The Response of Corneal Endothelial Cells to Shear Stress in an In Vitro Flow Model
Purpose. Corneal endothelial cells are usually exposed to shear stress caused by the aqueous humour, which is similar to the exposure of vascular endothelial cells to shear stress caused by blood flow. However, the effect of fluid shear stress on corneal endothelial cells is still poorly understood....
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2021/9217866 |
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| _version_ | 1832565624091443200 |
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| author | Sujuan Duan Yingjie Li Yanyan Zhang Xuan Zhu Yan Mei Dongmei Xu Guofu Huang |
| author_facet | Sujuan Duan Yingjie Li Yanyan Zhang Xuan Zhu Yan Mei Dongmei Xu Guofu Huang |
| author_sort | Sujuan Duan |
| collection | DOAJ |
| description | Purpose. Corneal endothelial cells are usually exposed to shear stress caused by the aqueous humour, which is similar to the exposure of vascular endothelial cells to shear stress caused by blood flow. However, the effect of fluid shear stress on corneal endothelial cells is still poorly understood. The purpose of this study was to explore whether the shear stress that results from the aqueous humour influences corneal endothelial cells. Methods. An in vitro model was established to generate fluid flow on cells, and the effect of fluid flow on corneal endothelial cells after exposure to two levels of shear stress for different durations was investigated. The mRNA and protein expression of corneal endothelium-related markers in rabbit corneal endothelial cells was evaluated by real-time PCR and western blotting. Results. The expression of the corneal endothelium-related markers ZO-1, N-cadherin, and Na+-K+-ATPase in rabbit corneal endothelial cells (RCECs) was upregulated at both the mRNA and protein levels after exposure to shear stress. Conclusion. This study demonstrates that RCECs respond favourably to fluid shear stress, which may contribute to the maintenance of corneal endothelial cell function. Furthermore, this study also provides a theoretical foundation for further investigating the response of human corneal endothelial cells to the shear stress caused by the aqueous humour. |
| format | Article |
| id | doaj-art-0467de71ce68479db7e2ecbe0d1c261e |
| institution | Kabale University |
| issn | 2090-0058 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-0467de71ce68479db7e2ecbe0d1c261e2025-02-03T01:07:06ZengWileyJournal of Ophthalmology2090-00582021-01-01202110.1155/2021/9217866The Response of Corneal Endothelial Cells to Shear Stress in an In Vitro Flow ModelSujuan Duan0Yingjie Li1Yanyan Zhang2Xuan Zhu3Yan Mei4Dongmei Xu5Guofu Huang6Department of OphthalmologyDepartment of OphthalmologyMedical Department of Graduate SchoolDepartment of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyDepartment of OphthalmologyPurpose. Corneal endothelial cells are usually exposed to shear stress caused by the aqueous humour, which is similar to the exposure of vascular endothelial cells to shear stress caused by blood flow. However, the effect of fluid shear stress on corneal endothelial cells is still poorly understood. The purpose of this study was to explore whether the shear stress that results from the aqueous humour influences corneal endothelial cells. Methods. An in vitro model was established to generate fluid flow on cells, and the effect of fluid flow on corneal endothelial cells after exposure to two levels of shear stress for different durations was investigated. The mRNA and protein expression of corneal endothelium-related markers in rabbit corneal endothelial cells was evaluated by real-time PCR and western blotting. Results. The expression of the corneal endothelium-related markers ZO-1, N-cadherin, and Na+-K+-ATPase in rabbit corneal endothelial cells (RCECs) was upregulated at both the mRNA and protein levels after exposure to shear stress. Conclusion. This study demonstrates that RCECs respond favourably to fluid shear stress, which may contribute to the maintenance of corneal endothelial cell function. Furthermore, this study also provides a theoretical foundation for further investigating the response of human corneal endothelial cells to the shear stress caused by the aqueous humour.http://dx.doi.org/10.1155/2021/9217866 |
| spellingShingle | Sujuan Duan Yingjie Li Yanyan Zhang Xuan Zhu Yan Mei Dongmei Xu Guofu Huang The Response of Corneal Endothelial Cells to Shear Stress in an In Vitro Flow Model Journal of Ophthalmology |
| title | The Response of Corneal Endothelial Cells to Shear Stress in an In Vitro Flow Model |
| title_full | The Response of Corneal Endothelial Cells to Shear Stress in an In Vitro Flow Model |
| title_fullStr | The Response of Corneal Endothelial Cells to Shear Stress in an In Vitro Flow Model |
| title_full_unstemmed | The Response of Corneal Endothelial Cells to Shear Stress in an In Vitro Flow Model |
| title_short | The Response of Corneal Endothelial Cells to Shear Stress in an In Vitro Flow Model |
| title_sort | response of corneal endothelial cells to shear stress in an in vitro flow model |
| url | http://dx.doi.org/10.1155/2021/9217866 |
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