Expression of the BAX Gene, CO1 Gene, and their Relationship to the Motility and Spermatozoa Concentration of Rats Treated with Moringa oleifera Leaf Extract
Spermatogenesis is the process of spermatozoa formation. An increase in free radicals, such as in hyperglycemia conditions, causes oxidative stress, which can interfere with spermatogenesis. Oxidative stress is an inequality condition between the body’s capacity to produce antioxidants and the rise...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2025-03-01
|
| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/55446 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850150103751327744 |
|---|---|
| author | Uswatun Hasanah Cut Fauziah Nasihin saud Irsyad Andri Pramesyanti Pramono Dinda Hanifah Wahab Wafa Nabila Nawawi |
| author_facet | Uswatun Hasanah Cut Fauziah Nasihin saud Irsyad Andri Pramesyanti Pramono Dinda Hanifah Wahab Wafa Nabila Nawawi |
| author_sort | Uswatun Hasanah |
| collection | DOAJ |
| description |
Spermatogenesis is the process of spermatozoa formation. An increase in free radicals, such as in hyperglycemia conditions, causes oxidative stress, which can interfere with spermatogenesis. Oxidative stress is an inequality condition between the body’s capacity to produce antioxidants and the rise of free radicals. Antioxidants such as flavonoids are contained in plants such as Moringa oleifera leaves. During spermatogenesis, an apoptosis process involves the BAX gene. BAX is a gene that functions as pro-apoptosis. There is also a CO1 gene in mitochondria, which plays a role in cellular metabolism. Gene expression examination assesses the comparative threshold cycle (CT) with RT-PCR, and spermatozoa quality examination includes assessing spermatozoa concentration and motility. This study used 30 male white Sprague Dawley rats as the test animals, which were divided into five treatment groups: treatment of moringa leaf extract dose 200 mg/kg BW, treatment dose 300 mg/kg BW, treatment dose 400 mg/kg BW, normal control group, and positive control group. The results showed a decrease in BAX gene expression and CO1 gene expression in the treatment group of test animals. The results of sperm analysis showed an increase in spermatozoa concentration in all treatment groups compared to the control group. For sperm motility in the 400 mg/KgBB dose treatment, an increase in sperm motility compared to the control group. This study concludes that administering moringa leaf extract reduced the expression of the Bax and CO1 genes. However, no significant changes were observed in sperm concentration and motility within the treatment group.
|
| format | Article |
| id | doaj-art-04c55e96f40d4a6cb5d7f84b9b13d3ed |
| institution | OA Journals |
| issn | 1978-3019 2086-4094 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj-art-04c55e96f40d4a6cb5d7f84b9b13d3ed2025-08-20T02:26:40ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942025-03-0132410.4308/hjb.32.4.1003-1009Expression of the BAX Gene, CO1 Gene, and their Relationship to the Motility and Spermatozoa Concentration of Rats Treated with Moringa oleifera Leaf ExtractUswatun Hasanah0Cut Fauziah1Nasihin saud Irsyad2Andri Pramesyanti Pramono3Dinda Hanifah Wahab4Wafa Nabila Nawawi5Department of Molecular Biology, Faculty of Medicine, UPN Veteran Jakarta, Jakarta Selatan 12450, Indonesia. Research and Innovation National Agency (BRIN), Jakarta, IndonesiaDepartment of Molecular Biology, Faculty of Medicine, UPN Veteran Jakarta, Jakarta Selatan 12450, IndonesiaDepartment of Molecular Biology, Faculty of Medicine, UPN Veteran Jakarta, Jakarta Selatan 12450, IndonesiaResearch and Innovation National Agency (BRIN), Jakarta, IndonesiaFaculty of Medicine, UPN Veteran Jakarta, Jakarta Selatan 12450, IndonesiaFaculty of Medicine, UPN Veteran Jakarta, Jakarta Selatan 12450, Indonesia Spermatogenesis is the process of spermatozoa formation. An increase in free radicals, such as in hyperglycemia