Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol
In the changing global environmental scenarios, water scarcity and recurrent drought impose huge reductions to the peanut (Arachis hypogaea L.) crop yield. In plants, osmotic adjustments associated with efficient free radical scavenging ability during abiotic stress are important components of stres...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/125967 |
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| author | Tengale Dipak Bhauso Thankappan Radhakrishnan Abhay Kumar Gyan Prakash Mishra Jentilal Ramjibhai Dobaria Kirankumar Patel Manchikatla Venkat Rajam |
| author_facet | Tengale Dipak Bhauso Thankappan Radhakrishnan Abhay Kumar Gyan Prakash Mishra Jentilal Ramjibhai Dobaria Kirankumar Patel Manchikatla Venkat Rajam |
| author_sort | Tengale Dipak Bhauso |
| collection | DOAJ |
| description | In the changing global environmental scenarios, water scarcity and recurrent drought impose huge reductions to the peanut (Arachis hypogaea L.) crop yield. In plants, osmotic adjustments associated with efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms. Mannitol, a compatible solute, is known to scavenge hydroxyl radicals generated during various abiotic stresses, thereby conferring tolerance to water-deficit stress in many plant species. However, peanut plant is not known to synthesize mannitol. Therefore, bacterial mtlD gene coding for mannitol 1-phosphate dehydrogenase under the control of constitutive promoter CaMV35S was introduced and overexpressed in the peanut cv. GG 20 using Agrobacterium tumefaciens-mediated transformation. A total of eight independent transgenic events were confirmed at molecular level by PCR, Southern blotting, and RT-PCR. Transgenic lines had increased amount of mannitol and exhibited enhanced tolerance in response to water-deficit stress. Improved performance of the mtlD transgenics was indicated by excised-leaf water loss assay and relative water content under water-deficit stress. Better performance of transgenics was due to the ability of the plants to synthesize mannitol. However, regulation of mtlD gene expression in transgenic plants remains to be elucidated. |
| format | Article |
| id | doaj-art-04b1907fd4994c2f8292c917fd97833a |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-04b1907fd4994c2f8292c917fd97833a2025-02-03T01:26:15ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/125967125967Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of MannitolTengale Dipak Bhauso0Thankappan Radhakrishnan1Abhay Kumar2Gyan Prakash Mishra3Jentilal Ramjibhai Dobaria4Kirankumar Patel5Manchikatla Venkat Rajam6Directorate of Groundnut Research, Post Box No. 5, Junagadh, Gujarat 362001, IndiaDirectorate of Groundnut Research, Post Box No. 5, Junagadh, Gujarat 362001, IndiaDirectorate of Groundnut Research, Post Box No. 5, Junagadh, Gujarat 362001, IndiaDirectorate of Groundnut Research, Post Box No. 5, Junagadh, Gujarat 362001, IndiaDirectorate of Groundnut Research, Post Box No. 5, Junagadh, Gujarat 362001, IndiaDirectorate of Groundnut Research, Post Box No. 5, Junagadh, Gujarat 362001, IndiaUniversity of Delhi, Benito Juarez Road, New Delhi 110021, IndiaIn the changing global environmental scenarios, water scarcity and recurrent drought impose huge reductions to the peanut (Arachis hypogaea L.) crop yield. In plants, osmotic adjustments associated with efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms. Mannitol, a compatible solute, is known to scavenge hydroxyl radicals generated during various abiotic stresses, thereby conferring tolerance to water-deficit stress in many plant species. However, peanut plant is not known to synthesize mannitol. Therefore, bacterial mtlD gene coding for mannitol 1-phosphate dehydrogenase under the control of constitutive promoter CaMV35S was introduced and overexpressed in the peanut cv. GG 20 using Agrobacterium tumefaciens-mediated transformation. A total of eight independent transgenic events were confirmed at molecular level by PCR, Southern blotting, and RT-PCR. Transgenic lines had increased amount of mannitol and exhibited enhanced tolerance in response to water-deficit stress. Improved performance of the mtlD transgenics was indicated by excised-leaf water loss assay and relative water content under water-deficit stress. Better performance of transgenics was due to the ability of the plants to synthesize mannitol. However, regulation of mtlD gene expression in transgenic plants remains to be elucidated.http://dx.doi.org/10.1155/2014/125967 |
| spellingShingle | Tengale Dipak Bhauso Thankappan Radhakrishnan Abhay Kumar Gyan Prakash Mishra Jentilal Ramjibhai Dobaria Kirankumar Patel Manchikatla Venkat Rajam Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol The Scientific World Journal |
| title | Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol |
| title_full | Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol |
| title_fullStr | Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol |
| title_full_unstemmed | Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol |
| title_short | Overexpression of Bacterial mtlD Gene in Peanut Improves Drought Tolerance through Accumulation of Mannitol |
| title_sort | overexpression of bacterial mtld gene in peanut improves drought tolerance through accumulation of mannitol |
| url | http://dx.doi.org/10.1155/2014/125967 |
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