Expression of IL-18, IL-18 Binding Protein, and IL-18 Receptor by Normal and Cancerous Human Ovarian Tissues: Possible Implication of IL-18 in the Pathogenesis of Ovarian Carcinoma
Proinflammatory cytokine IL-18 has been shown to be elevated in the sera of ovarian carcinoma patients. The aim of the study was to examine the levels and cellular origin of IL-18, IL-18 binding protein, and IL-18 receptor in normal and cancerous ovarian tissues. Ovarian tissue samples were examined...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/914954 |
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| author | Liat Medina Alex Rabinovich Benjamin Piura Victor Dyomin Ruthy Shaco Levy Mahmoud Huleihel |
| author_facet | Liat Medina Alex Rabinovich Benjamin Piura Victor Dyomin Ruthy Shaco Levy Mahmoud Huleihel |
| author_sort | Liat Medina |
| collection | DOAJ |
| description | Proinflammatory cytokine IL-18 has been shown to be elevated in the sera of ovarian carcinoma patients. The aim of the study was to examine the levels and cellular origin of IL-18, IL-18 binding protein, and IL-18 receptor in normal and cancerous ovarian tissues. Ovarian tissue samples were examined by immunohistochemical staining for IL-18, IL-18BP, and IL-18R and mRNA of these cytokines was analyzed with semiquantitative PT-PCR. IL-18 levels were significantly higher in cancerous ovarian tissues (P=0.0007), IL-18BP levels were significantly higher in normal ovarian tissues (P=0.04), and the ratio of IL-18/IL-18BP was significantly higher in cancerous ovarian tissues (P=0.036). Cancerous ovarian tissues expressed significantly higher IL-18 mRNA levels (P=0.025), while there was no difference in the expression of IL-18BP mRNA and IL-18R mRNA between cancerous and normal ovarian tissues. IL-18 and IL-18BP were expressed dominantly in the epithelial cells of both cancerous and normal ovarian tissues, while IL-18R was expressed dominantly in the epithelial cells of cancerous ovarian tissues but expressed similarly in the epithelial and stromal cells of normal cancerous tissues. This study indicates a possible role of IL-18, IL-18BP, and IL-18R in the pathogenesis of epithelial ovarian carcinoma. |
| format | Article |
| id | doaj-art-04dcc329718f41bbb872d1acf2027248 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-04dcc329718f41bbb872d1acf20272482025-02-03T07:25:42ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/914954914954Expression of IL-18, IL-18 Binding Protein, and IL-18 Receptor by Normal and Cancerous Human Ovarian Tissues: Possible Implication of IL-18 in the Pathogenesis of Ovarian CarcinomaLiat Medina0Alex Rabinovich1Benjamin Piura2Victor Dyomin3Ruthy Shaco Levy4Mahmoud Huleihel5The Shraga Segal Department of Microbiology, Immunology and Genetics, Be’er Sheva, IsraelFaculty of Health Sciences, Ben-Gurion University of the Negev, 84105 Be’er Sheva, IsraelFaculty of Health Sciences, Ben-Gurion University of the Negev, 84105 Be’er Sheva, IsraelUnit of Gynecologic Oncology, Division of Obstetrics and Gynecology, Soroka University Medical Center, 84105 Be’er Sheva, IsraelUnit of Gynecologic Oncology, Division of Obstetrics and Gynecology, Soroka University Medical Center, 84105 Be’er Sheva, IsraelThe Shraga Segal Department of Microbiology, Immunology and Genetics, Be’er Sheva, IsraelProinflammatory cytokine IL-18 has been shown to be elevated in the sera of ovarian carcinoma patients. The aim of the study was to examine the levels and cellular origin of IL-18, IL-18 binding protein, and IL-18 receptor in normal and cancerous ovarian tissues. Ovarian tissue samples were examined by immunohistochemical staining for IL-18, IL-18BP, and IL-18R and mRNA of these cytokines was analyzed with semiquantitative PT-PCR. IL-18 levels were significantly higher in cancerous ovarian tissues (P=0.0007), IL-18BP levels were significantly higher in normal ovarian tissues (P=0.04), and the ratio of IL-18/IL-18BP was significantly higher in cancerous ovarian tissues (P=0.036). Cancerous ovarian tissues expressed significantly higher IL-18 mRNA levels (P=0.025), while there was no difference in the expression of IL-18BP mRNA and IL-18R mRNA between cancerous and normal ovarian tissues. IL-18 and IL-18BP were expressed dominantly in the epithelial cells of both cancerous and normal ovarian tissues, while IL-18R was expressed dominantly in the epithelial cells of cancerous ovarian tissues but expressed similarly in the epithelial and stromal cells of normal cancerous tissues. This study indicates a possible role of IL-18, IL-18BP, and IL-18R in the pathogenesis of epithelial ovarian carcinoma.http://dx.doi.org/10.1155/2014/914954 |
| spellingShingle | Liat Medina Alex Rabinovich Benjamin Piura Victor Dyomin Ruthy Shaco Levy Mahmoud Huleihel Expression of IL-18, IL-18 Binding Protein, and IL-18 Receptor by Normal and Cancerous Human Ovarian Tissues: Possible Implication of IL-18 in the Pathogenesis of Ovarian Carcinoma Mediators of Inflammation |
| title | Expression of IL-18, IL-18 Binding Protein, and IL-18 Receptor by Normal and Cancerous Human Ovarian Tissues: Possible Implication of IL-18 in the Pathogenesis of Ovarian Carcinoma |
| title_full | Expression of IL-18, IL-18 Binding Protein, and IL-18 Receptor by Normal and Cancerous Human Ovarian Tissues: Possible Implication of IL-18 in the Pathogenesis of Ovarian Carcinoma |
| title_fullStr | Expression of IL-18, IL-18 Binding Protein, and IL-18 Receptor by Normal and Cancerous Human Ovarian Tissues: Possible Implication of IL-18 in the Pathogenesis of Ovarian Carcinoma |
| title_full_unstemmed | Expression of IL-18, IL-18 Binding Protein, and IL-18 Receptor by Normal and Cancerous Human Ovarian Tissues: Possible Implication of IL-18 in the Pathogenesis of Ovarian Carcinoma |
| title_short | Expression of IL-18, IL-18 Binding Protein, and IL-18 Receptor by Normal and Cancerous Human Ovarian Tissues: Possible Implication of IL-18 in the Pathogenesis of Ovarian Carcinoma |
| title_sort | expression of il 18 il 18 binding protein and il 18 receptor by normal and cancerous human ovarian tissues possible implication of il 18 in the pathogenesis of ovarian carcinoma |
| url | http://dx.doi.org/10.1155/2014/914954 |
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