substrate were investigated one by one. Fungus culture was carried out in sterile aluminum trays, and pH was adjusted to 5.5. The main results showed that the highest levels of enzyme production were obtained at 120 h, 65% relative humidity, height media of 1 cm, 105 spore/g, and M-9 solution (g/L): NaH2PO4, 12.8; KH2PO4, 3; NaCl, 0.5; NH4Cl, 1; MgSO4 7H2O, 0.5; CaCl2 2H2O, 0.01. α-Amylase (100 U/g) and protease (2400 U/g) produced by SSF from Rhizopus oryzae (CH4) on BW as substrate are of great interest in industries and could be valorized as enhancers of the bread making process.http://dx.doi.org/10.1155/2019/3738181 |
| spellingShingle | Olfa Benabda Sana M’hir Mariam Kasmi Wissem Mnif Moktar Hamdi Optimization of Protease and Amylase Production by Rhizopus oryzae Cultivated on Bread Waste Using Solid-State Fermentation Journal of Chemistry |
| title | Optimization of Protease and Amylase Production by Rhizopus oryzae Cultivated on Bread Waste Using Solid-State Fermentation |
| title_full | Optimization of Protease and Amylase Production by Rhizopus oryzae Cultivated on Bread Waste Using Solid-State Fermentation |
| title_fullStr | Optimization of Protease and Amylase Production by Rhizopus oryzae Cultivated on Bread Waste Using Solid-State Fermentation |
| title_full_unstemmed | Optimization of Protease and Amylase Production by Rhizopus oryzae Cultivated on Bread Waste Using Solid-State Fermentation |
| title_short | Optimization of Protease and Amylase Production by Rhizopus oryzae Cultivated on Bread Waste Using Solid-State Fermentation |
| title_sort | optimization of protease and amylase production by rhizopus oryzae cultivated on bread waste using solid state fermentation |
| url | http://dx.doi.org/10.1155/2019/3738181 |
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