conditions, causes oxidative stress, which can interfere with spermatogenesis. Oxidative stress is an inequality condition between the body’s capacity to produce antioxidants and the rise of free radicals. Antioxidants such as flavonoids are contained in plants such as Moringa oleifera leaves. During spermatogenesis, an apoptosis process involves the BAX gene. BAX is a gene that functions as pro-apoptosis. There is also a CO1 gene in mitochondria, which plays a role in cellular metabolism. Gene expression examination assesses the comparative threshold cycle (CT) with RT-PCR, and spermatozoa quality examination includes assessing spermatozoa concentration and motility. This study used 30 male white Sprague Dawley rats as the test animals, which were divided into five treatment groups: treatment of moringa leaf extract dose 200 mg/kg BW, treatment dose 300 mg/kg BW, treatment dose 400 mg/kg BW, normal control group, and positive control group. The results showed a decrease in BAX gene expression and CO1 gene expression in the treatment group of test animals. The results of sperm analysis showed an increase in spermatozoa concentration in all treatment groups compared to the control group. For sperm motility in the 400 mg/KgBB dose treatment, an increase in sperm motility compared to the control group. This study concludes that administering moringa leaf extract reduced the expression of the Bax and CO1 genes. However, no significant changes were observed in sperm concentration and motility within the treatment group. https://journal.ipb.ac.id/index.php/hayati/article/view/55446 |
| spellingShingle | Uswatun Hasanah Cut Fauziah Nasihin saud Irsyad Andri Pramesyanti Pramono Dinda Hanifah Wahab Wafa Nabila Nawawi Expression of the BAX Gene, CO1 Gene, and their Relationship to the Motility and Spermatozoa Concentration of Rats Treated with Moringa oleifera Leaf Extract Hayati Journal of Biosciences |
| title | Expression of the BAX Gene, CO1 Gene, and their Relationship to the Motility and Spermatozoa Concentration of Rats Treated with Moringa oleifera Leaf Extract |
| title_full | Expression of the BAX Gene, CO1 Gene, and their Relationship to the Motility and Spermatozoa Concentration of Rats Treated with Moringa oleifera Leaf Extract |
| title_fullStr | Expression of the BAX Gene, CO1 Gene, and their Relationship to the Motility and Spermatozoa Concentration of Rats Treated with Moringa oleifera Leaf Extract |
| title_full_unstemmed | Expression of the BAX Gene, CO1 Gene, and their Relationship to the Motility and Spermatozoa Concentration of Rats Treated with Moringa oleifera Leaf Extract |
| title_short | Expression of the BAX Gene, CO1 Gene, and their Relationship to the Motility and Spermatozoa Concentration of Rats Treated with Moringa oleifera Leaf Extract |
| title_sort | expression of the bax gene co1 gene and their relationship to the motility and spermatozoa concentration of rats treated with moringa oleifera leaf extract |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/55446 |
| work_keys_str_mv | AT uswatunhasanah expressionofthebaxgeneco1geneandtheirrelationshiptothemotilityandspermatozoaconcentrationofratstreatedwithmoringaoleiferaleafextract AT cutfauziah expressionofthebaxgeneco1geneandtheirrelationshiptothemotilityandspermatozoaconcentrationofratstreatedwithmoringaoleiferaleafextract AT nasihinsaudirsyad expressionofthebaxgeneco1geneandtheirrelationshiptothemotilityandspermatozoaconcentrationofratstreatedwithmoringaoleiferaleafextract AT andripramesyantipramono expressionofthebaxgeneco1geneandtheirrelationshiptothemotilityandspermatozoaconcentrationofratstreatedwithmoringaoleiferaleafextract AT dindahanifahwahab expressionofthebaxgeneco1geneandtheirrelationshiptothemotilityandspermatozoaconcentrationofratstreatedwithmoringaoleiferaleafextract AT wafanabilanawawi expressionofthebaxgeneco1geneandtheirrelationshiptothemotilityandspermatozoaconcentrationofratstreatedwithmoringaoleiferaleafextract |