Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications
Purpose. To assess FGF-β, TGF-β, and COX2 expression and immunocompetent cells in the orbital tissue of patients with severe and mild Graves’ orbitopathy. Patients and Methods. Orbital tissue was taken from 27 patients with GO: (1) severe GO (n=18), the mean clinical activity score (CAS) being 8.5 (...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/412158 |
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| author | Przemyslaw Pawlowski Joanna Reszec Anja Eckstein Kristian Johnson Andrzej Grzybowski Lech Chyczewski Janusz Mysliwiec |
| author_facet | Przemyslaw Pawlowski Joanna Reszec Anja Eckstein Kristian Johnson Andrzej Grzybowski Lech Chyczewski Janusz Mysliwiec |
| author_sort | Przemyslaw Pawlowski |
| collection | DOAJ |
| description | Purpose. To assess FGF-β, TGF-β, and COX2 expression and immunocompetent cells in the orbital tissue of patients with severe and mild Graves’ orbitopathy. Patients and Methods. Orbital tissue was taken from 27 patients with GO: (1) severe GO (n=18), the mean clinical activity score (CAS) being 8.5 (SD 2.5); and (2) mild GO (n=9), the mean CAS being 2.2 (SD 0.8), and from 10 individuals undergoing blepharoplasty. The expression of CD4+, CD8+, CD20+, and CD68 and FGF-β, TGF-β, and COX2 in the orbital tissue was evaluated by immunohistochemical methods. Results. We demonstrated predominant CD4+ T cells in severe GO. CD68 expression was observed in the fibrous connective area of mild GO and was robust in severe GO, while the prominent TGF-β expression was seen in all GO. Increased FGF-β expression was observed in the fibroblasts and adipocytes of severe GO. No expression of COX2 was found in patients with GO. Conclusions. Macrophages and CD4 T lymphocytes are both engaged in the active/severe and long stage of inflammation in the orbital tissue. FGF-β and TGF-β expression may contribute to tissue remodeling, fibrosis, and perpetuation of inflammation in the orbital tissue of GO especially in severe GO. |
| format | Article |
| id | doaj-art-04d73b08cdfe4d7191b5b1690e509d6f |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-04d73b08cdfe4d7191b5b1690e509d6f2025-02-03T07:25:42ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/412158412158Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical ImplicationsPrzemyslaw Pawlowski0Joanna Reszec1Anja Eckstein2Kristian Johnson3Andrzej Grzybowski4Lech Chyczewski5Janusz Mysliwiec6Department of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Białystok, 13 Waszyngtona Street, 15-269 Białystok, PolandDepartment of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Białystok, 13 Waszyngtona Street, 15-269 Białystok, PolandDepartment of Ophthalmology, University Hospital Essen and University of Duisburg-Essen, Hufeland Straße 55, 45-122 Essen, GermanyBayer Healtcare, Kaiser-Wilhelm-Allee 10, Leverkusen, 51-373 Nordshein-Westfalen, GermanyDepartment of Ophthalmology, Poznań City Hospital, 3 Szwajcarska Street, 61-285 Poznań, PolandDepartment of Medical Pathomorphology, Cathedral of Biostructure, Medical University of Białystok, 13 Waszyngtona Street, 15-269 Białystok, PolandDepartment of Nuclear Medicine, Medical University of Białystok, 24A Skłodowskiej-Curie Street, 15-276 Białystok, PolandPurpose. To assess FGF-β, TGF-β, and COX2 expression and immunocompetent cells in the orbital tissue of patients with severe and mild Graves’ orbitopathy. Patients and Methods. Orbital tissue was taken from 27 patients with GO: (1) severe GO (n=18), the mean clinical activity score (CAS) being 8.5 (SD 2.5); and (2) mild GO (n=9), the mean CAS being 2.2 (SD 0.8), and from 10 individuals undergoing blepharoplasty. The expression of CD4+, CD8+, CD20+, and CD68 and FGF-β, TGF-β, and COX2 in the orbital tissue was evaluated by immunohistochemical methods. Results. We demonstrated predominant CD4+ T cells in severe GO. CD68 expression was observed in the fibrous connective area of mild GO and was robust in severe GO, while the prominent TGF-β expression was seen in all GO. Increased FGF-β expression was observed in the fibroblasts and adipocytes of severe GO. No expression of COX2 was found in patients with GO. Conclusions. Macrophages and CD4 T lymphocytes are both engaged in the active/severe and long stage of inflammation in the orbital tissue. FGF-β and TGF-β expression may contribute to tissue remodeling, fibrosis, and perpetuation of inflammation in the orbital tissue of GO especially in severe GO.http://dx.doi.org/10.1155/2014/412158 |
| spellingShingle | Przemyslaw Pawlowski Joanna Reszec Anja Eckstein Kristian Johnson Andrzej Grzybowski Lech Chyczewski Janusz Mysliwiec Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications Mediators of Inflammation |
| title | Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications |
| title_full | Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications |
| title_fullStr | Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications |
| title_full_unstemmed | Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications |
| title_short | Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications |
| title_sort | markers of inflammation and fibrosis in the orbital fat connective tissue of patients with graves orbitopathy clinical implications |
| url | http://dx.doi.org/10.1155/2014/412158 |
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