Multispectral Enhancement Method to Increase the Visual Differences of Tissue Structures in Stained Histopathology Images
In this paper we proposed a multispectral enhancement scheme in which the spectral colors of the stained tissue-structure of interest and its background can be independently modified by the user to further improve their visualization and color discrimination. The colors of the background objects are...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.3233/ACP-2012-0069 |
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| author | Pinky A. Bautista Yukako Yagi |
| author_facet | Pinky A. Bautista Yukako Yagi |
| author_sort | Pinky A. Bautista |
| collection | DOAJ |
| description | In this paper we proposed a multispectral enhancement scheme in which the spectral colors of the stained tissue-structure of interest and its background can be independently modified by the user to further improve their visualization and color discrimination. The colors of the background objects are modified by transforming their N-band spectra through an NxN transformation matrix, which is derived by mapping the representative samples of their original spectra to the spectra of their target colors using least mean square method. On the other hand, the color of the tissue structure of interest is modified by modulating the transformed spectra with the sum of the pixel’s spectral residual-errors at specific bands weighted through an NxN weighting matrix; the spectral error is derived by taking the difference between the pixel’s original spectrum and its reconstructed spectrum using the first M dominant principal component vectors in principal component analysis. Promising results were obtained on the visualization of the collagen fiber and the non-collagen tissue structures, e.g., nuclei, cytoplasm and red blood cells (RBC), in a hematoxylin and eosin (H&E) stained image. |
| format | Article |
| id | doaj-art-04c4c27ab66f43ed9a4b579629dd88dc |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-04c4c27ab66f43ed9a4b579629dd88dc2025-02-03T05:45:44ZengWileyAnalytical Cellular Pathology2210-71772210-71852012-01-01355-640742010.3233/ACP-2012-0069Multispectral Enhancement Method to Increase the Visual Differences of Tissue Structures in Stained Histopathology ImagesPinky A. Bautista0Yukako Yagi1Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USADepartment of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USAIn this paper we proposed a multispectral enhancement scheme in which the spectral colors of the stained tissue-structure of interest and its background can be independently modified by the user to further improve their visualization and color discrimination. The colors of the background objects are modified by transforming their N-band spectra through an NxN transformation matrix, which is derived by mapping the representative samples of their original spectra to the spectra of their target colors using least mean square method. On the other hand, the color of the tissue structure of interest is modified by modulating the transformed spectra with the sum of the pixel’s spectral residual-errors at specific bands weighted through an NxN weighting matrix; the spectral error is derived by taking the difference between the pixel’s original spectrum and its reconstructed spectrum using the first M dominant principal component vectors in principal component analysis. Promising results were obtained on the visualization of the collagen fiber and the non-collagen tissue structures, e.g., nuclei, cytoplasm and red blood cells (RBC), in a hematoxylin and eosin (H&E) stained image.http://dx.doi.org/10.3233/ACP-2012-0069 |
| spellingShingle | Pinky A. Bautista Yukako Yagi Multispectral Enhancement Method to Increase the Visual Differences of Tissue Structures in Stained Histopathology Images Analytical Cellular Pathology |
| title | Multispectral Enhancement Method to Increase the Visual Differences of Tissue Structures in Stained Histopathology Images |
| title_full | Multispectral Enhancement Method to Increase the Visual Differences of Tissue Structures in Stained Histopathology Images |
| title_fullStr | Multispectral Enhancement Method to Increase the Visual Differences of Tissue Structures in Stained Histopathology Images |
| title_full_unstemmed | Multispectral Enhancement Method to Increase the Visual Differences of Tissue Structures in Stained Histopathology Images |
| title_short | Multispectral Enhancement Method to Increase the Visual Differences of Tissue Structures in Stained Histopathology Images |
| title_sort | multispectral enhancement method to increase the visual differences of tissue structures in stained histopathology images |
| url | http://dx.doi.org/10.3233/ACP-2012-0069 |
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