Systematic Characterization of the Chemical Components of Canna edulis Ker-Gawl Based on UHPLC Q-Exactive Orbitrap MS Technology
Canna edulis Ker-Gawl is a versatile crop that integrates food, energy, and feed in China. The rhizome is a traditional Chinese medicine with a long history. However, there are few studies on chemical components. Crude plant extracts are a complex mixture of several biologically active secondary met...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2023/8230958 |
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| author | Liqiao Lv Chi Zhang Jiahui Wu Dachuan Zhang Fangyuan Zhao Lv Bu Aga Nan Wang Junling Li Leimengyuan Tang Baosheng Zhao Chunguo Wang Xueyong Wang |
| author_facet | Liqiao Lv Chi Zhang Jiahui Wu Dachuan Zhang Fangyuan Zhao Lv Bu Aga Nan Wang Junling Li Leimengyuan Tang Baosheng Zhao Chunguo Wang Xueyong Wang |
| author_sort | Liqiao Lv |
| collection | DOAJ |
| description | Canna edulis Ker-Gawl is a versatile crop that integrates food, energy, and feed in China. The rhizome is a traditional Chinese medicine with a long history. However, there are few studies on chemical components. Crude plant extracts are a complex mixture of several biologically active secondary metabolites. Therefore, rapid and accurate identification and quantification are critical in phytochemical analysis. An efficient approach based on ultrahigh-performance liquid chromatography coupled with Q-Exactive Orbitrap tandem mass spectrometry (UHPLC Q-Exactive Orbitrap MS) was used to analyze the chemical components systematically. We used retention time, accurate molecular weight, parent peaks, fragment peaks, fragmentation characteristics, comparative analysis with the literature, reference standards, and standard databases, including ChemSpider, mzVault, MassBank, and mzCloud. A total of 54 chemical constituents were identified from the methanol extract of the rhizome, including 36 organic acids (with six phenolic acids), three phenols, one sugar (and its derivatives), one coumarin, one triterpenoid, ten N-containing organic compounds (including seven amino acids), and two other compounds. Moreover, three compounds were quantitatively analyzed and a methodological study of the three components was carried out. The established method provided satisfactory precision and accuracy, acceptable recovery, good linearity, and a reasonable detection limit. The UHPLC Q-Exactive Orbitrap MS method was used for the first time to accurately, quickly, and systematically characterize the compounds of the rhizome, revealing the pharmacodynamic substances and providing a theoretical basis for its quality control, in-depth research, and development. |
| format | Article |
| id | doaj-art-04cb194c415544ce95a5a40e2d018daf |
| institution | Kabale University |
| issn | 1745-4557 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-04cb194c415544ce95a5a40e2d018daf2025-02-03T06:45:10ZengWileyJournal of Food Quality1745-45572023-01-01202310.1155/2023/8230958Systematic Characterization of the Chemical Components of Canna edulis Ker-Gawl Based on UHPLC Q-Exactive Orbitrap MS TechnologyLiqiao Lv0Chi Zhang1Jiahui Wu2Dachuan Zhang3Fangyuan Zhao4Lv Bu Aga5Nan Wang6Junling Li7Leimengyuan Tang8Baosheng Zhao9Chunguo Wang10Xueyong Wang11School of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaBeijing Institute of Chinese MedicineBeijing Institute of Chinese MedicineSchool of Chinese Materia MedicaCanna edulis Ker-Gawl is a versatile crop that integrates food, energy, and feed in China. The rhizome is a traditional Chinese medicine with a long history. However, there are few studies on chemical components. Crude plant extracts are a complex mixture of several biologically active secondary metabolites. Therefore, rapid and accurate identification and quantification are critical in phytochemical analysis. An efficient approach based on ultrahigh-performance liquid chromatography coupled with Q-Exactive Orbitrap tandem mass spectrometry (UHPLC Q-Exactive Orbitrap MS) was used to analyze the chemical components systematically. We used retention time, accurate molecular weight, parent peaks, fragment peaks, fragmentation characteristics, comparative analysis with the literature, reference standards, and standard databases, including ChemSpider, mzVault, MassBank, and mzCloud. A total of 54 chemical constituents were identified from the methanol extract of the rhizome, including 36 organic acids (with six phenolic acids), three phenols, one sugar (and its derivatives), one coumarin, one triterpenoid, ten N-containing organic compounds (including seven amino acids), and two other compounds. Moreover, three compounds were quantitatively analyzed and a methodological study of the three components was carried out. The established method provided satisfactory precision and accuracy, acceptable recovery, good linearity, and a reasonable detection limit. The UHPLC Q-Exactive Orbitrap MS method was used for the first time to accurately, quickly, and systematically characterize the compounds of the rhizome, revealing the pharmacodynamic substances and providing a theoretical basis for its quality control, in-depth research, and development.http://dx.doi.org/10.1155/2023/8230958 |
| spellingShingle | Liqiao Lv Chi Zhang Jiahui Wu Dachuan Zhang Fangyuan Zhao Lv Bu Aga Nan Wang Junling Li Leimengyuan Tang Baosheng Zhao Chunguo Wang Xueyong Wang Systematic Characterization of the Chemical Components of Canna edulis Ker-Gawl Based on UHPLC Q-Exactive Orbitrap MS Technology Journal of Food Quality |
| title | Systematic Characterization of the Chemical Components of Canna edulis Ker-Gawl Based on UHPLC Q-Exactive Orbitrap MS Technology |
| title_full | Systematic Characterization of the Chemical Components of Canna edulis Ker-Gawl Based on UHPLC Q-Exactive Orbitrap MS Technology |
| title_fullStr | Systematic Characterization of the Chemical Components of Canna edulis Ker-Gawl Based on UHPLC Q-Exactive Orbitrap MS Technology |
| title_full_unstemmed | Systematic Characterization of the Chemical Components of Canna edulis Ker-Gawl Based on UHPLC Q-Exactive Orbitrap MS Technology |
| title_short | Systematic Characterization of the Chemical Components of Canna edulis Ker-Gawl Based on UHPLC Q-Exactive Orbitrap MS Technology |
| title_sort | systematic characterization of the chemical components of canna edulis ker gawl based on uhplc q exactive orbitrap ms technology |
| url | http://dx.doi.org/10.1155/2023/8230958 |
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