UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca
The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) o...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2022-03-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2022-0001 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832573944623792128 |
|---|---|
| author | Massadeh Rafeef K. El-Elimat Tamam Al-Gharaibeh Mohammad Tawaha Khaled Alali Feras Q. |
| author_facet | Massadeh Rafeef K. El-Elimat Tamam Al-Gharaibeh Mohammad Tawaha Khaled Alali Feras Q. |
| author_sort | Massadeh Rafeef K. |
| collection | DOAJ |
| description | The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g−1 dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of N. glauca. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (e.g., β-bisabolol) and carboxylic acids and esters (e.g., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected. |
| format | Article |
| id | doaj-art-049e03f3bc9f4463864fbe7d6d435e86 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-049e03f3bc9f4463864fbe7d6d435e862025-02-02T01:41:29ZengSciendoActa Pharmaceutica1846-95582022-03-017219710810.2478/acph-2022-0001UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glaucaMassadeh Rafeef K.0El-Elimat Tamam1Al-Gharaibeh Mohammad2Tawaha Khaled3Alali Feras Q.4Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid22110, JordanDepartment of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid22110, JordanDepartment of Plant Production, Faculty of Agriculture, Jordan University of Science and Technology, Irbid22110, JordanDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman11942, JordanCollege of Pharmacy, QU Health, Qatar University, Doha2713, QatarThe alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g−1 dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of N. glauca. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (e.g., β-bisabolol) and carboxylic acids and esters (e.g., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.https://doi.org/10.2478/acph-2022-0001nicotiana glaucatobacco treeuplc-hresimsgc-msanabasinenicotinevolatile oilmethanolic extract |
| spellingShingle | Massadeh Rafeef K. El-Elimat Tamam Al-Gharaibeh Mohammad Tawaha Khaled Alali Feras Q. UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca Acta Pharmaceutica nicotiana glauca tobacco tree uplc-hresims gc-ms anabasine nicotine volatile oil methanolic extract |
| title | UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca |
| title_full | UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca |
| title_fullStr | UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca |
| title_full_unstemmed | UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca |
| title_short | UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca |
| title_sort | uplc hresi ms and gc ms analysis of the leaves of nicotiana glauca |
| topic | nicotiana glauca tobacco tree uplc-hresims gc-ms anabasine nicotine volatile oil methanolic extract |
| url | https://doi.org/10.2478/acph-2022-0001 |
| work_keys_str_mv | AT massadehrafeefk uplchresimsandgcmsanalysisoftheleavesofnicotianaglauca AT elelimattamam uplchresimsandgcmsanalysisoftheleavesofnicotianaglauca AT algharaibehmohammad uplchresimsandgcmsanalysisoftheleavesofnicotianaglauca AT tawahakhaled uplchresimsandgcmsanalysisoftheleavesofnicotianaglauca AT alaliferasq uplchresimsandgcmsanalysisoftheleavesofnicotianaglauca |